Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23480 and PS417_23485 are separated by 216 nucleotides PS417_23485 and PS417_23490 are separated by 57 nucleotides PS417_23490 and PS417_23495 are separated by 20 nucleotides PS417_23495 and PS417_23500 are separated by 2 nucleotides PS417_23500 and PS417_23505 are separated by 23 nucleotides
PS417_23480: PS417_23480 - MerR family transcriptional regulator, at 5,083,348 to 5,084,298
_23480
PS417_23485: PS417_23485 - antibiotic biosynthesis monooxygenase, at 5,084,515 to 5,085,072
_23485
PS417_23490: PS417_23490 - dihydrofolate reductase, at 5,085,130 to 5,085,840
_23490
PS417_23495: PS417_23495 - GTP cyclohydrolase, at 5,085,861 to 5,086,415
_23495
PS417_23500: PS417_23500 - D-erythro-7,8-dihydroneopterin triphosphate epimerase, at 5,086,418 to 5,086,789
_23500
PS417_23505: PS417_23505 - cell division protein DedD, at 5,086,813 to 5,087,109
_23505
Position (kb) 5085
5086 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5084.286 kb on + strand at 5084.286 kb on + strand at 5084.287 kb on - strand at 5084.397 kb on - strand at 5084.415 kb on + strand at 5084.416 kb on - strand at 5084.476 kb on - strand at 5084.480 kb on - strand at 5084.509 kb on + strand at 5084.510 kb on - strand at 5084.520 kb on + strand at 5084.520 kb on + strand at 5084.521 kb on - strand at 5084.521 kb on - strand at 5084.796 kb on - strand, within PS417_23485 at 5084.796 kb on - strand, within PS417_23485 at 5084.796 kb on - strand at 5085.075 kb on - strand at 5085.096 kb on + strand at 5085.098 kb on + strand at 5085.099 kb on - strand at 5085.351 kb on + strand, within PS417_23490 at 5085.572 kb on - strand, within PS417_23490 at 5085.790 kb on + strand at 5085.790 kb on + strand at 5085.790 kb on + strand at 5085.790 kb on + strand at 5085.791 kb on - strand at 5085.831 kb on + strand at 5085.838 kb on + strand at 5085.838 kb on + strand at 5085.839 kb on - strand at 5085.860 kb on + strand at 5085.860 kb on + strand at 5085.860 kb on + strand at 5085.860 kb on + strand at 5085.860 kb on + strand at 5085.861 kb on - strand at 5085.861 kb on - strand at 5085.861 kb on - strand at 5086.068 kb on + strand, within PS417_23495 at 5086.069 kb on - strand, within PS417_23495 at 5086.122 kb on + strand, within PS417_23495 at 5086.122 kb on + strand, within PS417_23495 at 5086.123 kb on - strand, within PS417_23495 at 5086.123 kb on - strand, within PS417_23495 at 5086.123 kb on - strand, within PS417_23495 at 5086.179 kb on + strand, within PS417_23495 at 5086.179 kb on + strand, within PS417_23495 at 5086.179 kb on + strand, within PS417_23495 at 5086.180 kb on - strand, within PS417_23495 at 5086.180 kb on - strand, within PS417_23495 at 5086.180 kb on - strand, within PS417_23495 at 5086.180 kb on - strand, within PS417_23495 at 5086.413 kb on + strand at 5086.413 kb on + strand at 5086.414 kb on - strand at 5086.414 kb on - strand at 5086.414 kb on - strand at 5086.478 kb on + strand at 5086.478 kb on + strand, within PS417_23500 at 5086.478 kb on + strand, within PS417_23500 at 5086.478 kb on + strand, within PS417_23500 at 5086.479 kb on - strand, within PS417_23500 at 5086.479 kb on - strand, within PS417_23500 at 5086.479 kb on - strand, within PS417_23500 at 5086.596 kb on - strand, within PS417_23500
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,084,286 + +0.5 5,084,286 + -0.4 5,084,287 - -1.9 5,084,397 - -1.1 5,084,415 + +0.4 5,084,416 - +1.0 5,084,476 - -0.1 5,084,480 - -1.5 5,084,509 + +0.3 5,084,510 - +0.9 5,084,520 + +0.7 5,084,520 + +0.3 5,084,521 - +0.7 5,084,521 - -0.2 5,084,796 - PS417_23485 0.50 +0.3 5,084,796 - PS417_23485 0.50 -0.3 5,084,796 - +0.5 5,085,075 - +0.7 5,085,096 + +0.1 5,085,098 + -1.0 5,085,099 - -0.5 5,085,351 + PS417_23490 0.31 -2.5 5,085,572 - PS417_23490 0.62 -1.2 5,085,790 + -1.1 5,085,790 + +0.5 5,085,790 + -1.2 5,085,790 + -1.3 5,085,791 - -1.1 5,085,831 + -0.5 5,085,838 + +0.2 5,085,838 + +1.5 5,085,839 - +1.9 5,085,860 + -1.1 5,085,860 + -0.5 5,085,860 + -0.2 5,085,860 + +0.6 5,085,860 + +0.1 5,085,861 - +0.7 5,085,861 - +0.5 5,085,861 - +0.5 5,086,068 + PS417_23495 0.37 +0.5 5,086,069 - PS417_23495 0.37 +0.5 5,086,122 + PS417_23495 0.47 +0.1 5,086,122 + PS417_23495 0.47 +0.8 5,086,123 - PS417_23495 0.47 -0.2 5,086,123 - PS417_23495 0.47 +1.8 5,086,123 - PS417_23495 0.47 +0.5 5,086,179 + PS417_23495 0.57 +0.5 5,086,179 + PS417_23495 0.57 +0.1 5,086,179 + PS417_23495 0.57 -0.2 5,086,180 - PS417_23495 0.57 +0.1 5,086,180 - PS417_23495 0.57 +0.8 5,086,180 - PS417_23495 0.57 -0.1 5,086,180 - PS417_23495 0.57 +0.1 5,086,413 + -0.0 5,086,413 + +1.1 5,086,414 - +0.2 5,086,414 - -0.8 5,086,414 - +0.5 5,086,478 + +1.1 5,086,478 + PS417_23500 0.16 -0.7 5,086,478 + PS417_23500 0.16 -0.1 5,086,478 + PS417_23500 0.16 +0.6 5,086,479 - PS417_23500 0.16 -0.3 5,086,479 - PS417_23500 0.16 -0.0 5,086,479 - PS417_23500 0.16 -0.8 5,086,596 - PS417_23500 0.48 -0.4
Or see this region's nucleotide sequence