Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_13140 and PS417_13145 are separated by 32 nucleotides PS417_13145 and PS417_13150 are separated by 53 nucleotides PS417_13150 and PS417_13155 are separated by 125 nucleotides
PS417_13140: PS417_13140 - oxidoreductase, at 2,871,364 to 2,872,521
_13140
PS417_13145: PS417_13145 - 50S ribosomal protein L21, at 2,872,554 to 2,873,033
_13145
PS417_13150: PS417_13150 - TetR family transcriptional regulator, at 2,873,087 to 2,873,650
_13150
PS417_13155: PS417_13155 - phosphatidylserine synthase, at 2,873,776 to 2,875,119
_13155
Position (kb)
2872
2873
2874 Strain fitness (log2 ratio)
-1
0
1 at 2871.701 kb on - strand, within PS417_13140 at 2871.701 kb on - strand at 2871.961 kb on + strand, within PS417_13140 at 2872.150 kb on + strand, within PS417_13140 at 2872.463 kb on - strand at 2872.463 kb on - strand at 2872.463 kb on - strand at 2872.466 kb on + strand at 2872.466 kb on + strand at 2872.563 kb on + strand at 2872.563 kb on + strand at 2872.564 kb on - strand at 2872.750 kb on + strand, within PS417_13145 at 2872.757 kb on - strand, within PS417_13145 at 2872.811 kb on - strand, within PS417_13145 at 2873.013 kb on + strand at 2873.054 kb on - strand at 2873.736 kb on - strand at 2873.917 kb on + strand, within PS417_13155 at 2873.917 kb on + strand, within PS417_13155 at 2873.917 kb on + strand, within PS417_13155 at 2873.918 kb on - strand, within PS417_13155 at 2873.918 kb on - strand, within PS417_13155 at 2873.918 kb on - strand, within PS417_13155 at 2873.965 kb on + strand, within PS417_13155 at 2873.965 kb on + strand, within PS417_13155
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,871,701 - PS417_13140 0.29 -1.3 2,871,701 - -1.5 2,871,961 + PS417_13140 0.52 -1.5 2,872,150 + PS417_13140 0.68 -0.5 2,872,463 - +0.0 2,872,463 - +0.1 2,872,463 - +0.1 2,872,466 + +0.0 2,872,466 + -0.2 2,872,563 + +0.5 2,872,563 + -1.1 2,872,564 - -0.5 2,872,750 + PS417_13145 0.41 -0.7 2,872,757 - PS417_13145 0.42 -0.9 2,872,811 - PS417_13145 0.54 -1.2 2,873,013 + -0.4 2,873,054 - +0.1 2,873,736 - +0.1 2,873,917 + PS417_13155 0.10 -1.4 2,873,917 + PS417_13155 0.10 +0.2 2,873,917 + PS417_13155 0.10 -0.1 2,873,918 - PS417_13155 0.11 +0.0 2,873,918 - PS417_13155 0.11 +1.1 2,873,918 - PS417_13155 0.11 +0.0 2,873,965 + PS417_13155 0.14 +0.2 2,873,965 + PS417_13155 0.14 +0.8
Or see this region's nucleotide sequence