Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02030 and PS417_02035 overlap by 1 nucleotides PS417_02035 and PS417_02040 overlap by 4 nucleotides PS417_02040 and PS417_02045 overlap by 4 nucleotides
PS417_02030: PS417_02030 - 3-oxoacyl-ACP synthase, at 456,400 to 457,626
_02030
PS417_02035: PS417_02035 - 3-ketoacyl-ACP reductase, at 457,626 to 458,354
_02035
PS417_02040: PS417_02040 - 3-hydroxylacyl-ACP dehydratase, at 458,351 to 458,812
_02040
PS417_02045: PS417_02045 - 3-oxoacyl-ACP synthase, at 458,809 to 459,975
_02045
Position (kb) 457
458
459 Strain fitness (log2 ratio) -2
-1
0
1 at 456.733 kb on + strand, within PS417_02030 at 456.734 kb on - strand, within PS417_02030 at 457.011 kb on - strand at 457.224 kb on - strand, within PS417_02030 at 457.224 kb on - strand, within PS417_02030 at 457.224 kb on - strand, within PS417_02030 at 457.669 kb on + strand at 457.670 kb on - strand at 457.897 kb on + strand, within PS417_02035 at 457.898 kb on - strand, within PS417_02035 at 457.953 kb on - strand, within PS417_02035 at 458.009 kb on - strand, within PS417_02035 at 458.114 kb on - strand, within PS417_02035 at 458.378 kb on + strand at 458.379 kb on - strand at 458.408 kb on + strand, within PS417_02040 at 458.408 kb on + strand, within PS417_02040 at 458.409 kb on - strand, within PS417_02040 at 458.409 kb on - strand, within PS417_02040 at 458.914 kb on + strand at 458.914 kb on + strand at 458.915 kb on - strand at 459.234 kb on + strand, within PS417_02045 at 459.235 kb on - strand, within PS417_02045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 456,733 + PS417_02030 0.27 +0.2 456,734 - PS417_02030 0.27 -0.2 457,011 - -0.6 457,224 - PS417_02030 0.67 -0.1 457,224 - PS417_02030 0.67 -0.5 457,224 - PS417_02030 0.67 +1.6 457,669 + +1.5 457,670 - -0.6 457,897 + PS417_02035 0.37 -1.8 457,898 - PS417_02035 0.37 -1.1 457,953 - PS417_02035 0.45 -1.4 458,009 - PS417_02035 0.53 -2.7 458,114 - PS417_02035 0.67 -0.0 458,378 + +0.3 458,379 - +0.5 458,408 + PS417_02040 0.12 -0.2 458,408 + PS417_02040 0.12 +0.1 458,409 - PS417_02040 0.13 -0.4 458,409 - PS417_02040 0.13 -0.5 458,914 + +0.1 458,914 + +0.7 458,915 - +0.4 459,234 + PS417_02045 0.36 -0.6 459,235 - PS417_02045 0.37 -0.3
Or see this region's nucleotide sequence