Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01760 and PS417_01765 are separated by 12 nucleotides PS417_01765 and PS417_01770 are separated by 129 nucleotides
PS417_01760: PS417_01760 - imidazolonepropionase, at 391,604 to 392,809
_01760
PS417_01765: PS417_01765 - N-formylglutamate amidohydrolase, at 392,822 to 393,622
_01765
PS417_01770: PS417_01770 - glycine/betaine ABC transporter substrate-binding protein, at 393,752 to 394,687
_01770
Position (kb)
392
393
394 Strain fitness (log2 ratio)
-2
-1
0
1 at 391.866 kb on - strand, within PS417_01760 at 391.998 kb on + strand at 392.098 kb on + strand, within PS417_01760 at 392.098 kb on + strand, within PS417_01760 at 392.153 kb on + strand, within PS417_01760 at 392.460 kb on - strand, within PS417_01760 at 392.747 kb on + strand at 392.817 kb on + strand at 392.817 kb on + strand at 392.817 kb on + strand at 392.818 kb on - strand at 392.818 kb on - strand at 392.818 kb on - strand at 392.818 kb on - strand at 393.687 kb on - strand at 393.721 kb on + strand at 393.722 kb on - strand at 393.726 kb on + strand at 393.726 kb on + strand at 393.982 kb on + strand, within PS417_01770 at 394.109 kb on + strand, within PS417_01770 at 394.181 kb on + strand, within PS417_01770 at 394.181 kb on + strand, within PS417_01770 at 394.407 kb on + strand, within PS417_01770 at 394.407 kb on + strand, within PS417_01770 at 394.408 kb on - strand, within PS417_01770
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 391,866 - PS417_01760 0.22 +1.2 391,998 + -1.7 392,098 + PS417_01760 0.41 +1.3 392,098 + PS417_01760 0.41 +1.2 392,153 + PS417_01760 0.46 -2.1 392,460 - PS417_01760 0.71 -0.2 392,747 + +0.2 392,817 + +0.5 392,817 + -0.2 392,817 + -1.5 392,818 - +0.8 392,818 - +1.4 392,818 - +1.1 392,818 - +1.6 393,687 - +0.8 393,721 + -0.8 393,722 - -0.4 393,726 + -0.3 393,726 + -0.1 393,982 + PS417_01770 0.25 -0.2 394,109 + PS417_01770 0.38 +0.2 394,181 + PS417_01770 0.46 -0.2 394,181 + PS417_01770 0.46 -0.6 394,407 + PS417_01770 0.70 -1.9 394,407 + PS417_01770 0.70 -1.2 394,408 - PS417_01770 0.70 -0.2
Or see this region's nucleotide sequence