Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07455 and PS417_07460 are separated by 11 nucleotides PS417_07460 and PS417_07465 are separated by 110 nucleotides PS417_07465 and PS417_07470 are separated by 176 nucleotides PS417_07470 and PS417_07475 overlap by 4 nucleotides
PS417_07455: PS417_07455 - glutamine amidotransferase, at 1,640,186 to 1,640,962
_07455
PS417_07460: PS417_07460 - hypothetical protein, at 1,640,974 to 1,641,516
_07460
PS417_07465: PS417_07465 - LysR family transcriptional regulator, at 1,641,627 to 1,642,565
_07465
PS417_07470: PS417_07470 - hypothetical protein, at 1,642,742 to 1,643,482
_07470
PS417_07475: PS417_07475 - allophanate hydrolase, at 1,643,479 to 1,644,183
_07475
Position (kb) 1641
1642
1643 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1640.836 kb on + strand, within PS417_07455 at 1640.960 kb on + strand at 1640.986 kb on + strand at 1641.578 kb on + strand at 1641.646 kb on - strand at 1641.991 kb on + strand, within PS417_07465 at 1641.992 kb on - strand, within PS417_07465 at 1642.046 kb on - strand at 1642.046 kb on - strand, within PS417_07465 at 1642.046 kb on - strand, within PS417_07465 at 1642.419 kb on + strand, within PS417_07465 at 1642.420 kb on - strand, within PS417_07465 at 1642.693 kb on + strand at 1642.708 kb on + strand at 1642.725 kb on + strand at 1643.063 kb on + strand, within PS417_07470 at 1643.217 kb on - strand, within PS417_07470 at 1643.223 kb on + strand, within PS417_07470 at 1643.415 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,640,836 + PS417_07455 0.84 -0.0 1,640,960 + -0.7 1,640,986 + +0.3 1,641,578 + +0.6 1,641,646 - -0.3 1,641,991 + PS417_07465 0.39 -1.5 1,641,992 - PS417_07465 0.39 -0.7 1,642,046 - +0.6 1,642,046 - PS417_07465 0.45 -0.2 1,642,046 - PS417_07465 0.45 -1.1 1,642,419 + PS417_07465 0.84 +1.6 1,642,420 - PS417_07465 0.84 -1.1 1,642,693 + -0.3 1,642,708 + +0.1 1,642,725 + -0.6 1,643,063 + PS417_07470 0.43 +2.6 1,643,217 - PS417_07470 0.64 -1.9 1,643,223 + PS417_07470 0.65 -0.4 1,643,415 + -0.3
Or see this region's nucleotide sequence