Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26760 and PS417_26765 are separated by 19 nucleotides PS417_26765 and PS417_26770 are separated by 144 nucleotides PS417_26770 and PS417_26775 are separated by 9 nucleotides PS417_26775 and PS417_26780 are separated by 52 nucleotides
PS417_26760: PS417_26760 - oxidase, at 5,780,970 to 5,782,172
_26760
PS417_26765: PS417_26765 - membrane protein, at 5,782,192 to 5,782,515
_26765
PS417_26770: PS417_26770 - tRNA (guanine-N7)-methyltransferase, at 5,782,660 to 5,783,385
_26770
PS417_26775: PS417_26775 - thiazole synthase, at 5,783,395 to 5,784,189
_26775
PS417_26780: PS417_26780 - thiamine biosynthesis protein ThiS, at 5,784,242 to 5,784,442
_26780
Position (kb)
5782
5783
5784 Strain fitness (log2 ratio)
-2
-1
0
1 at 5781.738 kb on + strand, within PS417_26760 at 5781.845 kb on + strand, within PS417_26760 at 5781.900 kb on + strand, within PS417_26760 at 5782.088 kb on - strand at 5782.088 kb on - strand at 5782.180 kb on + strand at 5782.405 kb on + strand, within PS417_26765 at 5782.446 kb on + strand, within PS417_26765 at 5782.446 kb on + strand, within PS417_26765 at 5782.446 kb on + strand, within PS417_26765 at 5782.450 kb on + strand, within PS417_26765 at 5782.565 kb on - strand at 5782.565 kb on - strand at 5782.820 kb on + strand, within PS417_26770 at 5783.027 kb on + strand, within PS417_26770 at 5783.028 kb on - strand, within PS417_26770 at 5783.247 kb on - strand, within PS417_26770 at 5783.397 kb on - strand at 5783.397 kb on - strand at 5783.397 kb on - strand at 5783.397 kb on - strand at 5783.466 kb on - strand at 5783.466 kb on - strand at 5783.771 kb on + strand, within PS417_26775 at 5783.771 kb on + strand at 5783.772 kb on - strand, within PS417_26775 at 5783.942 kb on + strand, within PS417_26775 at 5783.973 kb on - strand, within PS417_26775 at 5784.107 kb on + strand, within PS417_26775 at 5784.141 kb on - strand at 5784.144 kb on + strand at 5784.144 kb on + strand at 5784.174 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,781,738 + PS417_26760 0.64 +0.1 5,781,845 + PS417_26760 0.73 -1.8 5,781,900 + PS417_26760 0.77 -1.4 5,782,088 - +0.8 5,782,088 - -2.3 5,782,180 + +0.7 5,782,405 + PS417_26765 0.66 -0.6 5,782,446 + PS417_26765 0.78 -0.7 5,782,446 + PS417_26765 0.78 -0.5 5,782,446 + PS417_26765 0.78 +0.5 5,782,450 + PS417_26765 0.80 +0.2 5,782,565 - +0.4 5,782,565 - +0.4 5,782,820 + PS417_26770 0.22 -0.1 5,783,027 + PS417_26770 0.51 -0.1 5,783,028 - PS417_26770 0.51 -0.7 5,783,247 - PS417_26770 0.81 -1.6 5,783,397 - -0.1 5,783,397 - -0.7 5,783,397 - -0.1 5,783,397 - -0.4 5,783,466 - +0.8 5,783,466 - -0.1 5,783,771 + PS417_26775 0.47 -0.8 5,783,771 + -1.3 5,783,772 - PS417_26775 0.47 +0.5 5,783,942 + PS417_26775 0.69 -0.7 5,783,973 - PS417_26775 0.73 -0.9 5,784,107 + PS417_26775 0.90 +1.5 5,784,141 - -1.8 5,784,144 + -0.8 5,784,144 + +0.4 5,784,174 + +1.2
Or see this region's nucleotide sequence