Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05475 and PS417_05480 overlap by 4 nucleotides PS417_05480 and PS417_05485 overlap by 4 nucleotides PS417_05485 and PS417_05490 are separated by 80 nucleotides
PS417_05475: PS417_05475 - arginase, at 1,233,548 to 1,234,468
_05475
PS417_05480: PS417_05480 - transporter, at 1,234,465 to 1,234,845
_05480
PS417_05485: PS417_05485 - transporter, at 1,234,842 to 1,235,246
_05485
PS417_05490: PS417_05490 - fatty acid desaturase, at 1,235,327 to 1,236,199
_05490
Position (kb)
1234
1235
1236 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1234.195 kb on - strand, within PS417_05475 at 1234.308 kb on + strand, within PS417_05475 at 1234.308 kb on + strand, within PS417_05475 at 1234.309 kb on - strand, within PS417_05475 at 1234.309 kb on - strand, within PS417_05475 at 1235.285 kb on + strand at 1235.285 kb on + strand at 1235.285 kb on + strand at 1235.511 kb on + strand, within PS417_05490 at 1235.512 kb on - strand, within PS417_05490 at 1235.512 kb on - strand, within PS417_05490 at 1235.524 kb on - strand, within PS417_05490 at 1235.577 kb on + strand, within PS417_05490 at 1235.577 kb on + strand at 1235.578 kb on - strand, within PS417_05490 at 1235.578 kb on - strand, within PS417_05490 at 1235.580 kb on + strand, within PS417_05490 at 1235.653 kb on - strand, within PS417_05490 at 1235.670 kb on + strand, within PS417_05490 at 1235.670 kb on + strand, within PS417_05490 at 1235.671 kb on - strand, within PS417_05490 at 1235.671 kb on - strand, within PS417_05490 at 1235.671 kb on - strand, within PS417_05490 at 1235.745 kb on + strand, within PS417_05490 at 1235.787 kb on + strand, within PS417_05490 at 1235.788 kb on - strand, within PS417_05490 at 1235.788 kb on - strand, within PS417_05490 at 1235.988 kb on + strand, within PS417_05490
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,234,195 - PS417_05475 0.70 +2.1 1,234,308 + PS417_05475 0.83 +0.8 1,234,308 + PS417_05475 0.83 +0.6 1,234,309 - PS417_05475 0.83 +1.4 1,234,309 - PS417_05475 0.83 +1.2 1,235,285 + -3.8 1,235,285 + +0.6 1,235,285 + -0.4 1,235,511 + PS417_05490 0.21 -2.0 1,235,512 - PS417_05490 0.21 +0.4 1,235,512 - PS417_05490 0.21 +0.2 1,235,524 - PS417_05490 0.23 -0.2 1,235,577 + PS417_05490 0.29 -0.0 1,235,577 + +0.6 1,235,578 - PS417_05490 0.29 +0.2 1,235,578 - PS417_05490 0.29 -0.2 1,235,580 + PS417_05490 0.29 +0.4 1,235,653 - PS417_05490 0.37 -0.2 1,235,670 + PS417_05490 0.39 -0.6 1,235,670 + PS417_05490 0.39 +0.1 1,235,671 - PS417_05490 0.39 -0.4 1,235,671 - PS417_05490 0.39 -0.4 1,235,671 - PS417_05490 0.39 -0.8 1,235,745 + PS417_05490 0.48 -0.3 1,235,787 + PS417_05490 0.53 +1.0 1,235,788 - PS417_05490 0.53 +1.6 1,235,788 - PS417_05490 0.53 -1.4 1,235,988 + PS417_05490 0.76 +0.3
Or see this region's nucleotide sequence