Strain Fitness in Pseudomonas simiae WCS417 around PS417_01040

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_01035 and PS417_01040 are separated by 121 nucleotidesPS417_01040 and PS417_01045 overlap by 4 nucleotides PS417_01035: PS417_01035 - ATP-binding protein, at 224,898 to 226,058 _01035 PS417_01040: PS417_01040 - RND transporter, at 226,180 to 227,262 _01040 PS417_01045: PS417_01045 - RND transporter, at 227,259 to 228,329 _01045 Position (kb) 226 227 228Strain fitness (log2 ratio) -2 -1 0 1 2 3at 225.184 kb on + strand, within PS417_01035at 225.553 kb on + strand, within PS417_01035at 225.553 kb on + strand, within PS417_01035at 225.554 kb on - strand, within PS417_01035at 225.554 kb on - strand, within PS417_01035at 226.110 kb on + strandat 226.125 kb on + strandat 226.126 kb on - strandat 226.339 kb on - strand, within PS417_01040at 226.542 kb on - strand, within PS417_01040at 226.570 kb on + strand, within PS417_01040at 226.570 kb on + strand, within PS417_01040at 226.570 kb on + strand, within PS417_01040at 226.571 kb on - strand, within PS417_01040at 226.746 kb on - strand, within PS417_01040at 226.811 kb on - strand, within PS417_01040at 226.811 kb on - strand, within PS417_01040at 226.811 kb on - strand, within PS417_01040at 226.929 kb on + strand, within PS417_01040at 227.583 kb on + strand, within PS417_01045at 227.583 kb on + strand, within PS417_01045at 227.583 kb on + strand, within PS417_01045at 227.584 kb on - strand, within PS417_01045at 227.584 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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225,184 + PS417_01035 0.25 +0.7
225,553 + PS417_01035 0.56 +1.3
225,553 + PS417_01035 0.56 +1.6
225,554 - PS417_01035 0.57 -0.2
225,554 - PS417_01035 0.57 -0.6
226,110 + -2.6
226,125 + +0.6
226,126 - +0.6
226,339 - PS417_01040 0.15 -0.8
226,542 - PS417_01040 0.33 +0.7
226,570 + PS417_01040 0.36 -0.9
226,570 + PS417_01040 0.36 -1.5
226,570 + PS417_01040 0.36 -0.0
226,571 - PS417_01040 0.36 -0.0
226,746 - PS417_01040 0.52 -1.0
226,811 - PS417_01040 0.58 -2.0
226,811 - PS417_01040 0.58 -0.3
226,811 - PS417_01040 0.58 -0.3
226,929 + PS417_01040 0.69 -0.9
227,583 + PS417_01045 0.30 -0.3
227,583 + PS417_01045 0.30 -2.1
227,583 + PS417_01045 0.30 +0.0
227,584 - PS417_01045 0.30 +3.6
227,584 - +0.6

Or see this region's nucleotide sequence