Strain Fitness in Pseudomonas simiae WCS417 around PS417_09975

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_09965 and PS417_09970 overlap by 4 nucleotidesPS417_09970 and PS417_09975 are separated by 270 nucleotidesPS417_09975 and PS417_09980 are separated by 76 nucleotidesPS417_09980 and PS417_09985 are separated by 4 nucleotides PS417_09965: PS417_09965 - hypothetical protein, at 2,147,942 to 2,148,856 _09965 PS417_09970: PS417_09970 - hypothetical protein, at 2,148,853 to 2,149,314 _09970 PS417_09975: PS417_09975 - hypothetical protein, at 2,149,585 to 2,149,893 _09975 PS417_09980: PS417_09980 - peptidase, at 2,149,970 to 2,150,197 _09980 PS417_09985: PS417_09985 - propanediol utilization protein, at 2,150,202 to 2,151,062 _09985 Position (kb) 2149 2150Strain fitness (log2 ratio) -2 -1 0 1at 2148.607 kb on + strand, within PS417_09965at 2148.608 kb on - strand, within PS417_09965at 2148.653 kb on + strandat 2148.654 kb on - strand, within PS417_09965at 2148.654 kb on - strand, within PS417_09965at 2148.731 kb on - strand, within PS417_09965at 2148.752 kb on + strand, within PS417_09965at 2148.753 kb on - strand, within PS417_09965at 2148.753 kb on - strand, within PS417_09965at 2148.799 kb on + strandat 2148.799 kb on + strandat 2148.856 kb on + strandat 2148.856 kb on + strandat 2148.856 kb on + strandat 2148.857 kb on - strandat 2148.857 kb on - strandat 2148.857 kb on - strandat 2148.859 kb on - strandat 2148.859 kb on - strandat 2148.907 kb on + strand, within PS417_09970at 2148.908 kb on - strand, within PS417_09970at 2148.908 kb on - strand, within PS417_09970at 2148.912 kb on + strand, within PS417_09970at 2148.912 kb on + strand, within PS417_09970at 2148.913 kb on - strand, within PS417_09970at 2148.923 kb on + strand, within PS417_09970at 2148.969 kb on - strand, within PS417_09970at 2148.976 kb on + strand, within PS417_09970at 2148.976 kb on + strandat 2148.977 kb on - strand, within PS417_09970at 2148.984 kb on - strand, within PS417_09970at 2149.069 kb on - strand, within PS417_09970at 2149.069 kb on - strand, within PS417_09970at 2149.198 kb on + strand, within PS417_09970at 2149.198 kb on + strand, within PS417_09970at 2149.199 kb on - strand, within PS417_09970at 2149.199 kb on - strand, within PS417_09970at 2149.204 kb on + strand, within PS417_09970at 2149.204 kb on + strand, within PS417_09970at 2149.248 kb on - strand, within PS417_09970at 2149.286 kb on - strandat 2149.340 kb on + strandat 2149.340 kb on + strandat 2149.340 kb on + strandat 2149.341 kb on - strandat 2149.341 kb on - strandat 2149.341 kb on - strandat 2149.341 kb on - strandat 2149.341 kb on - strandat 2149.342 kb on + strandat 2149.354 kb on + strandat 2149.501 kb on - strandat 2149.635 kb on + strand, within PS417_09975at 2149.655 kb on + strand, within PS417_09975at 2149.655 kb on + strand, within PS417_09975at 2149.656 kb on - strand, within PS417_09975at 2149.656 kb on - strand, within PS417_09975at 2149.743 kb on + strand, within PS417_09975at 2149.849 kb on - strand, within PS417_09975at 2149.849 kb on - strand, within PS417_09975at 2149.852 kb on + strand, within PS417_09975at 2149.935 kb on + strandat 2149.945 kb on + strandat 2149.958 kb on - strandat 2150.006 kb on + strand, within PS417_09980at 2150.047 kb on - strand, within PS417_09980at 2150.047 kb on - strand, within PS417_09980at 2150.054 kb on - strand, within PS417_09980at 2150.135 kb on + strand, within PS417_09980at 2150.169 kb on - strand, within PS417_09980at 2150.169 kb on - strand, within PS417_09980at 2150.169 kb on - strand, within PS417_09980

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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2,148,607 + PS417_09965 0.73 -0.7
2,148,608 - PS417_09965 0.73 -0.2
2,148,653 + -2.1
2,148,654 - PS417_09965 0.78 -0.2
2,148,654 - PS417_09965 0.78 +0.1
2,148,731 - PS417_09965 0.86 -0.7
2,148,752 + PS417_09965 0.89 +0.3
2,148,753 - PS417_09965 0.89 -0.7
2,148,753 - PS417_09965 0.89 -0.4
2,148,799 + -0.9
2,148,799 + -1.4
2,148,856 + -0.1
2,148,856 + +0.3
2,148,856 + +0.2
2,148,857 - +0.0
2,148,857 - -0.2
2,148,857 - -0.8
2,148,859 - -0.2
2,148,859 - -0.4
2,148,907 + PS417_09970 0.12 -0.3
2,148,908 - PS417_09970 0.12 -0.8
2,148,908 - PS417_09970 0.12 +0.1
2,148,912 + PS417_09970 0.13 +0.1
2,148,912 + PS417_09970 0.13 -0.1
2,148,913 - PS417_09970 0.13 -0.1
2,148,923 + PS417_09970 0.15 +0.4
2,148,969 - PS417_09970 0.25 +0.8
2,148,976 + PS417_09970 0.27 +0.1
2,148,976 + +1.6
2,148,977 - PS417_09970 0.27 -0.3
2,148,984 - PS417_09970 0.28 -1.6
2,149,069 - PS417_09970 0.47 +0.2
2,149,069 - PS417_09970 0.47 +0.4
2,149,198 + PS417_09970 0.75 +0.4
2,149,198 + PS417_09970 0.75 -0.9
2,149,199 - PS417_09970 0.75 -0.8
2,149,199 - PS417_09970 0.75 +1.2
2,149,204 + PS417_09970 0.76 -0.4
2,149,204 + PS417_09970 0.76 -0.2
2,149,248 - PS417_09970 0.85 +0.4
2,149,286 - -0.3
2,149,340 + -0.3
2,149,340 + +0.6
2,149,340 + -1.3
2,149,341 - -0.2
2,149,341 - -0.6
2,149,341 - -0.4
2,149,341 - -1.6
2,149,341 - +0.3
2,149,342 + -0.7
2,149,354 + -1.3
2,149,501 - -1.7
2,149,635 + PS417_09975 0.16 -2.3
2,149,655 + PS417_09975 0.23 -1.5
2,149,655 + PS417_09975 0.23 +0.0
2,149,656 - PS417_09975 0.23 +0.2
2,149,656 - PS417_09975 0.23 -1.6
2,149,743 + PS417_09975 0.51 -1.3
2,149,849 - PS417_09975 0.85 +0.6
2,149,849 - PS417_09975 0.85 -0.2
2,149,852 + PS417_09975 0.86 -1.3
2,149,935 + -0.6
2,149,945 + -1.6
2,149,958 - +0.8
2,150,006 + PS417_09980 0.16 +0.0
2,150,047 - PS417_09980 0.34 +0.3
2,150,047 - PS417_09980 0.34 +0.2
2,150,054 - PS417_09980 0.37 +0.0
2,150,135 + PS417_09980 0.72 -0.4
2,150,169 - PS417_09980 0.87 +1.4
2,150,169 - PS417_09980 0.87 -0.1
2,150,169 - PS417_09980 0.87 +1.4

Or see this region's nucleotide sequence