Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12465 and PS417_12470 are separated by 108 nucleotides PS417_12470 and PS417_12475 are separated by 50 nucleotides PS417_12475 and PS417_12480 are separated by 40 nucleotides
PS417_12465: PS417_12465 - Fis family transcriptional regulator, at 2,710,807 to 2,712,132
_12465
PS417_12470: PS417_12470 - hypothetical protein, at 2,712,241 to 2,713,542
_12470
PS417_12475: PS417_12475 - hypothetical protein, at 2,713,593 to 2,714,336
_12475
PS417_12480: PS417_12480 - peptidase C39, at 2,714,377 to 2,715,057
_12480
Position (kb)
2712
2713
2714 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2711.544 kb on - strand, within PS417_12465 at 2711.544 kb on - strand, within PS417_12465 at 2711.693 kb on - strand, within PS417_12465 at 2711.693 kb on - strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.753 kb on + strand, within PS417_12465 at 2711.786 kb on - strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.927 kb on + strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.928 kb on - strand, within PS417_12465 at 2711.983 kb on - strand, within PS417_12465 at 2712.249 kb on - strand at 2712.249 kb on - strand at 2712.365 kb on + strand at 2712.465 kb on + strand at 2712.493 kb on + strand, within PS417_12470 at 2712.591 kb on + strand, within PS417_12470 at 2712.599 kb on + strand, within PS417_12470 at 2712.704 kb on + strand, within PS417_12470 at 2712.704 kb on + strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.705 kb on - strand, within PS417_12470 at 2712.859 kb on - strand, within PS417_12470 at 2712.923 kb on + strand, within PS417_12470 at 2712.987 kb on - strand, within PS417_12470 at 2713.041 kb on + strand, within PS417_12470 at 2713.056 kb on + strand, within PS417_12470 at 2713.056 kb on + strand, within PS417_12470 at 2713.057 kb on - strand, within PS417_12470 at 2713.112 kb on + strand, within PS417_12470 at 2713.112 kb on + strand, within PS417_12470 at 2713.173 kb on - strand, within PS417_12470 at 2713.475 kb on + strand at 2713.475 kb on + strand at 2713.475 kb on + strand at 2713.500 kb on + strand at 2713.501 kb on - strand at 2713.501 kb on - strand at 2713.501 kb on - strand at 2713.523 kb on - strand at 2713.547 kb on + strand at 2713.589 kb on + strand at 2713.590 kb on - strand at 2713.743 kb on - strand, within PS417_12475 at 2713.778 kb on + strand, within PS417_12475 at 2713.938 kb on + strand, within PS417_12475 at 2713.938 kb on + strand, within PS417_12475 at 2713.938 kb on + strand, within PS417_12475 at 2713.939 kb on - strand, within PS417_12475 at 2713.939 kb on - strand at 2713.939 kb on - strand, within PS417_12475 at 2713.948 kb on + strand, within PS417_12475 at 2713.955 kb on + strand, within PS417_12475 at 2713.955 kb on + strand, within PS417_12475 at 2713.973 kb on + strand, within PS417_12475 at 2713.973 kb on + strand, within PS417_12475 at 2714.082 kb on + strand, within PS417_12475 at 2714.082 kb on + strand, within PS417_12475 at 2714.083 kb on - strand, within PS417_12475 at 2714.086 kb on + strand, within PS417_12475 at 2714.087 kb on - strand, within PS417_12475 at 2714.144 kb on + strand, within PS417_12475 at 2714.145 kb on - strand, within PS417_12475 at 2714.196 kb on - strand, within PS417_12475 at 2714.216 kb on - strand, within PS417_12475 at 2714.282 kb on - strand at 2714.282 kb on - strand at 2714.364 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,711,544 - PS417_12465 0.56 -0.4 2,711,544 - PS417_12465 0.56 +0.4 2,711,693 - PS417_12465 0.67 +0.7 2,711,693 - PS417_12465 0.67 -0.1 2,711,753 + PS417_12465 0.71 -0.3 2,711,753 + PS417_12465 0.71 -0.7 2,711,753 + PS417_12465 0.71 -1.7 2,711,786 - PS417_12465 0.74 +1.4 2,711,927 + PS417_12465 0.84 -2.6 2,711,927 + PS417_12465 0.84 -0.9 2,711,927 + PS417_12465 0.84 +0.3 2,711,927 + PS417_12465 0.84 +0.4 2,711,928 - PS417_12465 0.85 +0.0 2,711,928 - PS417_12465 0.85 +0.1 2,711,928 - PS417_12465 0.85 +1.6 2,711,983 - PS417_12465 0.89 -1.4 2,712,249 - +2.8 2,712,249 - +0.5 2,712,365 + +1.7 2,712,465 + +0.7 2,712,493 + PS417_12470 0.19 +0.4 2,712,591 + PS417_12470 0.27 -2.1 2,712,599 + PS417_12470 0.27 -0.8 2,712,704 + PS417_12470 0.36 +0.0 2,712,704 + PS417_12470 0.36 -1.3 2,712,705 - PS417_12470 0.36 +0.9 2,712,705 - PS417_12470 0.36 +0.9 2,712,705 - PS417_12470 0.36 -0.0 2,712,859 - PS417_12470 0.47 +0.8 2,712,923 + PS417_12470 0.52 -0.5 2,712,987 - PS417_12470 0.57 +0.4 2,713,041 + PS417_12470 0.61 -0.2 2,713,056 + PS417_12470 0.63 +1.2 2,713,056 + PS417_12470 0.63 +0.1 2,713,057 - PS417_12470 0.63 -1.1 2,713,112 + PS417_12470 0.67 +0.1 2,713,112 + PS417_12470 0.67 +0.7 2,713,173 - PS417_12470 0.72 +1.4 2,713,475 + +1.3 2,713,475 + -3.0 2,713,475 + -0.6 2,713,500 + -1.0 2,713,501 - +0.1 2,713,501 - -0.3 2,713,501 - +0.4 2,713,523 - +2.1 2,713,547 + -0.9 2,713,589 + -0.2 2,713,590 - -3.2 2,713,743 - PS417_12475 0.20 -0.2 2,713,778 + PS417_12475 0.25 -1.8 2,713,938 + PS417_12475 0.46 -0.3 2,713,938 + PS417_12475 0.46 -2.7 2,713,938 + PS417_12475 0.46 +1.9 2,713,939 - PS417_12475 0.47 +0.2 2,713,939 - -0.2 2,713,939 - PS417_12475 0.47 -1.8 2,713,948 + PS417_12475 0.48 +1.0 2,713,955 + PS417_12475 0.49 -0.5 2,713,955 + PS417_12475 0.49 -1.2 2,713,973 + PS417_12475 0.51 -1.4 2,713,973 + PS417_12475 0.51 -0.1 2,714,082 + PS417_12475 0.66 -1.0 2,714,082 + PS417_12475 0.66 +0.7 2,714,083 - PS417_12475 0.66 +0.1 2,714,086 + PS417_12475 0.66 +0.3 2,714,087 - PS417_12475 0.66 +1.7 2,714,144 + PS417_12475 0.74 -0.7 2,714,145 - PS417_12475 0.74 +0.1 2,714,196 - PS417_12475 0.81 +0.2 2,714,216 - PS417_12475 0.84 -3.2 2,714,282 - -0.3 2,714,282 - +0.9 2,714,364 + +0.3
Or see this region's nucleotide sequence