Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14235 and PS417_14240 are separated by 254 nucleotides PS417_14240 and PS417_14245 are separated by 40 nucleotides PS417_14245 and PS417_14250 overlap by 1 nucleotides PS417_14250 and PS417_14255 are separated by 27 nucleotides
PS417_14235: PS417_14235 - hypothetical protein, at 3,116,992 to 3,117,189
_14235
PS417_14240: PS417_14240 - isochorismatase, at 3,117,444 to 3,118,049
_14240
PS417_14245: PS417_14245 - hypothetical protein, at 3,118,090 to 3,118,680
_14245
PS417_14250: PS417_14250 - hypothetical protein, at 3,118,680 to 3,119,378
_14250
PS417_14255: PS417_14255 - phosphate import ATP-binding protein PstB, at 3,119,406 to 3,120,167
_14255
Position (kb)
3118
3119 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3117.126 kb on + strand, within PS417_14235 at 3117.143 kb on - strand, within PS417_14235 at 3117.207 kb on - strand at 3117.207 kb on - strand at 3117.227 kb on + strand at 3117.228 kb on - strand at 3117.243 kb on + strand at 3117.243 kb on + strand at 3117.244 kb on - strand at 3117.244 kb on - strand at 3117.268 kb on - strand at 3117.341 kb on + strand at 3117.344 kb on - strand at 3117.353 kb on + strand at 3117.393 kb on + strand at 3117.393 kb on + strand at 3117.394 kb on - strand at 3117.394 kb on - strand at 3117.913 kb on - strand, within PS417_14240 at 3117.913 kb on - strand, within PS417_14240 at 3117.913 kb on - strand, within PS417_14240 at 3117.914 kb on + strand, within PS417_14240 at 3117.914 kb on + strand, within PS417_14240 at 3117.938 kb on + strand at 3118.047 kb on + strand at 3118.427 kb on + strand, within PS417_14245 at 3118.427 kb on + strand, within PS417_14245 at 3118.427 kb on + strand, within PS417_14245 at 3118.428 kb on - strand, within PS417_14245 at 3118.748 kb on + strand at 3118.748 kb on + strand at 3118.749 kb on - strand at 3118.795 kb on + strand, within PS417_14250 at 3118.912 kb on + strand, within PS417_14250 at 3118.980 kb on + strand, within PS417_14250 at 3118.981 kb on - strand, within PS417_14250 at 3118.981 kb on - strand, within PS417_14250 at 3118.985 kb on - strand, within PS417_14250 at 3118.985 kb on - strand, within PS417_14250 at 3118.985 kb on - strand, within PS417_14250 at 3119.032 kb on + strand, within PS417_14250 at 3119.041 kb on + strand, within PS417_14250 at 3119.042 kb on - strand, within PS417_14250 at 3119.110 kb on + strand, within PS417_14250 at 3119.146 kb on - strand, within PS417_14250 at 3119.215 kb on + strand, within PS417_14250 at 3119.215 kb on + strand, within PS417_14250 at 3119.216 kb on - strand, within PS417_14250 at 3119.216 kb on - strand, within PS417_14250 at 3119.216 kb on - strand, within PS417_14250 at 3119.429 kb on - strand at 3119.596 kb on + strand, within PS417_14255 at 3119.597 kb on - strand, within PS417_14255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,117,126 + PS417_14235 0.68 -0.3 3,117,143 - PS417_14235 0.76 +0.1 3,117,207 - -0.6 3,117,207 - -1.3 3,117,227 + -0.5 3,117,228 - +0.9 3,117,243 + -0.5 3,117,243 + +2.0 3,117,244 - +0.6 3,117,244 - -1.5 3,117,268 - -0.2 3,117,341 + +0.2 3,117,344 - +0.4 3,117,353 + -0.7 3,117,393 + +0.7 3,117,393 + +0.1 3,117,394 - -0.3 3,117,394 - +0.2 3,117,913 - PS417_14240 0.77 +0.1 3,117,913 - PS417_14240 0.77 -0.2 3,117,913 - PS417_14240 0.77 +1.4 3,117,914 + PS417_14240 0.78 -0.1 3,117,914 + PS417_14240 0.78 -0.9 3,117,938 + -1.1 3,118,047 + -0.3 3,118,427 + PS417_14245 0.57 -1.2 3,118,427 + PS417_14245 0.57 -2.0 3,118,427 + PS417_14245 0.57 -0.3 3,118,428 - PS417_14245 0.57 -0.0 3,118,748 + +1.4 3,118,748 + +1.4 3,118,749 - +0.2 3,118,795 + PS417_14250 0.16 +2.7 3,118,912 + PS417_14250 0.33 -0.4 3,118,980 + PS417_14250 0.43 +0.1 3,118,981 - PS417_14250 0.43 -1.3 3,118,981 - PS417_14250 0.43 -0.5 3,118,985 - PS417_14250 0.44 -2.1 3,118,985 - PS417_14250 0.44 -0.2 3,118,985 - PS417_14250 0.44 -0.4 3,119,032 + PS417_14250 0.50 +2.7 3,119,041 + PS417_14250 0.52 -1.8 3,119,042 - PS417_14250 0.52 -0.4 3,119,110 + PS417_14250 0.62 -0.6 3,119,146 - PS417_14250 0.67 -0.3 3,119,215 + PS417_14250 0.77 +0.2 3,119,215 + PS417_14250 0.77 +0.6 3,119,216 - PS417_14250 0.77 -0.1 3,119,216 - PS417_14250 0.77 +0.6 3,119,216 - PS417_14250 0.77 -0.6 3,119,429 - +0.1 3,119,596 + PS417_14255 0.25 +0.9 3,119,597 - PS417_14255 0.25 +1.2
Or see this region's nucleotide sequence