Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17395 and PS417_17400 are separated by 116 nucleotides PS417_17400 and PS417_17405 are separated by 177 nucleotides PS417_17405 and PS417_17410 are separated by 184 nucleotides
PS417_17395: PS417_17395 - MFS transporter, at 3,799,291 to 3,800,934
_17395
PS417_17400: PS417_17400 - TetR family transcriptional regulator, at 3,801,051 to 3,801,638
_17400
PS417_17405: PS417_17405 - AraC family transcriptional regulator, at 3,801,816 to 3,802,604
_17405
PS417_17410: PS417_17410 - membrane protein, at 3,802,789 to 3,804,267
_17410
Position (kb)
3801
3802
3803 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3800.834 kb on + strand at 3800.924 kb on + strand at 3800.924 kb on + strand at 3800.924 kb on + strand at 3800.924 kb on + strand at 3800.925 kb on - strand at 3800.925 kb on - strand at 3800.925 kb on - strand at 3800.925 kb on - strand at 3800.929 kb on - strand at 3800.944 kb on + strand at 3800.944 kb on + strand at 3800.978 kb on + strand at 3800.987 kb on + strand at 3800.987 kb on + strand at 3801.241 kb on + strand, within PS417_17400 at 3801.242 kb on - strand, within PS417_17400 at 3801.394 kb on - strand, within PS417_17400 at 3801.496 kb on + strand, within PS417_17400 at 3801.496 kb on + strand, within PS417_17400 at 3801.496 kb on + strand at 3801.497 kb on - strand, within PS417_17400 at 3801.557 kb on - strand, within PS417_17400 at 3801.811 kb on + strand at 3801.811 kb on + strand at 3801.811 kb on + strand at 3801.816 kb on - strand at 3801.816 kb on - strand at 3801.835 kb on + strand at 3801.836 kb on - strand at 3801.873 kb on + strand at 3802.220 kb on + strand, within PS417_17405 at 3802.272 kb on + strand, within PS417_17405 at 3802.303 kb on + strand, within PS417_17405 at 3802.605 kb on + strand at 3802.726 kb on + strand at 3802.948 kb on - strand, within PS417_17410 at 3803.325 kb on - strand, within PS417_17410 at 3803.440 kb on + strand, within PS417_17410
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,800,834 + +0.4 3,800,924 + +0.6 3,800,924 + -1.1 3,800,924 + +3.0 3,800,924 + +0.1 3,800,925 - -1.3 3,800,925 - -0.4 3,800,925 - +0.3 3,800,925 - -0.1 3,800,929 - -0.5 3,800,944 + -0.0 3,800,944 + +0.2 3,800,978 + -0.3 3,800,987 + +0.7 3,800,987 + -0.7 3,801,241 + PS417_17400 0.32 +1.0 3,801,242 - PS417_17400 0.32 +0.5 3,801,394 - PS417_17400 0.58 -0.6 3,801,496 + PS417_17400 0.76 -1.0 3,801,496 + PS417_17400 0.76 -1.5 3,801,496 + -1.3 3,801,497 - PS417_17400 0.76 -0.1 3,801,557 - PS417_17400 0.86 -1.0 3,801,811 + -1.8 3,801,811 + -0.2 3,801,811 + +0.9 3,801,816 - +0.2 3,801,816 - +0.1 3,801,835 + -0.1 3,801,836 - +0.4 3,801,873 + +0.2 3,802,220 + PS417_17405 0.51 -0.3 3,802,272 + PS417_17405 0.58 +1.4 3,802,303 + PS417_17405 0.62 +0.3 3,802,605 + -0.5 3,802,726 + +0.7 3,802,948 - PS417_17410 0.11 -0.4 3,803,325 - PS417_17410 0.36 +0.1 3,803,440 + PS417_17410 0.44 +0.0
Or see this region's nucleotide sequence