Strain Fitness in Pseudomonas simiae WCS417 around PS417_04525

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_04515 and PS417_04520 are separated by 134 nucleotidesPS417_04520 and PS417_04525 are separated by 260 nucleotidesPS417_04525 and PS417_04530 are separated by 30 nucleotides PS417_04515: PS417_04515 - peptidase M48, at 997,720 to 998,538 _04515 PS417_04520: PS417_04520 - membrane protein, at 998,673 to 999,257 _04520 PS417_04525: PS417_04525 - 16S rRNA methyltransferase, at 999,518 to 1,000,516 _04525 PS417_04530: PS417_04530 - glycerate dehydrogenase, at 1,000,547 to 1,001,512 _04530 Position (kb) 999 1000 1001Strain fitness (log2 ratio) -2 -1 0 1 2at 998.543 kb on - strandat 998.562 kb on - strandat 998.608 kb on - strandat 998.608 kb on - strandat 998.674 kb on + strandat 998.674 kb on + strandat 998.675 kb on - strandat 998.675 kb on - strandat 998.704 kb on + strandat 998.705 kb on - strandat 998.705 kb on - strandat 998.705 kb on - strandat 999.262 kb on + strandat 999.263 kb on - strandat 999.335 kb on + strandat 999.335 kb on + strandat 999.336 kb on - strandat 999.405 kb on + strandat 999.405 kb on + strandat 999.405 kb on + strandat 999.405 kb on + strandat 999.406 kb on - strandat 999.406 kb on - strandat 999.406 kb on - strandat 999.406 kb on - strandat 999.447 kb on + strandat 999.448 kb on - strandat 999.735 kb on - strand, within PS417_04525at 999.854 kb on + strand, within PS417_04525at 999.854 kb on + strand, within PS417_04525at 999.855 kb on - strand, within PS417_04525at 999.855 kb on - strand, within PS417_04525at 999.855 kb on - strand, within PS417_04525at 999.855 kb on - strand, within PS417_04525at 999.855 kb on - strand, within PS417_04525at 1000.371 kb on - strand, within PS417_04525at 1000.496 kb on + strandat 1000.497 kb on - strandat 1000.497 kb on - strandat 1000.715 kb on + strand, within PS417_04530at 1000.715 kb on + strand, within PS417_04530at 1000.715 kb on + strand, within PS417_04530at 1000.724 kb on + strand, within PS417_04530at 1001.140 kb on - strand, within PS417_04530

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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998,543 - +0.5
998,562 - +0.8
998,608 - -0.3
998,608 - -1.0
998,674 + +0.0
998,674 + -0.1
998,675 - -0.0
998,675 - -1.0
998,704 + +0.6
998,705 - -1.2
998,705 - -0.7
998,705 - +0.5
999,262 + +0.2
999,263 - +1.5
999,335 + -0.8
999,335 + -2.4
999,336 - +0.5
999,405 + -1.2
999,405 + -0.8
999,405 + -1.3
999,405 + +2.7
999,406 - -0.9
999,406 - -0.0
999,406 - +1.5
999,406 - -0.2
999,447 + +0.7
999,448 - -0.1
999,735 - PS417_04525 0.22 -1.2
999,854 + PS417_04525 0.34 -0.2
999,854 + PS417_04525 0.34 -1.2
999,855 - PS417_04525 0.34 -0.9
999,855 - PS417_04525 0.34 -0.1
999,855 - PS417_04525 0.34 -0.7
999,855 - PS417_04525 0.34 -1.4
999,855 - PS417_04525 0.34 -0.7
1,000,371 - PS417_04525 0.85 +0.9
1,000,496 + -1.4
1,000,497 - +1.0
1,000,497 - +0.2
1,000,715 + PS417_04530 0.17 -0.2
1,000,715 + PS417_04530 0.17 -1.7
1,000,715 + PS417_04530 0.17 -0.1
1,000,724 + PS417_04530 0.18 +1.4
1,001,140 - PS417_04530 0.61 -0.5

Or see this region's nucleotide sequence