Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23470 and PS417_23475 are separated by 65 nucleotides PS417_23475 and PS417_23480 are separated by 2 nucleotides PS417_23480 and PS417_23485 are separated by 216 nucleotides PS417_23485 and PS417_23490 are separated by 57 nucleotides
PS417_23470: PS417_23470 - histidine kinase, at 5,082,207 to 5,082,674
_23470
PS417_23475: PS417_23475 - hypothetical protein, at 5,082,740 to 5,083,345
_23475
PS417_23480: PS417_23480 - MerR family transcriptional regulator, at 5,083,348 to 5,084,298
_23480
PS417_23485: PS417_23485 - antibiotic biosynthesis monooxygenase, at 5,084,515 to 5,085,072
_23485
PS417_23490: PS417_23490 - dihydrofolate reductase, at 5,085,130 to 5,085,840
_23490
Position (kb)
5083
5084
5085 Strain fitness (log2 ratio)
-2
-1
0
1 at 5082.513 kb on + strand, within PS417_23470 at 5082.513 kb on + strand, within PS417_23470 at 5082.513 kb on + strand, within PS417_23470 at 5082.514 kb on - strand, within PS417_23470 at 5082.537 kb on + strand, within PS417_23470 at 5082.537 kb on + strand, within PS417_23470 at 5082.537 kb on + strand, within PS417_23470 at 5082.537 kb on + strand, within PS417_23470 at 5083.099 kb on - strand, within PS417_23475 at 5084.286 kb on + strand at 5084.286 kb on + strand at 5084.287 kb on - strand at 5084.397 kb on - strand at 5084.415 kb on + strand at 5084.416 kb on - strand at 5084.476 kb on - strand at 5084.480 kb on - strand at 5084.509 kb on + strand at 5084.510 kb on - strand at 5084.520 kb on + strand at 5084.520 kb on + strand at 5084.521 kb on - strand at 5084.521 kb on - strand at 5084.796 kb on - strand, within PS417_23485 at 5084.796 kb on - strand, within PS417_23485 at 5084.796 kb on - strand at 5085.075 kb on - strand at 5085.096 kb on + strand at 5085.098 kb on + strand at 5085.099 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,082,513 + PS417_23470 0.65 -0.4 5,082,513 + PS417_23470 0.65 +1.2 5,082,513 + PS417_23470 0.65 +0.6 5,082,514 - PS417_23470 0.66 +0.4 5,082,537 + PS417_23470 0.71 +0.4 5,082,537 + PS417_23470 0.71 -0.2 5,082,537 + PS417_23470 0.71 +0.5 5,082,537 + PS417_23470 0.71 -0.4 5,083,099 - PS417_23475 0.59 +0.3 5,084,286 + +0.5 5,084,286 + -0.4 5,084,287 - -1.9 5,084,397 - -1.1 5,084,415 + +0.4 5,084,416 - +1.0 5,084,476 - -0.1 5,084,480 - -1.5 5,084,509 + +0.3 5,084,510 - +0.9 5,084,520 + +0.7 5,084,520 + +0.3 5,084,521 - +0.7 5,084,521 - -0.2 5,084,796 - PS417_23485 0.50 +0.3 5,084,796 - PS417_23485 0.50 -0.3 5,084,796 - +0.5 5,085,075 - +0.7 5,085,096 + +0.1 5,085,098 + -1.0 5,085,099 - -0.5
Or see this region's nucleotide sequence