Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07180 and PS417_07185 are separated by 83 nucleotides PS417_07185 and PS417_07190 are separated by 352 nucleotides PS417_07190 and PS417_07195 are separated by 282 nucleotides PS417_07195 and PS417_07200 are separated by 50 nucleotides
PS417_07180: PS417_07180 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 1,585,966 to 1,586,679
_07180
PS417_07185: PS417_07185 - tRNA-Ser, at 1,586,763 to 1,586,852
_07185
PS417_07190: PS417_07190 - MFS transporter, at 1,587,205 to 1,588,431
_07190
PS417_07195: PS417_07195 - hypothetical protein, at 1,588,714 to 1,588,968
_07195
PS417_07200: PS417_07200 - LysR family transcriptional regulator, at 1,589,019 to 1,589,984
_07200
Position (kb)
1587
1588
1589 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1586.698 kb on - strand at 1586.950 kb on + strand at 1586.962 kb on + strand at 1586.962 kb on + strand at 1586.963 kb on - strand at 1586.963 kb on - strand at 1587.194 kb on + strand at 1587.194 kb on + strand at 1587.194 kb on + strand at 1587.195 kb on - strand at 1587.228 kb on + strand at 1587.228 kb on + strand at 1587.229 kb on - strand at 1587.229 kb on - strand at 1587.229 kb on - strand at 1587.361 kb on - strand, within PS417_07190 at 1587.566 kb on - strand, within PS417_07190 at 1587.573 kb on - strand, within PS417_07190 at 1587.704 kb on + strand, within PS417_07190 at 1587.705 kb on - strand, within PS417_07190 at 1587.916 kb on + strand, within PS417_07190 at 1587.943 kb on + strand at 1588.389 kb on + strand at 1588.389 kb on + strand at 1588.389 kb on + strand at 1588.389 kb on + strand at 1588.390 kb on - strand at 1588.390 kb on - strand at 1588.390 kb on - strand at 1588.486 kb on - strand at 1588.486 kb on - strand at 1588.498 kb on + strand at 1588.541 kb on + strand at 1588.541 kb on + strand at 1588.541 kb on + strand at 1588.542 kb on - strand at 1588.624 kb on + strand at 1588.657 kb on + strand at 1588.660 kb on + strand at 1588.660 kb on + strand at 1588.748 kb on + strand, within PS417_07195 at 1588.748 kb on + strand, within PS417_07195 at 1588.749 kb on - strand, within PS417_07195 at 1588.900 kb on + strand, within PS417_07195 at 1588.900 kb on + strand, within PS417_07195 at 1588.901 kb on - strand, within PS417_07195 at 1588.901 kb on - strand, within PS417_07195 at 1588.970 kb on + strand at 1589.031 kb on - strand at 1589.078 kb on + strand at 1589.124 kb on + strand, within PS417_07200 at 1589.124 kb on + strand, within PS417_07200 at 1589.124 kb on + strand, within PS417_07200 at 1589.125 kb on - strand, within PS417_07200 at 1589.125 kb on - strand, within PS417_07200 at 1589.125 kb on - strand, within PS417_07200 at 1589.125 kb on - strand, within PS417_07200
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,586,698 - -1.0 1,586,950 + +0.1 1,586,962 + +0.4 1,586,962 + +0.0 1,586,963 - +0.1 1,586,963 - -2.3 1,587,194 + +0.7 1,587,194 + -0.3 1,587,194 + -0.4 1,587,195 - -0.3 1,587,228 + -0.5 1,587,228 + -2.7 1,587,229 - -0.9 1,587,229 - +0.8 1,587,229 - +0.2 1,587,361 - PS417_07190 0.13 -0.8 1,587,566 - PS417_07190 0.29 -1.5 1,587,573 - PS417_07190 0.30 -1.2 1,587,704 + PS417_07190 0.41 -0.4 1,587,705 - PS417_07190 0.41 -1.9 1,587,916 + PS417_07190 0.58 -0.5 1,587,943 + -0.4 1,588,389 + +0.0 1,588,389 + +0.4 1,588,389 + -0.4 1,588,389 + +0.2 1,588,390 - -0.6 1,588,390 - +0.6 1,588,390 - -0.5 1,588,486 - +0.6 1,588,486 - +0.6 1,588,498 + -1.6 1,588,541 + +1.6 1,588,541 + +1.3 1,588,541 + +0.5 1,588,542 - +4.6 1,588,624 + -0.1 1,588,657 + +0.8 1,588,660 + -0.2 1,588,660 + -0.4 1,588,748 + PS417_07195 0.13 -0.6 1,588,748 + PS417_07195 0.13 +0.5 1,588,749 - PS417_07195 0.14 +2.3 1,588,900 + PS417_07195 0.73 -0.4 1,588,900 + PS417_07195 0.73 +1.7 1,588,901 - PS417_07195 0.73 -1.1 1,588,901 - PS417_07195 0.73 +0.8 1,588,970 + -0.8 1,589,031 - +0.2 1,589,078 + -0.0 1,589,124 + PS417_07200 0.11 -0.8 1,589,124 + PS417_07200 0.11 -0.2 1,589,124 + PS417_07200 0.11 -0.2 1,589,125 - PS417_07200 0.11 -1.1 1,589,125 - PS417_07200 0.11 -0.2 1,589,125 - PS417_07200 0.11 +0.8 1,589,125 - PS417_07200 0.11 +0.2
Or see this region's nucleotide sequence