Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14495 and PS417_14500 are separated by 24 nucleotides PS417_14500 and PS417_14505 are separated by 119 nucleotides PS417_14505 and PS417_14510 are separated by 1 nucleotides PS417_14510 and PS417_14515 overlap by 4 nucleotides
PS417_14495: PS417_14495 - maltooligosyl trehalose synthase, at 3,168,243 to 3,170,981
_14495
PS417_14500: PS417_14500 - hypothetical protein, at 3,171,006 to 3,171,278
_14500
PS417_14505: PS417_14505 - glycogen debranching protein, at 3,171,398 to 3,173,557
_14505
PS417_14510: PS417_14510 - acyl-CoA dehydrogenase, at 3,173,559 to 3,174,557
_14510
PS417_14515: PS417_14515 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,174,554 to 3,175,312
_14515
Position (kb)
3171
3172
3173
3174 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3170.457 kb on + strand, within PS417_14495 at 3170.668 kb on - strand, within PS417_14495 at 3171.012 kb on - strand at 3171.028 kb on + strand at 3171.653 kb on + strand, within PS417_14505 at 3172.118 kb on + strand, within PS417_14505 at 3172.119 kb on - strand at 3172.250 kb on + strand, within PS417_14505 at 3172.319 kb on + strand, within PS417_14505 at 3172.319 kb on + strand, within PS417_14505 at 3172.352 kb on + strand, within PS417_14505 at 3172.352 kb on + strand, within PS417_14505 at 3172.353 kb on - strand, within PS417_14505 at 3172.353 kb on - strand, within PS417_14505 at 3173.195 kb on + strand, within PS417_14505 at 3173.195 kb on + strand, within PS417_14505 at 3174.471 kb on + strand at 3174.471 kb on + strand at 3174.471 kb on + strand at 3174.472 kb on - strand at 3174.472 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,170,457 + PS417_14495 0.81 -0.2 3,170,668 - PS417_14495 0.89 +0.7 3,171,012 - +1.3 3,171,028 + -0.4 3,171,653 + PS417_14505 0.12 -0.0 3,172,118 + PS417_14505 0.33 +0.6 3,172,119 - -1.3 3,172,250 + PS417_14505 0.39 +2.0 3,172,319 + PS417_14505 0.43 +1.2 3,172,319 + PS417_14505 0.43 +0.8 3,172,352 + PS417_14505 0.44 -0.0 3,172,352 + PS417_14505 0.44 -1.9 3,172,353 - PS417_14505 0.44 +3.2 3,172,353 - PS417_14505 0.44 -0.8 3,173,195 + PS417_14505 0.83 -0.8 3,173,195 + PS417_14505 0.83 -0.9 3,174,471 + -0.3 3,174,471 + -0.3 3,174,471 + -0.7 3,174,472 - -0.1 3,174,472 - -0.2
Or see this region's nucleotide sequence