Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13545 and PS417_13550 are separated by 25 nucleotides PS417_13550 and PS417_13555 are separated by 2 nucleotides PS417_13555 and PS417_13560 are separated by 7 nucleotides PS417_13560 and PS417_13565 overlap by 4 nucleotides
PS417_13545: PS417_13545 - arginine aminotransferase, at 2,984,297 to 2,986,291
_13545
PS417_13550: PS417_13550 - amino acid ABC transporter substrate-binding protein, at 2,986,317 to 2,987,171
_13550
PS417_13555: PS417_13555 - ABC transporter permease, at 2,987,174 to 2,987,878
_13555
PS417_13560: PS417_13560 - nickel permease, at 2,987,886 to 2,988,584
_13560
PS417_13565: PS417_13565 - permease, at 2,988,581 to 2,989,579
_13565
Position (kb)
2987
2988 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2986.647 kb on + strand, within PS417_13550 at 2986.648 kb on - strand, within PS417_13550 at 2986.648 kb on - strand at 2986.650 kb on + strand, within PS417_13550 at 2986.650 kb on + strand, within PS417_13550 at 2986.664 kb on - strand, within PS417_13550 at 2986.761 kb on + strand, within PS417_13550 at 2986.761 kb on + strand, within PS417_13550 at 2986.762 kb on - strand, within PS417_13550 at 2986.774 kb on - strand, within PS417_13550 at 2986.774 kb on - strand, within PS417_13550 at 2986.800 kb on + strand, within PS417_13550 at 2986.801 kb on - strand, within PS417_13550 at 2987.046 kb on + strand, within PS417_13550 at 2987.400 kb on - strand, within PS417_13555 at 2987.400 kb on - strand, within PS417_13555 at 2987.403 kb on - strand, within PS417_13555 at 2987.774 kb on + strand, within PS417_13555 at 2987.795 kb on + strand, within PS417_13555 at 2987.795 kb on + strand, within PS417_13555 at 2987.795 kb on + strand, within PS417_13555 at 2987.796 kb on - strand, within PS417_13555 at 2987.796 kb on - strand, within PS417_13555 at 2987.823 kb on - strand at 2987.823 kb on - strand at 2987.899 kb on + strand at 2987.904 kb on + strand at 2987.904 kb on + strand at 2987.905 kb on - strand at 2988.048 kb on + strand, within PS417_13560 at 2988.100 kb on - strand, within PS417_13560 at 2988.163 kb on - strand, within PS417_13560 at 2988.183 kb on + strand, within PS417_13560 at 2988.184 kb on - strand, within PS417_13560 at 2988.219 kb on + strand, within PS417_13560 at 2988.219 kb on + strand, within PS417_13560 at 2988.471 kb on + strand, within PS417_13560 at 2988.471 kb on + strand, within PS417_13560 at 2988.471 kb on + strand, within PS417_13560 at 2988.472 kb on - strand, within PS417_13560 at 2988.502 kb on - strand, within PS417_13560 at 2988.502 kb on - strand, within PS417_13560 at 2988.681 kb on - strand, within PS417_13565 at 2988.839 kb on + strand, within PS417_13565 at 2988.840 kb on - strand, within PS417_13565 at 2988.840 kb on - strand, within PS417_13565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,986,647 + PS417_13550 0.39 +0.2 2,986,648 - PS417_13550 0.39 -0.1 2,986,648 - -0.6 2,986,650 + PS417_13550 0.39 +1.3 2,986,650 + PS417_13550 0.39 -0.3 2,986,664 - PS417_13550 0.41 -0.1 2,986,761 + PS417_13550 0.52 +0.3 2,986,761 + PS417_13550 0.52 -0.5 2,986,762 - PS417_13550 0.52 -0.4 2,986,774 - PS417_13550 0.53 +0.6 2,986,774 - PS417_13550 0.53 -0.1 2,986,800 + PS417_13550 0.56 -1.2 2,986,801 - PS417_13550 0.57 -3.4 2,987,046 + PS417_13550 0.85 +1.7 2,987,400 - PS417_13555 0.32 -2.0 2,987,400 - PS417_13555 0.32 -0.4 2,987,403 - PS417_13555 0.32 -2.1 2,987,774 + PS417_13555 0.85 -1.6 2,987,795 + PS417_13555 0.88 -0.4 2,987,795 + PS417_13555 0.88 -1.1 2,987,795 + PS417_13555 0.88 +1.3 2,987,796 - PS417_13555 0.88 +0.7 2,987,796 - PS417_13555 0.88 -1.2 2,987,823 - +4.4 2,987,823 - +0.6 2,987,899 + -2.7 2,987,904 + +0.1 2,987,904 + +0.4 2,987,905 - +0.8 2,988,048 + PS417_13560 0.23 +0.3 2,988,100 - PS417_13560 0.31 +0.7 2,988,163 - PS417_13560 0.40 +0.5 2,988,183 + PS417_13560 0.42 +0.3 2,988,184 - PS417_13560 0.43 -0.1 2,988,219 + PS417_13560 0.48 -0.4 2,988,219 + PS417_13560 0.48 +1.3 2,988,471 + PS417_13560 0.84 -0.6 2,988,471 + PS417_13560 0.84 +1.3 2,988,471 + PS417_13560 0.84 -0.2 2,988,472 - PS417_13560 0.84 -0.3 2,988,502 - PS417_13560 0.88 -0.1 2,988,502 - PS417_13560 0.88 -1.9 2,988,681 - PS417_13565 0.10 +1.6 2,988,839 + PS417_13565 0.26 +0.8 2,988,840 - PS417_13565 0.26 +1.3 2,988,840 - PS417_13565 0.26 +1.1
Or see this region's nucleotide sequence