Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17825 and PS417_17830 are separated by 128 nucleotides PS417_17830 and PS417_17835 overlap by 4 nucleotides PS417_17835 and PS417_17840 are separated by 5 nucleotides
PS417_17825: PS417_17825 - AraC family transcriptional regulator, at 3,892,884 to 3,893,744
_17825
PS417_17830: PS417_17830 - 4-hydroxyphenylacetate isomerase, at 3,893,873 to 3,894,538
_17830
PS417_17835: PS417_17835 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 3,894,535 to 3,895,305
_17835
PS417_17840: PS417_17840 - betaine-aldehyde dehydrogenase, at 3,895,311 to 3,896,771
_17840
Position (kb)
3894
3895
3896 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3893.548 kb on + strand, within PS417_17825 at 3893.549 kb on - strand, within PS417_17825 at 3893.566 kb on + strand, within PS417_17825 at 3893.593 kb on + strand, within PS417_17825 at 3893.594 kb on - strand, within PS417_17825 at 3893.594 kb on - strand, within PS417_17825 at 3893.674 kb on + strand at 3893.674 kb on + strand at 3893.696 kb on - strand at 3893.696 kb on - strand at 3893.696 kb on - strand at 3893.707 kb on + strand at 3893.822 kb on - strand at 3893.842 kb on - strand at 3893.930 kb on + strand at 3893.930 kb on + strand at 3894.011 kb on - strand, within PS417_17830 at 3894.161 kb on + strand, within PS417_17830 at 3894.162 kb on - strand, within PS417_17830 at 3894.200 kb on + strand, within PS417_17830 at 3894.201 kb on - strand, within PS417_17830 at 3894.201 kb on - strand, within PS417_17830 at 3894.515 kb on - strand at 3894.766 kb on + strand, within PS417_17835 at 3894.766 kb on + strand, within PS417_17835 at 3894.767 kb on - strand, within PS417_17835 at 3894.821 kb on - strand, within PS417_17835 at 3894.821 kb on - strand, within PS417_17835 at 3894.837 kb on - strand, within PS417_17835 at 3895.130 kb on - strand at 3895.368 kb on + strand at 3895.369 kb on - strand at 3895.369 kb on - strand at 3895.369 kb on - strand at 3895.369 kb on - strand at 3895.698 kb on + strand, within PS417_17840 at 3895.699 kb on - strand, within PS417_17840
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,893,548 + PS417_17825 0.77 -0.9 3,893,549 - PS417_17825 0.77 +0.0 3,893,566 + PS417_17825 0.79 +0.2 3,893,593 + PS417_17825 0.82 -0.2 3,893,594 - PS417_17825 0.82 +0.9 3,893,594 - PS417_17825 0.82 -0.7 3,893,674 + +0.1 3,893,674 + -1.5 3,893,696 - +0.3 3,893,696 - +0.5 3,893,696 - +0.6 3,893,707 + -0.9 3,893,822 - +1.0 3,893,842 - -0.2 3,893,930 + +0.5 3,893,930 + +0.3 3,894,011 - PS417_17830 0.21 -0.3 3,894,161 + PS417_17830 0.43 -0.9 3,894,162 - PS417_17830 0.43 -0.3 3,894,200 + PS417_17830 0.49 -0.1 3,894,201 - PS417_17830 0.49 -1.2 3,894,201 - PS417_17830 0.49 +0.3 3,894,515 - +0.5 3,894,766 + PS417_17835 0.30 +1.2 3,894,766 + PS417_17835 0.30 +0.1 3,894,767 - PS417_17835 0.30 +3.1 3,894,821 - PS417_17835 0.37 +0.2 3,894,821 - PS417_17835 0.37 +1.1 3,894,837 - PS417_17835 0.39 -0.4 3,895,130 - +0.6 3,895,368 + -0.1 3,895,369 - -0.4 3,895,369 - +0.6 3,895,369 - +0.0 3,895,369 - -1.6 3,895,698 + PS417_17840 0.26 +0.4 3,895,699 - PS417_17840 0.27 -0.3
Or see this region's nucleotide sequence