Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10155 and PS417_10160 are separated by 11 nucleotides PS417_10160 and PS417_10165 are separated by 2 nucleotides PS417_10165 and PS417_10170 are separated by 212 nucleotides PS417_10170 and PS417_10175 are separated by 82 nucleotides
PS417_10155: PS417_10155 - phage minor tail, at 2,174,587 to 2,174,925
_10155
PS417_10160: PS417_10160 - hypothetical protein, at 2,174,937 to 2,175,689
_10160
PS417_10165: PS417_10165 - hydrolase Nlp/P60, at 2,175,692 to 2,176,456
_10165
PS417_10170: PS417_10170 - hypothetical protein, at 2,176,669 to 2,177,286
_10170
PS417_10175: PS417_10175 - hypothetical protein, at 2,177,369 to 2,177,680
_10175
Position (kb)
2175
2176
2177 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2174.840 kb on - strand, within PS417_10155 at 2174.840 kb on - strand, within PS417_10155 at 2174.929 kb on - strand at 2175.113 kb on + strand, within PS417_10160 at 2175.114 kb on - strand, within PS417_10160 at 2175.114 kb on - strand, within PS417_10160 at 2175.114 kb on - strand, within PS417_10160 at 2175.117 kb on - strand, within PS417_10160 at 2175.135 kb on + strand, within PS417_10160 at 2175.135 kb on + strand at 2175.135 kb on + strand, within PS417_10160 at 2175.164 kb on + strand, within PS417_10160 at 2175.304 kb on - strand, within PS417_10160 at 2175.376 kb on - strand, within PS417_10160 at 2175.791 kb on + strand, within PS417_10165 at 2175.841 kb on - strand, within PS417_10165 at 2175.854 kb on + strand, within PS417_10165 at 2176.075 kb on + strand, within PS417_10165 at 2176.075 kb on + strand, within PS417_10165 at 2176.185 kb on - strand, within PS417_10165 at 2176.203 kb on + strand, within PS417_10165 at 2176.203 kb on + strand, within PS417_10165 at 2176.283 kb on + strand, within PS417_10165 at 2176.284 kb on - strand, within PS417_10165 at 2176.440 kb on + strand at 2176.440 kb on + strand at 2176.441 kb on - strand at 2176.470 kb on + strand at 2176.570 kb on + strand at 2176.608 kb on + strand at 2176.608 kb on + strand at 2176.608 kb on + strand at 2176.609 kb on - strand at 2176.609 kb on - strand at 2176.609 kb on - strand at 2176.614 kb on + strand at 2176.615 kb on - strand at 2176.623 kb on + strand at 2176.734 kb on + strand, within PS417_10170 at 2176.734 kb on + strand, within PS417_10170 at 2176.734 kb on + strand at 2176.988 kb on - strand, within PS417_10170 at 2177.132 kb on + strand, within PS417_10170 at 2177.133 kb on - strand, within PS417_10170 at 2177.170 kb on + strand at 2177.170 kb on + strand, within PS417_10170 at 2177.170 kb on + strand, within PS417_10170 at 2177.171 kb on - strand, within PS417_10170 at 2177.171 kb on - strand, within PS417_10170 at 2177.256 kb on + strand at 2177.256 kb on + strand at 2177.257 kb on - strand at 2177.303 kb on + strand at 2177.304 kb on - strand at 2177.367 kb on + strand at 2177.367 kb on + strand at 2177.370 kb on + strand at 2177.370 kb on + strand at 2177.370 kb on + strand at 2177.370 kb on + strand at 2177.370 kb on + strand at 2177.371 kb on - strand at 2177.371 kb on - strand at 2177.371 kb on - strand at 2177.371 kb on - strand at 2177.371 kb on - strand at 2177.371 kb on - strand at 2177.371 kb on - strand at 2177.386 kb on + strand at 2177.386 kb on + strand at 2177.386 kb on + strand at 2177.386 kb on + strand at 2177.387 kb on - strand at 2177.387 kb on - strand at 2177.447 kb on + strand, within PS417_10175 at 2177.447 kb on + strand, within PS417_10175 at 2177.448 kb on - strand, within PS417_10175 at 2177.448 kb on - strand, within PS417_10175 at 2177.452 kb on + strand, within PS417_10175 at 2177.453 kb on - strand, within PS417_10175
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,174,840 - PS417_10155 0.75 +0.4 2,174,840 - PS417_10155 0.75 -1.2 2,174,929 - -0.4 2,175,113 + PS417_10160 0.23 -0.9 2,175,114 - PS417_10160 0.24 -0.3 2,175,114 - PS417_10160 0.24 -0.6 2,175,114 - PS417_10160 0.24 +0.8 2,175,117 - PS417_10160 0.24 -1.3 2,175,135 + PS417_10160 0.26 -0.6 2,175,135 + -0.7 2,175,135 + PS417_10160 0.26 -0.9 2,175,164 + PS417_10160 0.30 -0.3 2,175,304 - PS417_10160 0.49 +0.1 2,175,376 - PS417_10160 0.58 +0.5 2,175,791 + PS417_10165 0.13 -1.6 2,175,841 - PS417_10165 0.19 -0.9 2,175,854 + PS417_10165 0.21 -1.5 2,176,075 + PS417_10165 0.50 +0.2 2,176,075 + PS417_10165 0.50 -0.2 2,176,185 - PS417_10165 0.64 -1.0 2,176,203 + PS417_10165 0.67 -1.3 2,176,203 + PS417_10165 0.67 +0.1 2,176,283 + PS417_10165 0.77 -0.1 2,176,284 - PS417_10165 0.77 -0.5 2,176,440 + -0.6 2,176,440 + -1.5 2,176,441 - -2.0 2,176,470 + -0.3 2,176,570 + -0.5 2,176,608 + -0.1 2,176,608 + +0.6 2,176,608 + -0.6 2,176,609 - -0.0 2,176,609 - -0.7 2,176,609 - +0.2 2,176,614 + +0.5 2,176,615 - -1.2 2,176,623 + +0.2 2,176,734 + PS417_10170 0.11 -0.6 2,176,734 + PS417_10170 0.11 +1.9 2,176,734 + +1.6 2,176,988 - PS417_10170 0.52 -0.1 2,177,132 + PS417_10170 0.75 -1.5 2,177,133 - PS417_10170 0.75 -0.8 2,177,170 + +1.6 2,177,170 + PS417_10170 0.81 +0.1 2,177,170 + PS417_10170 0.81 -0.0 2,177,171 - PS417_10170 0.81 +1.0 2,177,171 - PS417_10170 0.81 +0.1 2,177,256 + -1.3 2,177,256 + -0.6 2,177,257 - -0.5 2,177,303 + -1.7 2,177,304 - +2.0 2,177,367 + -0.3 2,177,367 + -0.3 2,177,370 + -1.1 2,177,370 + -0.2 2,177,370 + -0.9 2,177,370 + +1.9 2,177,370 + -1.9 2,177,371 - -0.4 2,177,371 - +0.0 2,177,371 - +0.0 2,177,371 - -0.0 2,177,371 - -0.7 2,177,371 - -1.1 2,177,371 - -1.6 2,177,386 + +0.5 2,177,386 + -0.2 2,177,386 + +0.6 2,177,386 + +3.4 2,177,387 - -0.4 2,177,387 - -0.9 2,177,447 + PS417_10175 0.25 -0.8 2,177,447 + PS417_10175 0.25 +0.6 2,177,448 - PS417_10175 0.25 +0.6 2,177,448 - PS417_10175 0.25 +0.3 2,177,452 + PS417_10175 0.27 -1.1 2,177,453 - PS417_10175 0.27 -0.2
Or see this region's nucleotide sequence