Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07810 and PS417_07815 are separated by 153 nucleotides PS417_07815 and PS417_07820 are separated by 261 nucleotides PS417_07820 and PS417_07825 overlap by 4 nucleotides PS417_07825 and PS417_07830 are separated by 61 nucleotides
PS417_07810: PS417_07810 - hypothetical protein, at 1,716,938 to 1,717,813
_07810
PS417_07815: PS417_07815 - hypothetical protein, at 1,717,967 to 1,718,191
_07815
PS417_07820: PS417_07820 - transposase, at 1,718,453 to 1,718,776
_07820
PS417_07825: PS417_07825 - transposase, at 1,718,773 to 1,719,108
_07825
PS417_07830: PS417_07830 - transposase, at 1,719,170 to 1,720,705
_07830
Position (kb)
1718
1719 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1717.477 kb on + strand, within PS417_07810 at 1717.477 kb on + strand, within PS417_07810 at 1717.498 kb on + strand, within PS417_07810 at 1717.617 kb on + strand, within PS417_07810 at 1717.618 kb on - strand, within PS417_07810 at 1717.618 kb on - strand, within PS417_07810 at 1717.618 kb on - strand, within PS417_07810 at 1717.763 kb on + strand at 1717.993 kb on + strand, within PS417_07815 at 1717.993 kb on + strand, within PS417_07815 at 1718.100 kb on + strand, within PS417_07815 at 1718.100 kb on + strand, within PS417_07815 at 1718.100 kb on + strand, within PS417_07815 at 1718.100 kb on + strand, within PS417_07815 at 1718.100 kb on + strand at 1718.145 kb on + strand, within PS417_07815 at 1718.146 kb on - strand, within PS417_07815 at 1718.169 kb on - strand at 1718.192 kb on - strand at 1718.204 kb on + strand at 1718.204 kb on + strand at 1718.204 kb on + strand at 1718.205 kb on - strand at 1718.205 kb on - strand at 1718.211 kb on - strand at 1718.273 kb on + strand at 1718.274 kb on - strand at 1718.274 kb on - strand at 1718.274 kb on - strand at 1718.274 kb on - strand at 1718.284 kb on + strand at 1718.284 kb on + strand at 1718.285 kb on - strand at 1718.285 kb on - strand at 1718.285 kb on - strand at 1718.293 kb on - strand at 1718.326 kb on + strand at 1718.326 kb on + strand at 1718.326 kb on + strand at 1718.326 kb on + strand at 1718.326 kb on + strand at 1718.326 kb on + strand at 1718.326 kb on + strand at 1718.326 kb on + strand at 1718.327 kb on - strand at 1718.327 kb on - strand at 1718.327 kb on - strand at 1718.327 kb on - strand at 1718.327 kb on - strand at 1718.327 kb on - strand at 1718.354 kb on + strand at 1718.437 kb on + strand at 1718.437 kb on + strand at 1718.438 kb on - strand at 1718.438 kb on - strand at 1718.438 kb on - strand at 1718.640 kb on + strand, within PS417_07820 at 1718.640 kb on + strand, within PS417_07820 at 1718.641 kb on - strand, within PS417_07820 at 1718.641 kb on - strand, within PS417_07820 at 1718.790 kb on + strand at 1718.790 kb on + strand at 1718.790 kb on + strand at 1718.791 kb on - strand at 1718.791 kb on - strand at 1718.890 kb on + strand, within PS417_07825 at 1718.890 kb on + strand, within PS417_07825 at 1718.890 kb on + strand, within PS417_07825 at 1718.891 kb on - strand, within PS417_07825 at 1718.891 kb on - strand, within PS417_07825 at 1718.972 kb on + strand, within PS417_07825 at 1718.973 kb on - strand, within PS417_07825 at 1719.054 kb on - strand, within PS417_07825 at 1719.106 kb on + strand at 1719.106 kb on + strand at 1719.106 kb on + strand at 1719.107 kb on - strand at 1719.119 kb on + strand at 1719.119 kb on + strand at 1719.120 kb on - strand at 1719.120 kb on - strand at 1719.285 kb on + strand at 1719.286 kb on - strand at 1719.387 kb on + strand, within PS417_07830 at 1719.388 kb on - strand, within PS417_07830 at 1719.409 kb on + strand, within PS417_07830 at 1719.410 kb on - strand, within PS417_07830 at 1719.410 kb on - strand, within PS417_07830 at 1719.410 kb on - strand, within PS417_07830 at 1719.433 kb on - strand, within PS417_07830 at 1719.433 kb on - strand, within PS417_07830 at 1719.541 kb on + strand, within PS417_07830 at 1719.542 kb on - strand, within PS417_07830 at 1719.606 kb on - strand, within PS417_07830
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,717,477 + PS417_07810 0.62 +0.2 1,717,477 + PS417_07810 0.62 +0.8 1,717,498 + PS417_07810 0.64 -0.1 1,717,617 + PS417_07810 0.78 +0.9 1,717,618 - PS417_07810 0.78 -0.3 1,717,618 - PS417_07810 0.78 -0.2 1,717,618 - PS417_07810 0.78 +0.1 1,717,763 + -0.1 1,717,993 + PS417_07815 0.12 +0.0 1,717,993 + PS417_07815 0.12 -0.9 1,718,100 + PS417_07815 0.59 -0.7 1,718,100 + PS417_07815 0.59 -0.6 1,718,100 + PS417_07815 0.59 +0.6 1,718,100 + PS417_07815 0.59 +0.9 1,718,100 + -2.1 1,718,145 + PS417_07815 0.79 -2.5 1,718,146 - PS417_07815 0.80 -1.6 1,718,169 - -0.5 1,718,192 - +0.1 1,718,204 + -0.5 1,718,204 + -0.1 1,718,204 + -0.4 1,718,205 - -0.9 1,718,205 - -1.3 1,718,211 - +0.1 1,718,273 + -0.1 1,718,274 - +0.2 1,718,274 - +2.3 1,718,274 - -1.4 1,718,274 - -1.0 1,718,284 + -1.0 1,718,284 + +0.7 1,718,285 - +0.5 1,718,285 - +1.1 1,718,285 - -1.1 1,718,293 - +0.4 1,718,326 + +0.8 1,718,326 + -0.0 1,718,326 + +0.4 1,718,326 + +0.3 1,718,326 + +1.1 1,718,326 + +1.2 1,718,326 + -1.4 1,718,326 + -0.2 1,718,327 - +0.6 1,718,327 - -0.2 1,718,327 - -0.7 1,718,327 - +0.5 1,718,327 - +0.2 1,718,327 - +1.6 1,718,354 + -1.0 1,718,437 + -0.8 1,718,437 + -0.1 1,718,438 - -0.2 1,718,438 - -0.6 1,718,438 - -0.4 1,718,640 + PS417_07820 0.58 -1.1 1,718,640 + PS417_07820 0.58 -1.1 1,718,641 - PS417_07820 0.58 -0.9 1,718,641 - PS417_07820 0.58 +0.9 1,718,790 + +1.9 1,718,790 + -0.1 1,718,790 + -0.2 1,718,791 - +1.1 1,718,791 - +0.2 1,718,890 + PS417_07825 0.35 +0.6 1,718,890 + PS417_07825 0.35 +0.8 1,718,890 + PS417_07825 0.35 -0.3 1,718,891 - PS417_07825 0.35 -2.2 1,718,891 - PS417_07825 0.35 +0.2 1,718,972 + PS417_07825 0.59 -1.3 1,718,973 - PS417_07825 0.60 -0.5 1,719,054 - PS417_07825 0.84 -0.1 1,719,106 + -0.3 1,719,106 + -0.4 1,719,106 + +0.2 1,719,107 - +1.6 1,719,119 + -0.1 1,719,119 + +0.2 1,719,120 - +0.8 1,719,120 - +1.6 1,719,285 + -0.6 1,719,286 - +1.3 1,719,387 + PS417_07830 0.14 +0.9 1,719,388 - PS417_07830 0.14 -0.3 1,719,409 + PS417_07830 0.16 -0.2 1,719,410 - PS417_07830 0.16 -0.3 1,719,410 - PS417_07830 0.16 -0.4 1,719,410 - PS417_07830 0.16 +0.2 1,719,433 - PS417_07830 0.17 +0.1 1,719,433 - PS417_07830 0.17 +0.6 1,719,541 + PS417_07830 0.24 +0.6 1,719,542 - PS417_07830 0.24 +0.1 1,719,606 - PS417_07830 0.28 +0.2
Or see this region's nucleotide sequence