Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13505 and PS417_13510 are separated by 118 nucleotides PS417_13510 and PS417_13515 are separated by 107 nucleotides
PS417_13505: PS417_13505 - alpha/beta hydrolase, at 2,977,391 to 2,978,323
_13505
PS417_13510: PS417_13510 - polyketide cyclase, at 2,978,442 to 2,978,876
_13510
PS417_13515: PS417_13515 - hypothetical protein, at 2,978,984 to 2,979,934
_13515
Position (kb)
2978
2979 Strain fitness (log2 ratio)
-2
-1
0
1 at 2977.624 kb on - strand, within PS417_13505 at 2977.835 kb on + strand, within PS417_13505 at 2977.835 kb on + strand, within PS417_13505 at 2977.835 kb on + strand, within PS417_13505 at 2977.836 kb on - strand, within PS417_13505 at 2977.836 kb on - strand, within PS417_13505 at 2977.836 kb on - strand, within PS417_13505 at 2978.027 kb on + strand, within PS417_13505 at 2978.130 kb on + strand, within PS417_13505 at 2978.238 kb on + strand at 2978.238 kb on + strand at 2978.700 kb on - strand, within PS417_13510 at 2978.893 kb on + strand at 2978.894 kb on - strand at 2978.923 kb on + strand at 2978.923 kb on + strand at 2978.924 kb on - strand at 2978.924 kb on - strand at 2978.924 kb on - strand at 2978.924 kb on - strand at 2978.924 kb on - strand at 2978.924 kb on - strand at 2978.966 kb on + strand at 2978.966 kb on + strand at 2978.967 kb on - strand at 2979.100 kb on - strand, within PS417_13515 at 2979.100 kb on - strand, within PS417_13515 at 2979.286 kb on - strand, within PS417_13515 at 2979.331 kb on - strand, within PS417_13515 at 2979.488 kb on - strand, within PS417_13515 at 2979.665 kb on + strand at 2979.676 kb on - strand, within PS417_13515 at 2979.721 kb on + strand, within PS417_13515 at 2979.721 kb on + strand, within PS417_13515 at 2979.722 kb on - strand, within PS417_13515 at 2979.722 kb on - strand, within PS417_13515 at 2979.846 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,977,624 - PS417_13505 0.25 +0.2 2,977,835 + PS417_13505 0.48 -1.6 2,977,835 + PS417_13505 0.48 -1.1 2,977,835 + PS417_13505 0.48 -0.9 2,977,836 - PS417_13505 0.48 -0.5 2,977,836 - PS417_13505 0.48 -0.1 2,977,836 - PS417_13505 0.48 -0.5 2,978,027 + PS417_13505 0.68 +0.2 2,978,130 + PS417_13505 0.79 -0.2 2,978,238 + -0.9 2,978,238 + +0.0 2,978,700 - PS417_13510 0.59 -1.6 2,978,893 + +0.0 2,978,894 - +0.7 2,978,923 + -0.1 2,978,923 + -0.9 2,978,924 - -0.7 2,978,924 - +1.1 2,978,924 - +0.0 2,978,924 - -0.6 2,978,924 - -0.4 2,978,924 - +1.0 2,978,966 + -0.6 2,978,966 + +0.1 2,978,967 - -0.2 2,979,100 - PS417_13515 0.12 -0.3 2,979,100 - PS417_13515 0.12 -0.2 2,979,286 - PS417_13515 0.32 +0.5 2,979,331 - PS417_13515 0.36 -0.2 2,979,488 - PS417_13515 0.53 -1.9 2,979,665 + +1.2 2,979,676 - PS417_13515 0.73 +0.2 2,979,721 + PS417_13515 0.77 -0.4 2,979,721 + PS417_13515 0.77 +0.0 2,979,722 - PS417_13515 0.78 -1.0 2,979,722 - PS417_13515 0.78 -0.3 2,979,846 + -0.2
Or see this region's nucleotide sequence