Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02050 and PS417_02055 overlap by 4 nucleotides PS417_02055 and PS417_02060 overlap by 4 nucleotides PS417_02060 and PS417_02065 are separated by 33 nucleotides
PS417_02050: PS417_02050 - hypothetical protein, at 459,972 to 460,454
_02050
PS417_02055: PS417_02055 - methyltransferase, at 460,451 to 461,185
_02055
PS417_02060: PS417_02060 - FAD-dependent oxidoreductase, at 461,182 to 462,429
_02060
PS417_02065: PS417_02065 - membrane protein, at 462,463 to 464,775
_02065
Position (kb) 461
462
463 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 460.716 kb on + strand, within PS417_02055 at 460.716 kb on + strand, within PS417_02055 at 460.717 kb on - strand, within PS417_02055 at 460.717 kb on - strand, within PS417_02055 at 460.746 kb on + strand, within PS417_02055 at 460.747 kb on - strand, within PS417_02055 at 461.059 kb on + strand, within PS417_02055 at 461.068 kb on + strand, within PS417_02055 at 461.459 kb on - strand, within PS417_02060 at 461.814 kb on + strand, within PS417_02060 at 461.815 kb on - strand, within PS417_02060 at 461.992 kb on + strand, within PS417_02060 at 462.226 kb on + strand, within PS417_02060 at 462.328 kb on + strand at 462.328 kb on + strand at 462.328 kb on + strand at 462.328 kb on + strand at 462.329 kb on - strand at 462.329 kb on - strand at 462.329 kb on - strand at 462.421 kb on + strand at 462.421 kb on + strand at 462.466 kb on + strand at 462.467 kb on - strand at 462.467 kb on - strand at 462.504 kb on + strand at 462.562 kb on - strand at 462.625 kb on + strand at 463.063 kb on + strand, within PS417_02065 at 463.063 kb on + strand, within PS417_02065 at 463.090 kb on - strand, within PS417_02065
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 460,716 + PS417_02055 0.36 +1.1 460,716 + PS417_02055 0.36 -0.2 460,717 - PS417_02055 0.36 +0.5 460,717 - PS417_02055 0.36 -1.9 460,746 + PS417_02055 0.40 +0.2 460,747 - PS417_02055 0.40 -0.5 461,059 + PS417_02055 0.83 +1.2 461,068 + PS417_02055 0.84 -0.2 461,459 - PS417_02060 0.22 -0.6 461,814 + PS417_02060 0.51 -0.6 461,815 - PS417_02060 0.51 -0.7 461,992 + PS417_02060 0.65 -0.3 462,226 + PS417_02060 0.84 -0.9 462,328 + -0.6 462,328 + +0.2 462,328 + -0.4 462,328 + +3.1 462,329 - +0.2 462,329 - +0.1 462,329 - -0.4 462,421 + -0.3 462,421 + -0.4 462,466 + -1.5 462,467 - +0.2 462,467 - +1.4 462,504 + -1.3 462,562 - +1.9 462,625 + -1.7 463,063 + PS417_02065 0.26 +0.9 463,063 + PS417_02065 0.26 -0.1 463,090 - PS417_02065 0.27 +0.7
Or see this region's nucleotide sequence