Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06695 and PS417_06700 are separated by 227 nucleotides PS417_06700 and PS417_06705 overlap by 1 nucleotides PS417_06705 and PS417_06710 overlap by 1 nucleotides
PS417_06695: PS417_06695 - 4-hydroxybenzoate transporter, at 1,482,981 to 1,484,321
_06695
PS417_06700: PS417_06700 - 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6) (from data) , at 1,484,549 to 1,485,406
_06700
PS417_06705: PS417_06705 - 3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6) (from data) , at 1,485,406 to 1,486,185
_06705
PS417_06710: PS417_06710 - beta-ketoadipyl CoA thiolase, at 1,486,185 to 1,487,387
_06710
Position (kb)
1484
1485
1486 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1483.755 kb on + strand, within PS417_06695 at 1483.789 kb on - strand, within PS417_06695 at 1483.812 kb on + strand, within PS417_06695 at 1484.035 kb on + strand, within PS417_06695 at 1484.036 kb on - strand, within PS417_06695 at 1484.378 kb on - strand at 1484.378 kb on - strand at 1484.698 kb on + strand, within PS417_06700 at 1484.880 kb on - strand, within PS417_06700 at 1485.255 kb on - strand, within PS417_06700 at 1485.255 kb on - strand, within PS417_06700 at 1485.278 kb on + strand at 1485.339 kb on - strand at 1485.404 kb on + strand at 1485.405 kb on - strand at 1485.405 kb on - strand at 1485.412 kb on + strand at 1485.765 kb on + strand, within PS417_06705 at 1485.770 kb on + strand, within PS417_06705 at 1485.770 kb on + strand, within PS417_06705 at 1485.771 kb on - strand, within PS417_06705 at 1485.771 kb on - strand, within PS417_06705 at 1486.196 kb on - strand at 1486.196 kb on - strand at 1486.200 kb on - strand at 1486.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,483,755 + PS417_06695 0.58 +1.0 1,483,789 - PS417_06695 0.60 -1.6 1,483,812 + PS417_06695 0.62 -0.9 1,484,035 + PS417_06695 0.79 -0.4 1,484,036 - PS417_06695 0.79 +0.7 1,484,378 - +0.2 1,484,378 - +0.1 1,484,698 + PS417_06700 0.17 -0.8 1,484,880 - PS417_06700 0.39 +0.6 1,485,255 - PS417_06700 0.82 -1.8 1,485,255 - PS417_06700 0.82 -1.1 1,485,278 + +0.0 1,485,339 - +2.9 1,485,404 + -2.1 1,485,405 - -1.3 1,485,405 - -0.6 1,485,412 + -0.5 1,485,765 + PS417_06705 0.46 -1.0 1,485,770 + PS417_06705 0.47 -0.8 1,485,770 + PS417_06705 0.47 +0.3 1,485,771 - PS417_06705 0.47 -0.4 1,485,771 - PS417_06705 0.47 +1.2 1,486,196 - -0.4 1,486,196 - -0.1 1,486,200 - -0.6 1,486,200 - -2.5
Or see this region's nucleotide sequence