Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02995 and PS417_03000 are separated by 182 nucleotides PS417_03000 and PS417_03005 are separated by 142 nucleotides PS417_03005 and PS417_03010 are separated by 131 nucleotides
PS417_02995: PS417_02995 - NUDIX hydrolase, at 685,557 to 686,093
_02995
PS417_03000: PS417_03000 - translation initiation factor Sui1, at 686,276 to 686,647
_03000
PS417_03005: PS417_03005 - arginine decarboxylase, at 686,790 to 688,703
_03005
PS417_03010: PS417_03010 - XRE family transcriptional regulator, at 688,835 to 690,178
_03010
Position (kb) 686
687
688
689 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 685.819 kb on - strand, within PS417_02995 at 685.897 kb on - strand, within PS417_02995 at 685.928 kb on + strand, within PS417_02995 at 685.928 kb on + strand, within PS417_02995 at 685.929 kb on - strand, within PS417_02995 at 685.999 kb on + strand, within PS417_02995 at 685.999 kb on + strand, within PS417_02995 at 686.040 kb on + strand at 686.041 kb on - strand at 686.041 kb on - strand at 686.102 kb on - strand at 686.102 kb on - strand at 686.124 kb on - strand at 686.145 kb on + strand at 686.320 kb on - strand, within PS417_03000 at 686.320 kb on - strand, within PS417_03000 at 686.378 kb on - strand, within PS417_03000 at 686.378 kb on - strand, within PS417_03000 at 686.621 kb on + strand at 686.621 kb on + strand at 686.621 kb on + strand at 686.731 kb on + strand at 686.731 kb on + strand at 686.776 kb on + strand at 686.776 kb on + strand at 686.776 kb on + strand at 686.777 kb on - strand at 687.038 kb on - strand, within PS417_03005 at 687.097 kb on - strand, within PS417_03005 at 687.586 kb on - strand, within PS417_03005 at 687.687 kb on + strand, within PS417_03005 at 688.008 kb on + strand, within PS417_03005 at 688.008 kb on + strand, within PS417_03005 at 688.009 kb on - strand, within PS417_03005 at 688.083 kb on + strand, within PS417_03005 at 688.083 kb on + strand, within PS417_03005 at 688.084 kb on - strand, within PS417_03005 at 688.110 kb on - strand, within PS417_03005 at 688.182 kb on + strand, within PS417_03005 at 688.182 kb on + strand, within PS417_03005 at 688.182 kb on + strand, within PS417_03005 at 688.183 kb on - strand, within PS417_03005 at 688.339 kb on - strand, within PS417_03005 at 688.401 kb on + strand, within PS417_03005 at 688.401 kb on + strand, within PS417_03005 at 688.401 kb on + strand, within PS417_03005 at 688.401 kb on + strand, within PS417_03005 at 688.431 kb on + strand, within PS417_03005 at 688.451 kb on + strand, within PS417_03005 at 688.491 kb on + strand, within PS417_03005 at 688.574 kb on - strand at 688.702 kb on - strand at 688.702 kb on - strand at 688.829 kb on - strand at 688.840 kb on + strand at 688.840 kb on + strand at 688.841 kb on - strand at 688.843 kb on + strand at 688.843 kb on + strand at 688.844 kb on - strand at 688.847 kb on + strand at 688.868 kb on + strand at 689.059 kb on - strand, within PS417_03010 at 689.095 kb on - strand, within PS417_03010 at 689.095 kb on - strand, within PS417_03010 at 689.103 kb on + strand, within PS417_03010 at 689.104 kb on - strand, within PS417_03010 at 689.104 kb on - strand, within PS417_03010 at 689.152 kb on + strand, within PS417_03010 at 689.153 kb on - strand, within PS417_03010 at 689.330 kb on + strand, within PS417_03010 at 689.330 kb on + strand, within PS417_03010 at 689.330 kb on + strand, within PS417_03010
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 685,819 - PS417_02995 0.49 -1.7 685,897 - PS417_02995 0.63 +0.6 685,928 + PS417_02995 0.69 +0.1 685,928 + PS417_02995 0.69 -0.1 685,929 - PS417_02995 0.69 -0.2 685,999 + PS417_02995 0.82 -0.7 685,999 + PS417_02995 0.82 -0.7 686,040 + +0.7 686,041 - -0.3 686,041 - +0.1 686,102 - +0.0 686,102 - +0.2 686,124 - -0.4 686,145 + -0.1 686,320 - PS417_03000 0.12 -0.9 686,320 - PS417_03000 0.12 +0.0 686,378 - PS417_03000 0.27 -0.7 686,378 - PS417_03000 0.27 -0.7 686,621 + -1.2 686,621 + -0.1 686,621 + +0.1 686,731 + -0.1 686,731 + +0.7 686,776 + -0.1 686,776 + -0.2 686,776 + +0.4 686,777 - -0.5 687,038 - PS417_03005 0.13 -0.8 687,097 - PS417_03005 0.16 -0.2 687,586 - PS417_03005 0.42 -0.8 687,687 + PS417_03005 0.47 -1.2 688,008 + PS417_03005 0.64 -1.0 688,008 + PS417_03005 0.64 -1.0 688,009 - PS417_03005 0.64 +1.2 688,083 + PS417_03005 0.68 -0.1 688,083 + PS417_03005 0.68 -1.9 688,084 - PS417_03005 0.68 -0.3 688,110 - PS417_03005 0.69 -1.3 688,182 + PS417_03005 0.73 -3.1 688,182 + PS417_03005 0.73 -1.0 688,182 + PS417_03005 0.73 -1.4 688,183 - PS417_03005 0.73 -2.9 688,339 - PS417_03005 0.81 -0.6 688,401 + PS417_03005 0.84 -0.7 688,401 + PS417_03005 0.84 -0.8 688,401 + PS417_03005 0.84 +0.2 688,401 + PS417_03005 0.84 -0.7 688,431 + PS417_03005 0.86 +0.1 688,451 + PS417_03005 0.87 -0.6 688,491 + PS417_03005 0.89 +0.6 688,574 - -1.5 688,702 - -0.3 688,702 - -0.1 688,829 - -0.6 688,840 + +0.0 688,840 + +0.4 688,841 - -0.5 688,843 + -0.1 688,843 + +0.9 688,844 - +0.4 688,847 + -0.5 688,868 + +0.8 689,059 - PS417_03010 0.17 -0.4 689,095 - PS417_03010 0.19 +0.6 689,095 - PS417_03010 0.19 +0.0 689,103 + PS417_03010 0.20 +0.3 689,104 - PS417_03010 0.20 +0.8 689,104 - PS417_03010 0.20 +3.6 689,152 + PS417_03010 0.24 -0.6 689,153 - PS417_03010 0.24 -0.8 689,330 + PS417_03010 0.37 -0.6 689,330 + PS417_03010 0.37 +0.0 689,330 + PS417_03010 0.37 +0.0
Or see this region's nucleotide sequence