Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16285 and PS417_16290 are separated by 800 nucleotides PS417_16290 and PS417_16295 are separated by 179 nucleotides PS417_16295 and PS417_16305 are separated by 398 nucleotides
PS417_16285: PS417_16285 - diguanylate cyclase, at 3,567,634 to 3,568,491
_16285
PS417_16290: PS417_16290 - hypothetical protein, at 3,569,292 to 3,569,675
_16290
PS417_16295: PS417_16295 - hypothetical protein, at 3,569,855 to 3,570,187
_16295
PS417_16305: PS417_16305 - tRNA-Ser, at 3,570,586 to 3,570,676
_16305
Position (kb)
3569
3570 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3568.325 kb on + strand, within PS417_16285 at 3568.325 kb on + strand, within PS417_16285 at 3568.326 kb on - strand, within PS417_16285 at 3568.338 kb on - strand, within PS417_16285 at 3568.431 kb on + strand at 3568.463 kb on - strand at 3568.463 kb on - strand at 3568.489 kb on + strand at 3568.489 kb on + strand at 3568.489 kb on + strand at 3568.518 kb on - strand at 3568.636 kb on + strand at 3568.637 kb on - strand at 3568.662 kb on + strand at 3568.663 kb on - strand at 3568.776 kb on - strand at 3568.848 kb on + strand at 3568.866 kb on - strand at 3568.866 kb on - strand at 3568.908 kb on - strand at 3568.909 kb on - strand at 3568.943 kb on + strand at 3568.954 kb on + strand at 3569.013 kb on - strand at 3569.074 kb on + strand at 3569.090 kb on + strand at 3569.090 kb on + strand at 3569.091 kb on - strand at 3569.125 kb on - strand at 3569.125 kb on - strand at 3569.304 kb on + strand at 3569.307 kb on + strand at 3569.307 kb on + strand at 3569.308 kb on - strand at 3569.336 kb on + strand, within PS417_16290 at 3569.387 kb on + strand, within PS417_16290 at 3569.404 kb on - strand, within PS417_16290 at 3569.404 kb on - strand, within PS417_16290 at 3569.519 kb on + strand at 3569.519 kb on + strand, within PS417_16290 at 3569.520 kb on - strand, within PS417_16290 at 3569.520 kb on - strand, within PS417_16290 at 3569.535 kb on - strand, within PS417_16290 at 3569.535 kb on - strand, within PS417_16290 at 3569.535 kb on - strand, within PS417_16290 at 3569.598 kb on + strand, within PS417_16290 at 3569.598 kb on + strand, within PS417_16290 at 3569.659 kb on + strand at 3569.668 kb on - strand at 3569.674 kb on - strand at 3569.724 kb on + strand at 3569.774 kb on + strand at 3569.853 kb on + strand at 3569.856 kb on + strand at 3569.947 kb on + strand, within PS417_16295 at 3569.947 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.949 kb on + strand, within PS417_16295 at 3569.989 kb on - strand, within PS417_16295 at 3570.040 kb on - strand at 3570.040 kb on - strand, within PS417_16295 at 3570.063 kb on - strand, within PS417_16295 at 3570.181 kb on - strand at 3570.209 kb on - strand at 3570.312 kb on - strand at 3570.318 kb on - strand at 3570.365 kb on - strand at 3570.365 kb on - strand at 3570.383 kb on - strand at 3570.425 kb on - strand at 3570.484 kb on + strand at 3570.535 kb on + strand at 3570.535 kb on + strand at 3570.536 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,568,325 + PS417_16285 0.81 +1.9 3,568,325 + PS417_16285 0.81 +1.1 3,568,326 - PS417_16285 0.81 +0.0 3,568,338 - PS417_16285 0.82 -0.4 3,568,431 + -0.9 3,568,463 - -0.7 3,568,463 - -0.1 3,568,489 + -0.1 3,568,489 + +0.4 3,568,489 + -0.3 3,568,518 - -0.1 3,568,636 + +0.2 3,568,637 - +1.1 3,568,662 + -0.5 3,568,663 - -0.1 3,568,776 - +1.3 3,568,848 + +0.1 3,568,866 - +0.1 3,568,866 - +1.4 3,568,908 - -1.9 3,568,909 - +2.7 3,568,943 + -0.3 3,568,954 + -0.5 3,569,013 - +1.2 3,569,074 + +1.1 3,569,090 + +0.6 3,569,090 + +0.9 3,569,091 - -0.9 3,569,125 - -0.3 3,569,125 - -0.2 3,569,304 + -0.6 3,569,307 + -0.2 3,569,307 + +0.2 3,569,308 - -0.5 3,569,336 + PS417_16290 0.11 +0.2 3,569,387 + PS417_16290 0.25 +0.9 3,569,404 - PS417_16290 0.29 -0.6 3,569,404 - PS417_16290 0.29 -0.1 3,569,519 + +3.3 3,569,519 + PS417_16290 0.59 -0.5 3,569,520 - PS417_16290 0.59 -0.4 3,569,520 - PS417_16290 0.59 -0.9 3,569,535 - PS417_16290 0.63 -0.1 3,569,535 - PS417_16290 0.63 -0.2 3,569,535 - PS417_16290 0.63 -0.7 3,569,598 + PS417_16290 0.80 -0.9 3,569,598 + PS417_16290 0.80 -0.3 3,569,659 + -0.3 3,569,668 - +1.0 3,569,674 - +0.9 3,569,724 + +0.8 3,569,774 + -1.6 3,569,853 + +2.3 3,569,856 + -0.4 3,569,947 + PS417_16295 0.28 -0.0 3,569,947 + PS417_16295 0.28 +1.5 3,569,949 + PS417_16295 0.28 +1.2 3,569,949 + PS417_16295 0.28 -0.6 3,569,949 + PS417_16295 0.28 +0.6 3,569,949 + PS417_16295 0.28 +2.4 3,569,949 + PS417_16295 0.28 -0.3 3,569,949 + PS417_16295 0.28 +1.3 3,569,989 - PS417_16295 0.40 -0.7 3,570,040 - +0.7 3,570,040 - PS417_16295 0.56 +1.3 3,570,063 - PS417_16295 0.62 -0.8 3,570,181 - +0.9 3,570,209 - -0.2 3,570,312 - -0.4 3,570,318 - +1.0 3,570,365 - +0.1 3,570,365 - +0.2 3,570,383 - +0.5 3,570,425 - +0.3 3,570,484 + +0.7 3,570,535 + +0.7 3,570,535 + -1.1 3,570,536 - -0.1
Or see this region's nucleotide sequence