Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13585 and PS417_13590 are separated by 200 nucleotides PS417_13590 and PS417_13595 overlap by 4 nucleotides PS417_13595 and PS417_13600 are separated by 12 nucleotides
PS417_13585: PS417_13585 - alpha/beta hydrolase, at 2,992,126 to 2,992,926
_13585
PS417_13590: PS417_13590 - histidine kinase, at 2,993,127 to 2,994,491
_13590
PS417_13595: PS417_13595 - XRE family transcriptional regulator, at 2,994,488 to 2,995,150
_13595
PS417_13600: PS417_13600 - hypothetical protein, at 2,995,163 to 2,995,807
_13600
Position (kb)
2993
2994
2995 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2992.414 kb on + strand, within PS417_13585 at 2992.415 kb on - strand, within PS417_13585 at 2992.431 kb on + strand, within PS417_13585 at 2992.474 kb on + strand, within PS417_13585 at 2992.475 kb on - strand, within PS417_13585 at 2992.600 kb on + strand, within PS417_13585 at 2992.600 kb on + strand, within PS417_13585 at 2992.600 kb on + strand, within PS417_13585 at 2992.600 kb on + strand, within PS417_13585 at 2992.600 kb on + strand, within PS417_13585 at 2992.601 kb on - strand, within PS417_13585 at 2992.601 kb on - strand, within PS417_13585 at 2992.601 kb on - strand, within PS417_13585 at 2992.601 kb on - strand, within PS417_13585 at 2992.747 kb on + strand, within PS417_13585 at 2992.748 kb on - strand, within PS417_13585 at 2992.748 kb on - strand, within PS417_13585 at 2992.754 kb on + strand, within PS417_13585 at 2992.755 kb on - strand, within PS417_13585 at 2992.755 kb on - strand, within PS417_13585 at 2992.756 kb on + strand, within PS417_13585 at 2992.756 kb on + strand, within PS417_13585 at 2993.132 kb on + strand at 2993.133 kb on - strand at 2993.133 kb on - strand at 2993.133 kb on - strand at 2993.136 kb on + strand at 2993.136 kb on + strand at 2993.136 kb on + strand at 2993.263 kb on + strand at 2993.263 kb on + strand at 2993.332 kb on - strand, within PS417_13590 at 2993.724 kb on + strand, within PS417_13590 at 2993.995 kb on - strand, within PS417_13590 at 2993.999 kb on + strand, within PS417_13590 at 2994.179 kb on + strand, within PS417_13590 at 2994.511 kb on - strand at 2994.572 kb on - strand, within PS417_13595 at 2994.615 kb on + strand, within PS417_13595 at 2994.681 kb on - strand, within PS417_13595 at 2994.681 kb on - strand, within PS417_13595 at 2994.723 kb on + strand, within PS417_13595 at 2994.724 kb on - strand, within PS417_13595 at 2995.139 kb on + strand at 2995.139 kb on + strand at 2995.142 kb on + strand at 2995.142 kb on + strand at 2995.329 kb on + strand, within PS417_13600 at 2995.355 kb on - strand at 2995.434 kb on + strand, within PS417_13600 at 2995.434 kb on + strand, within PS417_13600 at 2995.455 kb on + strand, within PS417_13600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,992,414 + PS417_13585 0.36 -1.1 2,992,415 - PS417_13585 0.36 +0.2 2,992,431 + PS417_13585 0.38 +0.3 2,992,474 + PS417_13585 0.43 +0.5 2,992,475 - PS417_13585 0.44 -0.9 2,992,600 + PS417_13585 0.59 +0.3 2,992,600 + PS417_13585 0.59 +0.7 2,992,600 + PS417_13585 0.59 +1.3 2,992,600 + PS417_13585 0.59 +0.4 2,992,600 + PS417_13585 0.59 -0.1 2,992,601 - PS417_13585 0.59 -0.4 2,992,601 - PS417_13585 0.59 +0.2 2,992,601 - PS417_13585 0.59 -2.7 2,992,601 - PS417_13585 0.59 -0.9 2,992,747 + PS417_13585 0.78 -0.5 2,992,748 - PS417_13585 0.78 -0.9 2,992,748 - PS417_13585 0.78 -0.7 2,992,754 + PS417_13585 0.78 +1.0 2,992,755 - PS417_13585 0.79 -0.2 2,992,755 - PS417_13585 0.79 -0.1 2,992,756 + PS417_13585 0.79 +0.5 2,992,756 + PS417_13585 0.79 -0.2 2,993,132 + -0.0 2,993,133 - +0.1 2,993,133 - -0.2 2,993,133 - -0.1 2,993,136 + +1.9 2,993,136 + +0.1 2,993,136 + -0.4 2,993,263 + -1.2 2,993,263 + -0.2 2,993,332 - PS417_13590 0.15 +0.7 2,993,724 + PS417_13590 0.44 +0.1 2,993,995 - PS417_13590 0.64 -0.6 2,993,999 + PS417_13590 0.64 -0.7 2,994,179 + PS417_13590 0.77 +0.4 2,994,511 - -0.6 2,994,572 - PS417_13595 0.13 +0.4 2,994,615 + PS417_13595 0.19 +0.1 2,994,681 - PS417_13595 0.29 +1.6 2,994,681 - PS417_13595 0.29 -0.3 2,994,723 + PS417_13595 0.35 -0.8 2,994,724 - PS417_13595 0.36 -0.1 2,995,139 + +0.7 2,995,139 + -0.7 2,995,142 + -1.0 2,995,142 + +1.1 2,995,329 + PS417_13600 0.26 -2.0 2,995,355 - -0.3 2,995,434 + PS417_13600 0.42 -0.2 2,995,434 + PS417_13600 0.42 -0.3 2,995,455 + PS417_13600 0.45 -0.9
Or see this region's nucleotide sequence