Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09325 and PS417_09330 are separated by 105 nucleotides PS417_09330 and PS417_09335 are separated by 144 nucleotides PS417_09335 and PS417_09340 are separated by 30 nucleotides
PS417_09325: PS417_09325 - peptidase S41, at 2,022,777 to 2,024,891
_09325
PS417_09330: PS417_09330 - NAD(P)H-quinone oxidoreductase, at 2,024,997 to 2,025,959
_09330
PS417_09335: PS417_09335 - HAD family hydrolase, at 2,026,104 to 2,026,757
_09335
PS417_09340: PS417_09340 - spermidine/putrescine ABC transporter ATP-binding protein, at 2,026,788 to 2,027,777
_09340
Position (kb)
2024
2025
2026 Strain fitness (log2 ratio)
-2
-1
0
1 at 2024.157 kb on - strand, within PS417_09325 at 2024.170 kb on + strand, within PS417_09325 at 2024.171 kb on - strand, within PS417_09325 at 2024.516 kb on - strand, within PS417_09325 at 2024.522 kb on + strand, within PS417_09325 at 2024.891 kb on + strand at 2024.891 kb on + strand at 2024.891 kb on + strand at 2024.892 kb on - strand at 2024.894 kb on + strand at 2024.894 kb on + strand at 2024.958 kb on - strand at 2025.053 kb on + strand at 2025.351 kb on + strand, within PS417_09330 at 2025.624 kb on + strand, within PS417_09330 at 2025.624 kb on + strand, within PS417_09330 at 2025.742 kb on + strand, within PS417_09330 at 2025.742 kb on + strand, within PS417_09330 at 2026.012 kb on + strand at 2026.334 kb on - strand, within PS417_09335 at 2026.794 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,024,157 - PS417_09325 0.65 +1.2 2,024,170 + PS417_09325 0.66 +0.4 2,024,171 - PS417_09325 0.66 -0.8 2,024,516 - PS417_09325 0.82 +0.1 2,024,522 + PS417_09325 0.83 +0.1 2,024,891 + -1.4 2,024,891 + -0.4 2,024,891 + +0.1 2,024,892 - -0.9 2,024,894 + +0.1 2,024,894 + -0.1 2,024,958 - +0.0 2,025,053 + +1.7 2,025,351 + PS417_09330 0.37 +1.2 2,025,624 + PS417_09330 0.65 -2.4 2,025,624 + PS417_09330 0.65 -1.5 2,025,742 + PS417_09330 0.77 -0.5 2,025,742 + PS417_09330 0.77 -2.1 2,026,012 + -1.4 2,026,334 - PS417_09335 0.35 -0.4 2,026,794 + +0.4
Or see this region's nucleotide sequence