Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00810 and PS417_00815 overlap by 4 nucleotides PS417_00815 and PS417_00820 are separated by 291 nucleotides PS417_00820 and PS417_00825 are separated by 890 nucleotides
PS417_00810: PS417_00810 - RNA polymerase sigma factor, at 179,799 to 180,371
_00810
PS417_00815: PS417_00815 - hypothetical protein, at 180,368 to 180,961
_00815
PS417_00820: PS417_00820 - hypothetical protein, at 181,253 to 181,504
_00820
PS417_00825: PS417_00825 - recombinase RecF, at 182,395 to 183,564
_00825
Position (kb)
181
182 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 181.012 kb on + strand at 181.013 kb on - strand at 181.013 kb on - strand at 181.097 kb on + strand at 181.097 kb on + strand at 181.097 kb on + strand at 181.098 kb on - strand at 181.110 kb on - strand at 181.255 kb on - strand at 181.255 kb on - strand at 181.278 kb on - strand at 181.294 kb on - strand, within PS417_00820 at 181.307 kb on + strand, within PS417_00820 at 181.307 kb on + strand, within PS417_00820 at 181.307 kb on + strand, within PS417_00820 at 181.308 kb on - strand, within PS417_00820 at 181.308 kb on - strand, within PS417_00820 at 181.308 kb on - strand, within PS417_00820 at 181.308 kb on - strand at 181.308 kb on - strand, within PS417_00820 at 181.350 kb on - strand, within PS417_00820 at 181.361 kb on + strand, within PS417_00820 at 181.372 kb on + strand, within PS417_00820 at 181.373 kb on - strand, within PS417_00820 at 181.461 kb on - strand, within PS417_00820 at 181.478 kb on + strand, within PS417_00820 at 181.498 kb on + strand at 181.499 kb on - strand at 181.562 kb on + strand at 181.562 kb on + strand at 181.593 kb on + strand at 181.745 kb on + strand at 181.785 kb on + strand at 181.785 kb on + strand at 181.785 kb on + strand at 181.786 kb on - strand at 181.887 kb on + strand at 181.942 kb on + strand at 181.942 kb on + strand at 181.942 kb on + strand at 181.943 kb on - strand at 181.943 kb on - strand at 181.943 kb on - strand at 182.021 kb on + strand at 182.095 kb on - strand at 182.095 kb on - strand at 182.127 kb on + strand at 182.145 kb on + strand at 182.154 kb on - strand at 182.154 kb on - strand at 182.154 kb on - strand at 182.230 kb on - strand at 182.230 kb on - strand at 182.230 kb on - strand at 182.230 kb on - strand at 182.306 kb on + strand at 182.306 kb on + strand at 182.328 kb on - strand at 182.362 kb on + strand at 182.362 kb on + strand at 182.363 kb on - strand at 182.363 kb on - strand at 182.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 181,012 + -0.3 181,013 - -2.2 181,013 - -0.7 181,097 + +0.9 181,097 + +0.5 181,097 + -2.4 181,098 - +0.2 181,110 - +2.2 181,255 - +0.5 181,255 - -0.7 181,278 - +1.9 181,294 - PS417_00820 0.16 +1.6 181,307 + PS417_00820 0.21 +0.4 181,307 + PS417_00820 0.21 +0.1 181,307 + PS417_00820 0.21 +0.7 181,308 - PS417_00820 0.22 +1.1 181,308 - PS417_00820 0.22 -2.1 181,308 - PS417_00820 0.22 -0.0 181,308 - +1.5 181,308 - PS417_00820 0.22 +0.7 181,350 - PS417_00820 0.38 +3.3 181,361 + PS417_00820 0.43 +0.6 181,372 + PS417_00820 0.47 -0.2 181,373 - PS417_00820 0.48 +0.3 181,461 - PS417_00820 0.83 +0.0 181,478 + PS417_00820 0.89 -0.2 181,498 + +2.8 181,499 - -0.0 181,562 + +0.5 181,562 + -0.3 181,593 + -0.0 181,745 + +0.3 181,785 + +0.5 181,785 + -0.9 181,785 + -0.5 181,786 - +0.4 181,887 + -0.9 181,942 + +0.2 181,942 + -0.9 181,942 + -0.1 181,943 - -0.1 181,943 - +0.5 181,943 - -0.8 182,021 + +0.4 182,095 - -0.8 182,095 - +0.3 182,127 + -0.3 182,145 + +0.5 182,154 - +0.7 182,154 - -1.1 182,154 - +0.4 182,230 - +0.0 182,230 - -0.7 182,230 - -1.3 182,230 - -0.4 182,306 + -1.2 182,306 + +0.0 182,328 - -0.2 182,362 + -0.4 182,362 + -0.4 182,363 - -0.5 182,363 - -0.1 182,389 - +0.9
Or see this region's nucleotide sequence