Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11980 and PS417_11990 are separated by 350 nucleotides PS417_11990 and PS417_11995 are separated by 15 nucleotides PS417_11995 and PS417_12000 overlap by 4 nucleotides PS417_12000 and PS417_12005 overlap by 4 nucleotides PS417_12005 and PS417_12010 are separated by 56 nucleotides
PS417_11980: PS417_11980 - hypothetical protein, at 2,607,077 to 2,607,325
_11980
PS417_11990: PS417_11990 - hypothetical protein, at 2,607,676 to 2,607,987
_11990
PS417_11995: PS417_11995 - RNA polymerase sigma factor, at 2,608,003 to 2,608,581
_11995
PS417_12000: PS417_12000 - hypothetical protein, at 2,608,578 to 2,609,009
_12000
PS417_12005: PS417_12005 - hypothetical protein, at 2,609,006 to 2,609,494
_12005
PS417_12010: PS417_12010 - SCP-2 family sterol carrier protein, at 2,609,551 to 2,609,865
_12010
Position (kb)
2608
2609 Strain fitness (log2 ratio)
-1
0
1 at 2607.024 kb on + strand at 2607.024 kb on + strand at 2607.025 kb on - strand at 2607.025 kb on - strand at 2607.025 kb on - strand at 2607.025 kb on - strand at 2607.025 kb on - strand at 2607.025 kb on - strand at 2607.365 kb on + strand at 2607.365 kb on + strand at 2607.446 kb on + strand at 2607.587 kb on - strand at 2608.037 kb on + strand at 2608.038 kb on - strand at 2608.038 kb on - strand at 2608.429 kb on + strand, within PS417_11995 at 2609.167 kb on - strand, within PS417_12005 at 2609.210 kb on - strand, within PS417_12005 at 2609.210 kb on - strand, within PS417_12005 at 2609.432 kb on + strand, within PS417_12005 at 2609.493 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,607,024 + -0.8 2,607,024 + -0.4 2,607,025 - -0.8 2,607,025 - -0.6 2,607,025 - +0.5 2,607,025 - -0.6 2,607,025 - -0.5 2,607,025 - -0.4 2,607,365 + -1.4 2,607,365 + +1.0 2,607,446 + -0.3 2,607,587 - +0.1 2,608,037 + -0.4 2,608,038 - -1.3 2,608,038 - -0.1 2,608,429 + PS417_11995 0.74 +0.5 2,609,167 - PS417_12005 0.33 +0.5 2,609,210 - PS417_12005 0.42 +1.1 2,609,210 - PS417_12005 0.42 +0.5 2,609,432 + PS417_12005 0.87 +0.1 2,609,493 - -0.6
Or see this region's nucleotide sequence