Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15660 and PS417_15665 are separated by 65 nucleotides PS417_15665 and PS417_15670 overlap by 12 nucleotides PS417_15670 and PS417_15675 are separated by 163 nucleotides PS417_15675 and PS417_15680 are separated by 70 nucleotides PS417_15680 and PS417_15685 are separated by 23 nucleotides PS417_15685 and PS417_15690 are separated by 63 nucleotides
PS417_15660: PS417_15660 - hemerythrin, at 3,433,353 to 3,433,829
_15660
PS417_15665: PS417_15665 - hypothetical protein, at 3,433,895 to 3,434,173
_15665
PS417_15670: PS417_15670 - hypothetical protein, at 3,434,162 to 3,434,371
_15670
PS417_15675: PS417_15675 - chemotaxis protein, at 3,434,535 to 3,436,154
_15675
PS417_15680: PS417_15680 - radical SAM protein, at 3,436,225 to 3,436,893
_15680
PS417_15685: PS417_15685 - oxidoreductase, at 3,436,917 to 3,437,072
_15685
PS417_15690: PS417_15690 - ribonucleoside-triphosphate reductase, at 3,437,136 to 3,439,124
_15690
Position (kb)
3434
3435
3436
3437 Strain fitness (log2 ratio)
-1
0
1 at 3433.560 kb on + strand, within PS417_15660 at 3433.560 kb on + strand, within PS417_15660 at 3433.560 kb on + strand, within PS417_15660 at 3433.560 kb on + strand, within PS417_15660 at 3433.561 kb on - strand, within PS417_15660 at 3433.561 kb on - strand, within PS417_15660 at 3433.561 kb on - strand at 3433.725 kb on - strand, within PS417_15660 at 3433.856 kb on - strand at 3434.077 kb on + strand, within PS417_15665 at 3434.132 kb on - strand, within PS417_15665 at 3434.152 kb on + strand at 3434.153 kb on - strand at 3434.226 kb on + strand, within PS417_15670 at 3434.227 kb on - strand, within PS417_15670 at 3434.406 kb on + strand at 3434.744 kb on + strand, within PS417_15675 at 3435.616 kb on + strand, within PS417_15675 at 3435.616 kb on + strand, within PS417_15675 at 3435.796 kb on + strand, within PS417_15675 at 3435.796 kb on + strand, within PS417_15675 at 3435.796 kb on + strand, within PS417_15675 at 3436.075 kb on + strand at 3436.075 kb on + strand at 3437.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,433,560 + PS417_15660 0.43 +0.1 3,433,560 + PS417_15660 0.43 -1.6 3,433,560 + PS417_15660 0.43 -1.1 3,433,560 + PS417_15660 0.43 -1.5 3,433,561 - PS417_15660 0.44 +0.7 3,433,561 - PS417_15660 0.44 -0.2 3,433,561 - +0.1 3,433,725 - PS417_15660 0.78 +0.1 3,433,856 - -0.7 3,434,077 + PS417_15665 0.65 +0.1 3,434,132 - PS417_15665 0.85 +0.9 3,434,152 + +1.0 3,434,153 - -1.2 3,434,226 + PS417_15670 0.30 +0.8 3,434,227 - PS417_15670 0.31 -0.1 3,434,406 + -0.3 3,434,744 + PS417_15675 0.13 +0.5 3,435,616 + PS417_15675 0.67 -0.2 3,435,616 + PS417_15675 0.67 -0.7 3,435,796 + PS417_15675 0.78 -1.7 3,435,796 + PS417_15675 0.78 -1.7 3,435,796 + PS417_15675 0.78 -0.9 3,436,075 + -0.4 3,436,075 + +0.5 3,437,114 - -0.1
Or see this region's nucleotide sequence