Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16130 and PS417_16135 are separated by 145 nucleotides PS417_16135 and PS417_16140 are separated by 155 nucleotides PS417_16140 and PS417_16145 overlap by 7 nucleotides
PS417_16130: PS417_16130 - quercetin 2,3-dioxygenase, at 3,538,007 to 3,538,729
_16130
PS417_16135: PS417_16135 - hypothetical protein, at 3,538,875 to 3,539,261
_16135
PS417_16140: PS417_16140 - ABC transporter, at 3,539,417 to 3,540,205
_16140
PS417_16145: PS417_16145 - sulfonate ABC transporter permease, at 3,540,199 to 3,541,188
_16145
Position (kb)
3538
3539
3540 Strain fitness (log2 ratio)
-2
-1
0
1 at 3538.205 kb on + strand, within PS417_16130 at 3538.205 kb on + strand, within PS417_16130 at 3538.205 kb on + strand, within PS417_16130 at 3538.206 kb on - strand, within PS417_16130 at 3538.565 kb on + strand, within PS417_16130 at 3538.565 kb on + strand, within PS417_16130 at 3538.565 kb on + strand, within PS417_16130 at 3538.565 kb on + strand, within PS417_16130 at 3538.566 kb on - strand, within PS417_16130 at 3538.566 kb on - strand, within PS417_16130 at 3538.852 kb on + strand at 3539.061 kb on - strand, within PS417_16135 at 3539.325 kb on - strand at 3539.391 kb on + strand at 3539.391 kb on + strand at 3539.391 kb on + strand at 3539.392 kb on - strand at 3539.399 kb on - strand at 3539.475 kb on + strand at 3539.663 kb on - strand, within PS417_16140 at 3540.153 kb on - strand at 3540.182 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,538,205 + PS417_16130 0.27 +0.4 3,538,205 + PS417_16130 0.27 -0.5 3,538,205 + PS417_16130 0.27 -0.3 3,538,206 - PS417_16130 0.28 -0.2 3,538,565 + PS417_16130 0.77 -0.3 3,538,565 + PS417_16130 0.77 +0.1 3,538,565 + PS417_16130 0.77 -2.0 3,538,565 + PS417_16130 0.77 -0.1 3,538,566 - PS417_16130 0.77 -0.3 3,538,566 - PS417_16130 0.77 -1.5 3,538,852 + +0.1 3,539,061 - PS417_16135 0.48 -1.3 3,539,325 - +0.4 3,539,391 + +0.5 3,539,391 + +0.1 3,539,391 + -0.3 3,539,392 - -0.0 3,539,399 - -1.1 3,539,475 + -0.1 3,539,663 - PS417_16140 0.31 -1.6 3,540,153 - -0.4 3,540,182 + +0.1
Or see this region's nucleotide sequence