Strain Fitness in Pseudomonas simiae WCS417 around PS417_14505

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_14495 and PS417_14500 are separated by 24 nucleotidesPS417_14500 and PS417_14505 are separated by 119 nucleotidesPS417_14505 and PS417_14510 are separated by 1 nucleotidesPS417_14510 and PS417_14515 overlap by 4 nucleotides PS417_14495: PS417_14495 - maltooligosyl trehalose synthase, at 3,168,243 to 3,170,981 _14495 PS417_14500: PS417_14500 - hypothetical protein, at 3,171,006 to 3,171,278 _14500 PS417_14505: PS417_14505 - glycogen debranching protein, at 3,171,398 to 3,173,557 _14505 PS417_14510: PS417_14510 - acyl-CoA dehydrogenase, at 3,173,559 to 3,174,557 _14510 PS417_14515: PS417_14515 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,174,554 to 3,175,312 _14515 Position (kb) 3171 3172 3173 3174Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3170.457 kb on + strand, within PS417_14495at 3170.668 kb on - strand, within PS417_14495at 3171.012 kb on - strandat 3171.028 kb on + strandat 3171.653 kb on + strand, within PS417_14505at 3172.118 kb on + strand, within PS417_14505at 3172.119 kb on - strandat 3172.250 kb on + strand, within PS417_14505at 3172.319 kb on + strand, within PS417_14505at 3172.319 kb on + strand, within PS417_14505at 3172.352 kb on + strand, within PS417_14505at 3172.352 kb on + strand, within PS417_14505at 3172.353 kb on - strand, within PS417_14505at 3172.353 kb on - strand, within PS417_14505at 3173.195 kb on + strand, within PS417_14505at 3173.195 kb on + strand, within PS417_14505at 3174.471 kb on + strandat 3174.471 kb on + strandat 3174.471 kb on + strandat 3174.472 kb on - strandat 3174.472 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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3,170,457 + PS417_14495 0.81 -0.2
3,170,668 - PS417_14495 0.89 +0.7
3,171,012 - +1.3
3,171,028 + -0.4
3,171,653 + PS417_14505 0.12 -0.0
3,172,118 + PS417_14505 0.33 +0.6
3,172,119 - -1.3
3,172,250 + PS417_14505 0.39 +2.0
3,172,319 + PS417_14505 0.43 +1.2
3,172,319 + PS417_14505 0.43 +0.8
3,172,352 + PS417_14505 0.44 -0.0
3,172,352 + PS417_14505 0.44 -1.9
3,172,353 - PS417_14505 0.44 +3.2
3,172,353 - PS417_14505 0.44 -0.8
3,173,195 + PS417_14505 0.83 -0.8
3,173,195 + PS417_14505 0.83 -0.9
3,174,471 + -0.3
3,174,471 + -0.3
3,174,471 + -0.7
3,174,472 - -0.1
3,174,472 - -0.2

Or see this region's nucleotide sequence