Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05645 and PS417_05650 are separated by 84 nucleotides PS417_05650 and PS417_05655 are separated by 107 nucleotides PS417_05655 and PS417_05660 are separated by 65 nucleotides
PS417_05645: PS417_05645 - LuxR family transcriptional regulator, at 1,269,663 to 1,270,295
_05645
PS417_05650: PS417_05650 - leucyl-tRNA synthetase, at 1,270,380 to 1,270,562
_05650
PS417_05655: PS417_05655 - endonuclease III, at 1,270,670 to 1,271,308
_05655
PS417_05660: PS417_05660 - (4Fe-4S)-binding protein, at 1,271,374 to 1,272,327
_05660
Position (kb)
1270
1271
1272 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1269.716 kb on + strand at 1269.815 kb on - strand, within PS417_05645 at 1269.854 kb on + strand, within PS417_05645 at 1269.854 kb on + strand, within PS417_05645 at 1269.873 kb on - strand, within PS417_05645 at 1270.035 kb on + strand, within PS417_05645 at 1270.035 kb on + strand, within PS417_05645 at 1270.036 kb on - strand, within PS417_05645 at 1270.036 kb on - strand, within PS417_05645 at 1270.133 kb on - strand, within PS417_05645 at 1270.197 kb on - strand, within PS417_05645 at 1270.212 kb on + strand, within PS417_05645 at 1270.213 kb on - strand, within PS417_05645 at 1270.213 kb on - strand, within PS417_05645 at 1270.292 kb on - strand at 1270.294 kb on - strand at 1270.294 kb on - strand at 1270.308 kb on + strand at 1270.308 kb on + strand at 1270.309 kb on - strand at 1270.309 kb on - strand at 1270.309 kb on - strand at 1270.309 kb on - strand at 1270.309 kb on - strand at 1270.445 kb on + strand, within PS417_05650 at 1270.445 kb on + strand, within PS417_05650 at 1270.500 kb on + strand, within PS417_05650 at 1270.500 kb on + strand, within PS417_05650 at 1270.501 kb on - strand at 1270.501 kb on - strand, within PS417_05650 at 1270.562 kb on + strand at 1270.562 kb on + strand at 1270.562 kb on + strand at 1270.563 kb on - strand at 1270.580 kb on - strand at 1270.601 kb on + strand at 1270.602 kb on - strand at 1270.604 kb on - strand at 1270.604 kb on - strand at 1270.755 kb on + strand, within PS417_05655 at 1270.755 kb on + strand, within PS417_05655 at 1270.756 kb on - strand at 1270.889 kb on - strand, within PS417_05655 at 1270.889 kb on - strand, within PS417_05655 at 1271.065 kb on - strand, within PS417_05655 at 1271.086 kb on - strand, within PS417_05655 at 1271.115 kb on + strand, within PS417_05655 at 1271.115 kb on + strand, within PS417_05655 at 1271.115 kb on + strand, within PS417_05655 at 1271.115 kb on + strand at 1271.347 kb on - strand at 1271.347 kb on - strand at 1271.416 kb on + strand at 1271.416 kb on + strand at 1271.417 kb on - strand at 1271.417 kb on - strand at 1271.529 kb on + strand at 1271.676 kb on + strand, within PS417_05660 at 1271.676 kb on + strand, within PS417_05660 at 1271.677 kb on - strand, within PS417_05660 at 1271.677 kb on - strand, within PS417_05660 at 1271.742 kb on + strand, within PS417_05660 at 1271.759 kb on - strand, within PS417_05660 at 1271.835 kb on + strand, within PS417_05660 at 1271.835 kb on + strand, within PS417_05660 at 1271.836 kb on - strand, within PS417_05660 at 1271.836 kb on - strand, within PS417_05660 at 1271.836 kb on - strand, within PS417_05660 at 1271.926 kb on + strand, within PS417_05660 at 1271.927 kb on - strand, within PS417_05660 at 1272.105 kb on - strand, within PS417_05660
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,269,716 + +0.1 1,269,815 - PS417_05645 0.24 -0.3 1,269,854 + PS417_05645 0.30 +0.4 1,269,854 + PS417_05645 0.30 -0.1 1,269,873 - PS417_05645 0.33 -0.1 1,270,035 + PS417_05645 0.59 -0.4 1,270,035 + PS417_05645 0.59 -1.2 1,270,036 - PS417_05645 0.59 -0.1 1,270,036 - PS417_05645 0.59 +0.5 1,270,133 - PS417_05645 0.74 +1.9 1,270,197 - PS417_05645 0.84 -0.9 1,270,212 + PS417_05645 0.87 +0.1 1,270,213 - PS417_05645 0.87 -0.2 1,270,213 - PS417_05645 0.87 +0.0 1,270,292 - +1.0 1,270,294 - -0.1 1,270,294 - -0.4 1,270,308 + -0.1 1,270,308 + -0.6 1,270,309 - -0.2 1,270,309 - -0.3 1,270,309 - +2.7 1,270,309 - -0.2 1,270,309 - +0.7 1,270,445 + PS417_05650 0.36 +0.0 1,270,445 + PS417_05650 0.36 -1.1 1,270,500 + PS417_05650 0.66 +0.9 1,270,500 + PS417_05650 0.66 -1.7 1,270,501 - -0.1 1,270,501 - PS417_05650 0.66 -0.3 1,270,562 + -0.7 1,270,562 + +1.2 1,270,562 + -0.0 1,270,563 - -1.3 1,270,580 - -0.3 1,270,601 + -0.5 1,270,602 - -0.9 1,270,604 - -0.7 1,270,604 - -0.7 1,270,755 + PS417_05655 0.13 +0.1 1,270,755 + PS417_05655 0.13 -1.6 1,270,756 - +1.5 1,270,889 - PS417_05655 0.34 +0.2 1,270,889 - PS417_05655 0.34 +0.1 1,271,065 - PS417_05655 0.62 +0.6 1,271,086 - PS417_05655 0.65 -2.4 1,271,115 + PS417_05655 0.70 +0.1 1,271,115 + PS417_05655 0.70 +1.8 1,271,115 + PS417_05655 0.70 +0.4 1,271,115 + +1.5 1,271,347 - +0.5 1,271,347 - -0.1 1,271,416 + +1.1 1,271,416 + -0.4 1,271,417 - -0.5 1,271,417 - +0.1 1,271,529 + -1.1 1,271,676 + PS417_05660 0.32 -0.5 1,271,676 + PS417_05660 0.32 -0.8 1,271,677 - PS417_05660 0.32 -0.2 1,271,677 - PS417_05660 0.32 -1.1 1,271,742 + PS417_05660 0.39 +0.9 1,271,759 - PS417_05660 0.40 -0.8 1,271,835 + PS417_05660 0.48 +0.0 1,271,835 + PS417_05660 0.48 -0.7 1,271,836 - PS417_05660 0.48 +1.9 1,271,836 - PS417_05660 0.48 -0.1 1,271,836 - PS417_05660 0.48 +2.9 1,271,926 + PS417_05660 0.58 -0.3 1,271,927 - PS417_05660 0.58 -0.0 1,272,105 - PS417_05660 0.77 -0.1
Or see this region's nucleotide sequence