Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00580 and PS417_00585 are separated by 12 nucleotides PS417_00585 and PS417_00590 are separated by 412 nucleotides PS417_00590 and PS417_00595 are separated by 284 nucleotides
PS417_00580: PS417_00580 - acyl-CoA dehydrogenase, at 116,133 to 117,314
_00580
PS417_00585: PS417_00585 - CoA-transferase, at 117,327 to 118,547
_00585
PS417_00590: PS417_00590 - 5S ribosomal RNA, at 118,960 to 119,075
_00590
PS417_00595: PS417_00595 - chemotaxis protein, at 119,360 to 121,240
_00595
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 116.370 kb on + strand, within PS417_00580 at 116.503 kb on - strand, within PS417_00580 at 116.616 kb on + strand, within PS417_00580 at 116.950 kb on - strand, within PS417_00580 at 116.950 kb on - strand, within PS417_00580 at 116.950 kb on - strand, within PS417_00580 at 117.309 kb on + strand at 117.309 kb on + strand at 117.309 kb on + strand at 117.315 kb on + strand at 117.316 kb on - strand at 117.520 kb on - strand, within PS417_00585 at 117.522 kb on + strand, within PS417_00585 at 117.522 kb on + strand at 117.523 kb on - strand, within PS417_00585 at 117.705 kb on + strand, within PS417_00585 at 117.759 kb on + strand, within PS417_00585 at 118.000 kb on - strand, within PS417_00585 at 118.000 kb on - strand, within PS417_00585 at 118.125 kb on - strand, within PS417_00585 at 118.182 kb on - strand, within PS417_00585 at 118.469 kb on + strand at 118.470 kb on - strand at 118.492 kb on + strand at 118.493 kb on - strand at 118.493 kb on - strand at 118.541 kb on + strand at 118.559 kb on + strand at 118.559 kb on + strand at 118.560 kb on - strand at 118.560 kb on - strand at 118.560 kb on - strand at 118.560 kb on - strand at 118.561 kb on + strand at 118.561 kb on + strand at 118.563 kb on + strand at 118.563 kb on + strand at 118.563 kb on + strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.613 kb on - strand at 118.662 kb on - strand at 118.662 kb on - strand at 118.972 kb on - strand, within PS417_00590 at 118.972 kb on - strand, within PS417_00590 at 119.126 kb on - strand at 119.133 kb on + strand at 119.134 kb on - strand at 119.193 kb on - strand at 119.193 kb on - strand at 119.205 kb on + strand at 119.206 kb on - strand at 119.206 kb on - strand at 119.295 kb on + strand at 119.295 kb on + strand at 119.296 kb on - strand at 119.296 kb on - strand at 119.298 kb on - strand at 119.303 kb on + strand at 119.303 kb on + strand at 119.303 kb on + strand at 119.304 kb on - strand at 119.304 kb on - strand at 119.368 kb on + strand at 119.439 kb on - strand at 119.439 kb on - strand at 119.439 kb on - strand at 119.439 kb on - strand at 119.447 kb on + strand at 119.448 kb on - strand at 119.547 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 116,370 + PS417_00580 0.20 +0.8 116,503 - PS417_00580 0.31 -0.9 116,616 + PS417_00580 0.41 -0.8 116,950 - PS417_00580 0.69 +0.1 116,950 - PS417_00580 0.69 -0.1 116,950 - PS417_00580 0.69 -0.1 117,309 + +0.5 117,309 + +0.4 117,309 + +0.8 117,315 + -2.7 117,316 - +0.0 117,520 - PS417_00585 0.16 -0.3 117,522 + PS417_00585 0.16 +1.0 117,522 + +0.0 117,523 - PS417_00585 0.16 -1.2 117,705 + PS417_00585 0.31 +1.7 117,759 + PS417_00585 0.35 -0.3 118,000 - PS417_00585 0.55 +0.3 118,000 - PS417_00585 0.55 +0.4 118,125 - PS417_00585 0.65 +2.5 118,182 - PS417_00585 0.70 +0.7 118,469 + +0.8 118,470 - -0.7 118,492 + -1.2 118,493 - +1.2 118,493 - +0.1 118,541 + -2.0 118,559 + -0.9 118,559 + -0.0 118,560 - +1.1 118,560 - +0.3 118,560 - +0.1 118,560 - -0.7 118,561 + -0.1 118,561 + -1.2 118,563 + +1.4 118,563 + -0.9 118,563 + -0.5 118,564 - +0.5 118,564 - +0.5 118,564 - -0.3 118,564 - +0.4 118,564 - -2.6 118,564 - -1.8 118,564 - -0.6 118,613 - +0.7 118,662 - +0.2 118,662 - -0.3 118,972 - PS417_00590 0.10 +0.1 118,972 - PS417_00590 0.10 -0.3 119,126 - -1.9 119,133 + +0.6 119,134 - +2.2 119,193 - -0.4 119,193 - -1.2 119,205 + +0.0 119,206 - -3.2 119,206 - +1.2 119,295 + +1.9 119,295 + -1.2 119,296 - +0.7 119,296 - -0.5 119,298 - -0.3 119,303 + -0.3 119,303 + -1.2 119,303 + +0.0 119,304 - -1.4 119,304 - -0.7 119,368 + -0.9 119,439 - -0.1 119,439 - +0.0 119,439 - +0.6 119,439 - -1.1 119,447 + -1.7 119,448 - -0.4 119,547 + -1.2
Or see this region's nucleotide sequence