Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18050 and PS417_18055 overlap by 4 nucleotides PS417_18055 and PS417_18060 overlap by 4 nucleotides PS417_18060 and PS417_18065 overlap by 4 nucleotides PS417_18065 and PS417_18070 overlap by 4 nucleotides PS417_18070 and PS417_18075 are separated by 7 nucleotides
PS417_18050: PS417_18050 - general secretion pathway protein GspL, at 3,933,520 to 3,934,512
_18050
PS417_18055: PS417_18055 - general secretion pathway protein GspK, at 3,934,509 to 3,935,210
_18055
PS417_18060: PS417_18060 - general secretion pathway protein GspJ, at 3,935,207 to 3,935,776
_18060
PS417_18065: PS417_18065 - general secretion pathway protein GspI, at 3,935,773 to 3,936,105
_18065
PS417_18070: PS417_18070 - general secretion pathway protein GspH, at 3,936,102 to 3,936,527
_18070
PS417_18075: PS417_18075 - general secretion pathway protein GspG, at 3,936,535 to 3,936,924
_18075
Position (kb) 3935
3936 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3934.566 kb on + strand at 3934.765 kb on + strand, within PS417_18055 at 3934.940 kb on - strand, within PS417_18055 at 3935.081 kb on + strand at 3935.646 kb on + strand, within PS417_18060 at 3935.646 kb on + strand, within PS417_18060 at 3935.730 kb on - strand at 3935.752 kb on + strand at 3936.214 kb on + strand, within PS417_18070 at 3936.215 kb on - strand at 3936.510 kb on - strand at 3936.596 kb on + strand, within PS417_18075 at 3936.597 kb on - strand, within PS417_18075 at 3936.686 kb on + strand, within PS417_18075 at 3936.687 kb on - strand, within PS417_18075 at 3936.715 kb on + strand, within PS417_18075 at 3936.716 kb on - strand, within PS417_18075 at 3936.716 kb on - strand, within PS417_18075
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,934,566 + -3.2 3,934,765 + PS417_18055 0.36 -3.3 3,934,940 - PS417_18055 0.61 -0.9 3,935,081 + -0.2 3,935,646 + PS417_18060 0.77 -0.5 3,935,646 + PS417_18060 0.77 +0.3 3,935,730 - +0.2 3,935,752 + +1.2 3,936,214 + PS417_18070 0.26 +0.5 3,936,215 - +0.8 3,936,510 - +1.8 3,936,596 + PS417_18075 0.16 -0.4 3,936,597 - PS417_18075 0.16 -0.6 3,936,686 + PS417_18075 0.39 -0.4 3,936,687 - PS417_18075 0.39 -0.2 3,936,715 + PS417_18075 0.46 +0.1 3,936,716 - PS417_18075 0.46 -0.4 3,936,716 - PS417_18075 0.46 -0.5
Or see this region's nucleotide sequence