Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27020 and PS417_27025 are separated by 120 nucleotides PS417_27025 and PS417_27030 are separated by 243 nucleotides PS417_27030 and PS417_27035 are separated by 21 nucleotides
PS417_27020: PS417_27020 - DNA-binding protein, at 5,834,798 to 5,835,712
_27020
PS417_27025: PS417_27025 - DNA-binding protein, at 5,835,833 to 5,836,759
_27025
PS417_27030: PS417_27030 - hypothetical protein, at 5,837,003 to 5,837,668
_27030
PS417_27035: PS417_27035 - FAD-linked oxidase, at 5,837,690 to 5,839,084
_27035
Position (kb) 5835
5836
5837 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5835.024 kb on + strand, within PS417_27020 at 5835.096 kb on + strand, within PS417_27020 at 5835.138 kb on + strand, within PS417_27020 at 5835.139 kb on - strand, within PS417_27020 at 5835.139 kb on - strand, within PS417_27020 at 5835.195 kb on + strand, within PS417_27020 at 5835.336 kb on + strand, within PS417_27020 at 5835.336 kb on + strand, within PS417_27020 at 5835.337 kb on - strand, within PS417_27020 at 5835.395 kb on - strand, within PS417_27020 at 5835.448 kb on + strand, within PS417_27020 at 5835.483 kb on + strand, within PS417_27020 at 5835.483 kb on + strand, within PS417_27020 at 5835.483 kb on + strand, within PS417_27020 at 5835.484 kb on - strand, within PS417_27020 at 5835.522 kb on - strand, within PS417_27020 at 5835.746 kb on + strand at 5835.747 kb on - strand at 5835.766 kb on - strand at 5835.766 kb on - strand at 5835.772 kb on - strand at 5835.772 kb on - strand at 5835.823 kb on - strand at 5835.834 kb on + strand at 5835.882 kb on + strand at 5835.882 kb on + strand at 5835.883 kb on - strand at 5835.883 kb on - strand at 5835.883 kb on - strand at 5836.150 kb on + strand, within PS417_27025 at 5836.257 kb on + strand, within PS417_27025 at 5836.257 kb on + strand, within PS417_27025 at 5836.257 kb on + strand, within PS417_27025 at 5836.258 kb on - strand, within PS417_27025 at 5836.258 kb on - strand, within PS417_27025 at 5836.258 kb on - strand, within PS417_27025 at 5836.494 kb on + strand, within PS417_27025 at 5836.494 kb on + strand, within PS417_27025 at 5836.495 kb on - strand, within PS417_27025 at 5836.561 kb on - strand, within PS417_27025 at 5836.561 kb on - strand, within PS417_27025 at 5836.575 kb on - strand, within PS417_27025 at 5836.787 kb on + strand at 5836.788 kb on - strand at 5836.890 kb on - strand at 5837.594 kb on - strand, within PS417_27030 at 5837.649 kb on + strand at 5837.649 kb on + strand at 5837.649 kb on + strand at 5837.649 kb on + strand at 5837.649 kb on + strand at 5837.649 kb on + strand at 5837.649 kb on + strand at 5837.650 kb on - strand at 5837.650 kb on - strand at 5837.662 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,835,024 + PS417_27020 0.25 +1.0 5,835,096 + PS417_27020 0.33 +0.3 5,835,138 + PS417_27020 0.37 -0.3 5,835,139 - PS417_27020 0.37 -0.2 5,835,139 - PS417_27020 0.37 -0.2 5,835,195 + PS417_27020 0.43 +0.6 5,835,336 + PS417_27020 0.59 +0.6 5,835,336 + PS417_27020 0.59 +0.1 5,835,337 - PS417_27020 0.59 +0.9 5,835,395 - PS417_27020 0.65 -1.6 5,835,448 + PS417_27020 0.71 -0.5 5,835,483 + PS417_27020 0.75 +0.7 5,835,483 + PS417_27020 0.75 -0.0 5,835,483 + PS417_27020 0.75 +0.3 5,835,484 - PS417_27020 0.75 +0.7 5,835,522 - PS417_27020 0.79 -1.0 5,835,746 + -0.5 5,835,747 - +0.1 5,835,766 - +2.2 5,835,766 - +0.1 5,835,772 - +0.6 5,835,772 - -0.3 5,835,823 - -0.3 5,835,834 + -0.1 5,835,882 + -1.1 5,835,882 + -0.7 5,835,883 - -0.4 5,835,883 - -0.8 5,835,883 - -2.0 5,836,150 + PS417_27025 0.34 -0.1 5,836,257 + PS417_27025 0.46 -2.1 5,836,257 + PS417_27025 0.46 -0.1 5,836,257 + PS417_27025 0.46 -1.4 5,836,258 - PS417_27025 0.46 -0.2 5,836,258 - PS417_27025 0.46 -0.6 5,836,258 - PS417_27025 0.46 -1.2 5,836,494 + PS417_27025 0.71 -2.0 5,836,494 + PS417_27025 0.71 +0.9 5,836,495 - PS417_27025 0.71 -0.3 5,836,561 - PS417_27025 0.79 -0.1 5,836,561 - PS417_27025 0.79 -1.6 5,836,575 - PS417_27025 0.80 -0.9 5,836,787 + +0.8 5,836,788 - -0.3 5,836,890 - -0.7 5,837,594 - PS417_27030 0.89 -1.2 5,837,649 + -1.0 5,837,649 + +0.1 5,837,649 + -0.3 5,837,649 + -0.1 5,837,649 + +0.8 5,837,649 + -1.8 5,837,649 + -0.4 5,837,650 - +0.6 5,837,650 - -0.3 5,837,662 - -0.3
Or see this region's nucleotide sequence