Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06515 and PS417_06525 are separated by 80 nucleotides PS417_06525 and PS417_06530 are separated by 143 nucleotides
PS417_06515: PS417_06515 - beta-agarase, at 1,443,538 to 1,445,835
_06515
PS417_06525: PS417_06525 - beta-lactamase, at 1,445,916 to 1,447,061
_06525
PS417_06530: PS417_06530 - membrane protein, at 1,447,205 to 1,448,347
_06530
Position (kb)
1445
1446
1447
1448 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1445.056 kb on + strand, within PS417_06515 at 1445.057 kb on - strand, within PS417_06515 at 1445.185 kb on + strand, within PS417_06515 at 1445.212 kb on + strand, within PS417_06515 at 1445.264 kb on + strand, within PS417_06515 at 1445.265 kb on - strand, within PS417_06515 at 1445.268 kb on - strand, within PS417_06515 at 1445.321 kb on - strand, within PS417_06515 at 1445.343 kb on + strand, within PS417_06515 at 1445.343 kb on + strand, within PS417_06515 at 1445.344 kb on - strand, within PS417_06515 at 1445.344 kb on - strand, within PS417_06515 at 1445.347 kb on + strand, within PS417_06515 at 1445.347 kb on + strand, within PS417_06515 at 1445.347 kb on + strand, within PS417_06515 at 1445.348 kb on - strand, within PS417_06515 at 1445.348 kb on - strand, within PS417_06515 at 1445.348 kb on - strand at 1445.348 kb on - strand, within PS417_06515 at 1445.348 kb on - strand, within PS417_06515 at 1445.935 kb on - strand at 1445.957 kb on - strand at 1446.198 kb on + strand, within PS417_06525 at 1446.199 kb on - strand, within PS417_06525 at 1446.199 kb on - strand, within PS417_06525 at 1446.536 kb on + strand, within PS417_06525 at 1446.536 kb on + strand, within PS417_06525 at 1446.536 kb on + strand, within PS417_06525 at 1446.537 kb on - strand, within PS417_06525 at 1446.537 kb on - strand, within PS417_06525 at 1446.537 kb on - strand, within PS417_06525 at 1446.539 kb on - strand, within PS417_06525 at 1446.584 kb on - strand, within PS417_06525 at 1446.593 kb on - strand, within PS417_06525 at 1446.593 kb on - strand, within PS417_06525 at 1446.663 kb on - strand, within PS417_06525 at 1446.665 kb on + strand, within PS417_06525 at 1446.666 kb on - strand, within PS417_06525 at 1446.738 kb on + strand, within PS417_06525 at 1446.738 kb on + strand, within PS417_06525 at 1446.758 kb on + strand at 1446.758 kb on + strand, within PS417_06525 at 1446.758 kb on + strand, within PS417_06525 at 1446.968 kb on - strand at 1446.968 kb on - strand at 1446.990 kb on - strand at 1447.002 kb on + strand at 1447.002 kb on + strand at 1447.006 kb on + strand at 1447.006 kb on + strand at 1447.006 kb on + strand at 1447.007 kb on - strand at 1447.011 kb on - strand at 1447.011 kb on - strand at 1447.102 kb on - strand at 1447.103 kb on + strand at 1447.103 kb on + strand at 1447.199 kb on + strand at 1447.236 kb on + strand at 1447.246 kb on + strand at 1447.392 kb on + strand, within PS417_06530 at 1447.685 kb on + strand, within PS417_06530 at 1447.781 kb on + strand, within PS417_06530 at 1447.781 kb on + strand, within PS417_06530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,445,056 + PS417_06515 0.66 -0.7 1,445,057 - PS417_06515 0.66 +2.2 1,445,185 + PS417_06515 0.72 +2.0 1,445,212 + PS417_06515 0.73 -0.3 1,445,264 + PS417_06515 0.75 -0.7 1,445,265 - PS417_06515 0.75 +0.9 1,445,268 - PS417_06515 0.75 +1.4 1,445,321 - PS417_06515 0.78 +2.1 1,445,343 + PS417_06515 0.79 -1.1 1,445,343 + PS417_06515 0.79 +0.1 1,445,344 - PS417_06515 0.79 +0.9 1,445,344 - PS417_06515 0.79 +1.1 1,445,347 + PS417_06515 0.79 +0.5 1,445,347 + PS417_06515 0.79 -0.0 1,445,347 + PS417_06515 0.79 -1.7 1,445,348 - PS417_06515 0.79 +1.9 1,445,348 - PS417_06515 0.79 +0.9 1,445,348 - +2.0 1,445,348 - PS417_06515 0.79 +0.4 1,445,348 - PS417_06515 0.79 +0.8 1,445,935 - +1.1 1,445,957 - +0.2 1,446,198 + PS417_06525 0.25 -0.0 1,446,199 - PS417_06525 0.25 -1.0 1,446,199 - PS417_06525 0.25 +1.5 1,446,536 + PS417_06525 0.54 -0.4 1,446,536 + PS417_06525 0.54 -1.6 1,446,536 + PS417_06525 0.54 -1.2 1,446,537 - PS417_06525 0.54 +1.2 1,446,537 - PS417_06525 0.54 +1.5 1,446,537 - PS417_06525 0.54 +1.1 1,446,539 - PS417_06525 0.54 +0.4 1,446,584 - PS417_06525 0.58 +1.4 1,446,593 - PS417_06525 0.59 +1.2 1,446,593 - PS417_06525 0.59 +1.1 1,446,663 - PS417_06525 0.65 +0.7 1,446,665 + PS417_06525 0.65 -0.9 1,446,666 - PS417_06525 0.65 +1.0 1,446,738 + PS417_06525 0.72 +0.8 1,446,738 + PS417_06525 0.72 -1.0 1,446,758 + -1.0 1,446,758 + PS417_06525 0.73 -1.0 1,446,758 + PS417_06525 0.73 +0.6 1,446,968 - -0.1 1,446,968 - -0.1 1,446,990 - -0.8 1,447,002 + -1.5 1,447,002 + -1.4 1,447,006 + +2.2 1,447,006 + -0.1 1,447,006 + -3.4 1,447,007 - -0.2 1,447,011 - +1.5 1,447,011 - +1.0 1,447,102 - -1.1 1,447,103 + -0.4 1,447,103 + +0.3 1,447,199 + +0.2 1,447,236 + +0.4 1,447,246 + -0.5 1,447,392 + PS417_06530 0.16 +1.8 1,447,685 + PS417_06530 0.42 +0.4 1,447,781 + PS417_06530 0.50 +2.0 1,447,781 + PS417_06530 0.50 +0.6
Or see this region's nucleotide sequence