Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23605 and PS417_23610 are separated by 194 nucleotides PS417_23610 and PS417_23615 are separated by 122 nucleotides PS417_23615 and PS417_23620 are separated by 196 nucleotides
PS417_23605: PS417_23605 - permease DsdX, at 5,104,968 to 5,106,371
_23605
PS417_23610: PS417_23610 - hypothetical protein, at 5,106,566 to 5,106,946
_23610
PS417_23615: PS417_23615 - sulfate transporter, at 5,107,069 to 5,108,514
_23615
PS417_23620: PS417_23620 - epimerase, at 5,108,711 to 5,109,604
_23620
Position (kb)
5107
5108
5109 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5106.283 kb on + strand at 5106.284 kb on - strand at 5106.284 kb on - strand at 5106.480 kb on + strand at 5106.480 kb on + strand at 5106.480 kb on + strand at 5106.480 kb on + strand at 5106.480 kb on + strand at 5106.481 kb on - strand at 5106.481 kb on - strand at 5106.481 kb on - strand at 5106.530 kb on - strand at 5106.530 kb on - strand at 5106.595 kb on + strand at 5106.635 kb on - strand, within PS417_23610 at 5106.740 kb on + strand, within PS417_23610 at 5106.740 kb on + strand, within PS417_23610 at 5106.740 kb on + strand at 5106.811 kb on - strand, within PS417_23610 at 5106.815 kb on + strand at 5106.815 kb on + strand, within PS417_23610 at 5106.962 kb on + strand at 5107.056 kb on + strand at 5107.847 kb on + strand, within PS417_23615 at 5107.968 kb on - strand, within PS417_23615 at 5108.273 kb on + strand, within PS417_23615 at 5108.274 kb on - strand, within PS417_23615 at 5108.308 kb on + strand, within PS417_23615 at 5108.520 kb on - strand at 5108.540 kb on - strand at 5108.540 kb on - strand at 5108.577 kb on + strand at 5108.580 kb on + strand at 5108.580 kb on + strand at 5108.580 kb on + strand at 5108.581 kb on - strand at 5108.581 kb on - strand at 5108.581 kb on - strand at 5108.581 kb on - strand at 5108.587 kb on + strand at 5108.588 kb on - strand at 5108.702 kb on - strand at 5108.749 kb on + strand at 5108.750 kb on - strand at 5108.750 kb on - strand at 5108.820 kb on + strand, within PS417_23620 at 5108.821 kb on - strand, within PS417_23620 at 5108.821 kb on - strand, within PS417_23620 at 5108.821 kb on - strand, within PS417_23620 at 5108.821 kb on - strand, within PS417_23620 at 5108.991 kb on + strand, within PS417_23620 at 5108.992 kb on - strand, within PS417_23620 at 5109.258 kb on + strand, within PS417_23620 at 5109.259 kb on - strand, within PS417_23620 at 5109.259 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,106,283 + +0.9 5,106,284 - -0.1 5,106,284 - -1.1 5,106,480 + -0.4 5,106,480 + +0.2 5,106,480 + -0.6 5,106,480 + +0.2 5,106,480 + +0.0 5,106,481 - -1.9 5,106,481 - +1.8 5,106,481 - +1.1 5,106,530 - +0.1 5,106,530 - -0.2 5,106,595 + +0.6 5,106,635 - PS417_23610 0.18 -0.2 5,106,740 + PS417_23610 0.46 -0.5 5,106,740 + PS417_23610 0.46 +1.4 5,106,740 + -0.3 5,106,811 - PS417_23610 0.64 -0.6 5,106,815 + +1.5 5,106,815 + PS417_23610 0.65 +0.8 5,106,962 + +0.5 5,107,056 + +0.2 5,107,847 + PS417_23615 0.54 +0.4 5,107,968 - PS417_23615 0.62 -0.6 5,108,273 + PS417_23615 0.83 -1.1 5,108,274 - PS417_23615 0.83 -0.8 5,108,308 + PS417_23615 0.86 -1.3 5,108,520 - -0.4 5,108,540 - +0.1 5,108,540 - +0.6 5,108,577 + -0.5 5,108,580 + -1.2 5,108,580 + -0.4 5,108,580 + +1.5 5,108,581 - +0.9 5,108,581 - -0.0 5,108,581 - -0.4 5,108,581 - -0.2 5,108,587 + -0.7 5,108,588 - +0.0 5,108,702 - -0.1 5,108,749 + +0.2 5,108,750 - -0.1 5,108,750 - -0.4 5,108,820 + PS417_23620 0.12 +0.5 5,108,821 - PS417_23620 0.12 +0.4 5,108,821 - PS417_23620 0.12 -1.6 5,108,821 - PS417_23620 0.12 -0.0 5,108,821 - PS417_23620 0.12 +1.2 5,108,991 + PS417_23620 0.31 +0.3 5,108,992 - PS417_23620 0.31 -1.4 5,109,258 + PS417_23620 0.61 +0.0 5,109,259 - PS417_23620 0.61 -0.5 5,109,259 - +0.5
Or see this region's nucleotide sequence