Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00520 and PS417_00525 are separated by 376 nucleotides PS417_00525 and PS417_00530 overlap by 4 nucleotides PS417_00530 and PS417_00535 are separated by 26 nucleotides PS417_00535 and PS417_00540 are separated by 80 nucleotides
PS417_00520: PS417_00520 - citrate transporter, at 104,240 to 105,547
_00520
PS417_00525: PS417_00525 - hypothetical protein, at 105,924 to 107,885
_00525
PS417_00530: PS417_00530 - hypothetical protein, at 107,882 to 108,202
_00530
PS417_00535: PS417_00535 - Holliday junction resolvase, at 108,229 to 108,537
_00535
PS417_00540: PS417_00540 - hypothetical protein, at 108,618 to 108,938
_00540
Position (kb)
105
106
107
108 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 104.964 kb on + strand, within PS417_00520 at 105.195 kb on + strand, within PS417_00520 at 105.195 kb on + strand, within PS417_00520 at 105.195 kb on + strand, within PS417_00520 at 105.195 kb on + strand, within PS417_00520 at 105.196 kb on - strand, within PS417_00520 at 105.196 kb on - strand, within PS417_00520 at 105.196 kb on - strand, within PS417_00520 at 105.196 kb on - strand, within PS417_00520 at 105.196 kb on - strand, within PS417_00520 at 105.342 kb on + strand, within PS417_00520 at 105.342 kb on + strand, within PS417_00520 at 105.343 kb on - strand, within PS417_00520 at 105.547 kb on + strand at 105.548 kb on - strand at 105.553 kb on + strand at 105.554 kb on - strand at 105.554 kb on - strand at 105.565 kb on - strand at 105.690 kb on - strand at 105.733 kb on + strand at 105.734 kb on - strand at 105.783 kb on + strand at 105.783 kb on + strand at 105.784 kb on - strand at 105.784 kb on - strand at 105.784 kb on - strand at 105.784 kb on - strand at 105.798 kb on + strand at 105.925 kb on + strand at 105.953 kb on + strand at 106.265 kb on - strand, within PS417_00525 at 106.265 kb on - strand, within PS417_00525 at 106.493 kb on - strand, within PS417_00525 at 106.561 kb on - strand, within PS417_00525 at 106.648 kb on - strand, within PS417_00525 at 106.675 kb on + strand, within PS417_00525 at 106.675 kb on + strand, within PS417_00525 at 106.676 kb on - strand, within PS417_00525 at 106.687 kb on + strand, within PS417_00525 at 106.688 kb on - strand, within PS417_00525 at 106.688 kb on - strand, within PS417_00525 at 106.789 kb on + strand, within PS417_00525 at 106.789 kb on + strand, within PS417_00525 at 106.790 kb on - strand, within PS417_00525 at 106.790 kb on - strand, within PS417_00525 at 106.872 kb on + strand, within PS417_00525 at 106.872 kb on + strand, within PS417_00525 at 106.872 kb on + strand, within PS417_00525 at 106.873 kb on - strand, within PS417_00525 at 106.873 kb on - strand, within PS417_00525 at 106.895 kb on - strand, within PS417_00525 at 107.042 kb on - strand, within PS417_00525 at 107.110 kb on + strand, within PS417_00525 at 107.164 kb on + strand, within PS417_00525 at 107.164 kb on + strand, within PS417_00525 at 107.164 kb on + strand, within PS417_00525 at 107.164 kb on + strand, within PS417_00525 at 107.197 kb on + strand, within PS417_00525 at 107.198 kb on - strand, within PS417_00525 at 107.263 kb on + strand at 107.263 kb on + strand, within PS417_00525 at 107.263 kb on + strand, within PS417_00525 at 107.326 kb on + strand, within PS417_00525 at 107.326 kb on + strand, within PS417_00525 at 107.326 kb on + strand, within PS417_00525 at 107.327 kb on - strand, within PS417_00525 at 107.501 kb on - strand, within PS417_00525 at 107.552 kb on + strand, within PS417_00525 at 107.657 kb on - strand, within PS417_00525 at 107.896 kb on + strand at 108.196 kb on + strand at 108.197 kb on - strand at 108.197 kb on - strand at 108.197 kb on - strand at 108.300 kb on - strand, within PS417_00535 at 108.300 kb on - strand, within PS417_00535 at 108.429 kb on - strand, within PS417_00535 at 108.514 kb on + strand at 108.576 kb on - strand at 108.623 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 104,964 + PS417_00520 0.55 -0.3 105,195 + PS417_00520 0.73 -1.1 105,195 + PS417_00520 0.73 -0.1 105,195 + PS417_00520 0.73 -0.5 105,195 + PS417_00520 0.73 -0.5 105,196 - PS417_00520 0.73 +0.9 105,196 - PS417_00520 0.73 -0.3 105,196 - PS417_00520 0.73 -0.1 105,196 - PS417_00520 0.73 -0.1 105,196 - PS417_00520 0.73 +0.5 105,342 + PS417_00520 0.84 -0.0 105,342 + PS417_00520 0.84 +0.3 105,343 - PS417_00520 0.84 -0.0 105,547 + -0.3 105,548 - +0.8 105,553 + -1.3 105,554 - -0.3 105,554 - -0.1 105,565 - +0.5 105,690 - +1.9 105,733 + -0.1 105,734 - -1.1 105,783 + -0.4 105,783 + -0.3 105,784 - -0.1 105,784 - +0.3 105,784 - -0.7 105,784 - -0.7 105,798 + -1.3 105,925 + -1.4 105,953 + -0.4 106,265 - PS417_00525 0.17 -0.5 106,265 - PS417_00525 0.17 +0.6 106,493 - PS417_00525 0.29 +0.1 106,561 - PS417_00525 0.32 -0.8 106,648 - PS417_00525 0.37 -1.4 106,675 + PS417_00525 0.38 -0.7 106,675 + PS417_00525 0.38 -0.4 106,676 - PS417_00525 0.38 -0.9 106,687 + PS417_00525 0.39 -0.3 106,688 - PS417_00525 0.39 -0.5 106,688 - PS417_00525 0.39 +1.5 106,789 + PS417_00525 0.44 -1.4 106,789 + PS417_00525 0.44 +1.5 106,790 - PS417_00525 0.44 -1.6 106,790 - PS417_00525 0.44 -1.1 106,872 + PS417_00525 0.48 -0.4 106,872 + PS417_00525 0.48 +0.8 106,872 + PS417_00525 0.48 -2.2 106,873 - PS417_00525 0.48 +0.3 106,873 - PS417_00525 0.48 -0.5 106,895 - PS417_00525 0.49 +1.1 107,042 - PS417_00525 0.57 -1.6 107,110 + PS417_00525 0.60 -2.4 107,164 + PS417_00525 0.63 -0.6 107,164 + PS417_00525 0.63 -1.7 107,164 + PS417_00525 0.63 +0.7 107,164 + PS417_00525 0.63 +0.2 107,197 + PS417_00525 0.65 +0.4 107,198 - PS417_00525 0.65 -0.4 107,263 + +3.1 107,263 + PS417_00525 0.68 -1.0 107,263 + PS417_00525 0.68 -0.7 107,326 + PS417_00525 0.71 -1.8 107,326 + PS417_00525 0.71 -0.3 107,326 + PS417_00525 0.71 -1.3 107,327 - PS417_00525 0.72 -1.2 107,501 - PS417_00525 0.80 -1.8 107,552 + PS417_00525 0.83 -1.7 107,657 - PS417_00525 0.88 +1.5 107,896 + -1.1 108,196 + -0.8 108,197 - +0.1 108,197 - -0.4 108,197 - +0.4 108,300 - PS417_00535 0.23 -0.5 108,300 - PS417_00535 0.23 +0.5 108,429 - PS417_00535 0.65 -0.6 108,514 + -0.9 108,576 - -0.5 108,623 + +1.1
Or see this region's nucleotide sequence