Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06020 and PS417_06025 are separated by 156 nucleotides PS417_06025 and PS417_06030 overlap by 4 nucleotides PS417_06030 and PS417_06035 are separated by 168 nucleotides
PS417_06020: PS417_06020 - membrane protein, at 1,337,971 to 1,339,248
_06020
PS417_06025: PS417_06025 - alpha/beta hydrolase, at 1,339,405 to 1,340,310
_06025
PS417_06030: PS417_06030 - hypothetical protein, at 1,340,307 to 1,340,633
_06030
PS417_06035: PS417_06035 - hypothetical protein, at 1,340,802 to 1,341,146
_06035
Position (kb)
1339
1340
1341 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1338.525 kb on + strand, within PS417_06020 at 1338.526 kb on - strand, within PS417_06020 at 1338.526 kb on - strand, within PS417_06020 at 1338.581 kb on - strand, within PS417_06020 at 1338.997 kb on + strand, within PS417_06020 at 1338.998 kb on - strand, within PS417_06020 at 1339.003 kb on + strand, within PS417_06020 at 1339.003 kb on + strand, within PS417_06020 at 1339.004 kb on - strand, within PS417_06020 at 1339.004 kb on - strand, within PS417_06020 at 1339.132 kb on + strand at 1339.133 kb on - strand at 1339.303 kb on + strand at 1339.304 kb on - strand at 1339.304 kb on - strand at 1339.304 kb on - strand at 1339.304 kb on - strand at 1339.304 kb on - strand at 1339.305 kb on + strand at 1339.305 kb on + strand at 1339.305 kb on + strand at 1339.305 kb on + strand at 1339.305 kb on + strand at 1339.305 kb on + strand at 1339.305 kb on + strand at 1339.305 kb on + strand at 1339.305 kb on + strand at 1339.306 kb on - strand at 1339.306 kb on - strand at 1339.306 kb on - strand at 1339.306 kb on - strand at 1339.306 kb on - strand at 1339.306 kb on - strand at 1339.306 kb on - strand at 1339.306 kb on - strand at 1339.306 kb on - strand at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.309 kb on + strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.310 kb on - strand at 1339.385 kb on + strand at 1339.386 kb on - strand at 1339.389 kb on - strand at 1339.433 kb on + strand at 1339.516 kb on + strand, within PS417_06025 at 1339.711 kb on + strand, within PS417_06025 at 1339.751 kb on - strand, within PS417_06025 at 1339.859 kb on - strand, within PS417_06025 at 1339.859 kb on - strand, within PS417_06025 at 1339.938 kb on - strand, within PS417_06025 at 1339.938 kb on - strand, within PS417_06025 at 1340.269 kb on + strand at 1340.270 kb on - strand at 1340.280 kb on + strand at 1340.389 kb on - strand, within PS417_06030 at 1340.389 kb on - strand, within PS417_06030 at 1340.394 kb on + strand, within PS417_06030 at 1340.473 kb on - strand, within PS417_06030 at 1340.473 kb on - strand, within PS417_06030 at 1340.687 kb on + strand at 1340.800 kb on - strand at 1340.800 kb on - strand at 1340.800 kb on - strand at 1340.800 kb on - strand at 1340.800 kb on - strand at 1340.802 kb on - strand at 1340.810 kb on + strand at 1340.915 kb on + strand, within PS417_06035 at 1340.927 kb on + strand, within PS417_06035 at 1341.201 kb on - strand at 1341.201 kb on - strand at 1341.288 kb on + strand at 1341.289 kb on - strand at 1341.289 kb on - strand at 1341.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,338,525 + PS417_06020 0.43 -0.8 1,338,526 - PS417_06020 0.43 -2.2 1,338,526 - PS417_06020 0.43 -0.1 1,338,581 - PS417_06020 0.48 +1.0 1,338,997 + PS417_06020 0.80 -0.5 1,338,998 - PS417_06020 0.80 -0.3 1,339,003 + PS417_06020 0.81 +1.4 1,339,003 + PS417_06020 0.81 -1.3 1,339,004 - PS417_06020 0.81 +0.7 1,339,004 - PS417_06020 0.81 +0.0 1,339,132 + -0.7 1,339,133 - +1.5 1,339,303 + -1.1 1,339,304 - -1.6 1,339,304 - -1.3 1,339,304 - -0.5 1,339,304 - -0.4 1,339,304 - -0.5 1,339,305 + -0.7 1,339,305 + -0.0 1,339,305 + +0.1 1,339,305 + -1.1 1,339,305 + +1.1 1,339,305 + +0.3 1,339,305 + +0.2 1,339,305 + +1.4 1,339,305 + -1.8 1,339,306 - +0.1 1,339,306 - -0.1 1,339,306 - -0.7 1,339,306 - +0.8 1,339,306 - -0.4 1,339,306 - +4.7 1,339,306 - +0.4 1,339,306 - -0.8 1,339,306 - -1.6 1,339,309 + -0.1 1,339,309 + +0.7 1,339,309 + -0.1 1,339,309 + -0.3 1,339,309 + -0.4 1,339,309 + -0.4 1,339,309 + -1.9 1,339,310 - -0.4 1,339,310 - -0.3 1,339,310 - -0.1 1,339,310 - +1.0 1,339,310 - -0.4 1,339,310 - -1.1 1,339,310 - +0.5 1,339,310 - +2.6 1,339,310 - +0.0 1,339,385 + +1.9 1,339,386 - -0.1 1,339,389 - -1.6 1,339,433 + -0.5 1,339,516 + PS417_06025 0.12 +1.5 1,339,711 + PS417_06025 0.34 +0.2 1,339,751 - PS417_06025 0.38 -2.1 1,339,859 - PS417_06025 0.50 -1.6 1,339,859 - PS417_06025 0.50 -1.9 1,339,938 - PS417_06025 0.59 +0.5 1,339,938 - PS417_06025 0.59 -2.1 1,340,269 + -2.8 1,340,270 - -0.7 1,340,280 + +0.4 1,340,389 - PS417_06030 0.25 +0.5 1,340,389 - PS417_06030 0.25 -0.5 1,340,394 + PS417_06030 0.27 -0.5 1,340,473 - PS417_06030 0.51 -1.1 1,340,473 - PS417_06030 0.51 -0.5 1,340,687 + -0.8 1,340,800 - +0.8 1,340,800 - -1.5 1,340,800 - +0.4 1,340,800 - -1.4 1,340,800 - -0.8 1,340,802 - -0.3 1,340,810 + -1.2 1,340,915 + PS417_06035 0.33 +1.5 1,340,927 + PS417_06035 0.36 +0.9 1,341,201 - -1.2 1,341,201 - +1.5 1,341,288 + +0.5 1,341,289 - -1.1 1,341,289 - -0.1 1,341,300 - -0.6
Or see this region's nucleotide sequence