Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08615 and PS417_08620 are separated by 91 nucleotides PS417_08620 and PS417_08625 overlap by 4 nucleotides PS417_08625 and PS417_08630 are separated by 2 nucleotides PS417_08630 and PS417_08635 are separated by 342 nucleotides
PS417_08615: PS417_08615 - diguanylate phosphodiesterase, at 1,882,975 to 1,884,138
_08615
PS417_08620: PS417_08620 - hypothetical protein, at 1,884,230 to 1,884,571
_08620
PS417_08625: PS417_08625 - membrane protein, at 1,884,568 to 1,884,951
_08625
PS417_08630: PS417_08630 - membrane protein, at 1,884,954 to 1,885,268
_08630
PS417_08635: PS417_08635 - ammonium transporter, at 1,885,611 to 1,886,819
_08635
Position (kb)
1884
1885 Strain fitness (log2 ratio)
-2
-1
0
1 at 1884.149 kb on + strand at 1884.149 kb on + strand at 1884.150 kb on - strand at 1884.421 kb on + strand, within PS417_08620 at 1884.686 kb on + strand, within PS417_08625 at 1884.687 kb on - strand, within PS417_08625 at 1885.272 kb on + strand at 1885.273 kb on - strand at 1885.432 kb on + strand at 1885.436 kb on + strand at 1885.514 kb on + strand at 1885.667 kb on + strand at 1885.787 kb on + strand, within PS417_08635 at 1885.788 kb on - strand, within PS417_08635 at 1885.793 kb on + strand, within PS417_08635 at 1885.793 kb on + strand, within PS417_08635 at 1885.793 kb on + strand, within PS417_08635 at 1885.803 kb on + strand, within PS417_08635 at 1885.804 kb on - strand, within PS417_08635 at 1885.813 kb on + strand, within PS417_08635 at 1885.813 kb on + strand, within PS417_08635 at 1885.831 kb on - strand, within PS417_08635
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,884,149 + +0.0 1,884,149 + +0.1 1,884,150 - -0.3 1,884,421 + PS417_08620 0.56 -1.2 1,884,686 + PS417_08625 0.31 +1.4 1,884,687 - PS417_08625 0.31 +0.0 1,885,272 + -0.1 1,885,273 - +1.0 1,885,432 + -0.6 1,885,436 + +0.6 1,885,514 + +0.2 1,885,667 + -0.3 1,885,787 + PS417_08635 0.15 +1.3 1,885,788 - PS417_08635 0.15 -2.3 1,885,793 + PS417_08635 0.15 +0.3 1,885,793 + PS417_08635 0.15 +0.7 1,885,793 + PS417_08635 0.15 +0.2 1,885,803 + PS417_08635 0.16 -1.5 1,885,804 - PS417_08635 0.16 +0.1 1,885,813 + PS417_08635 0.17 +1.1 1,885,813 + PS417_08635 0.17 -0.2 1,885,831 - PS417_08635 0.18 -1.8
Or see this region's nucleotide sequence