Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24230 and PS417_24235 are separated by 267 nucleotides PS417_24235 and PS417_24240 are separated by 91 nucleotides
PS417_24230: PS417_24230 - ATP-dependent protease, at 5,255,068 to 5,257,506
_24230
PS417_24235: PS417_24235 - hypothetical protein, at 5,257,774 to 5,258,262
_24235
PS417_24240: PS417_24240 - membrane protein, at 5,258,354 to 5,260,207
_24240
Position (kb)
5257
5258
5259 Strain fitness (log2 ratio)
-2
-1
0
1 at 5257.223 kb on + strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.224 kb on - strand, within PS417_24230 at 5257.517 kb on + strand at 5257.518 kb on - strand at 5257.518 kb on - strand at 5257.673 kb on + strand at 5257.674 kb on - strand at 5257.699 kb on + strand at 5257.699 kb on + strand at 5257.700 kb on - strand at 5257.700 kb on - strand at 5257.703 kb on - strand at 5257.703 kb on - strand at 5257.774 kb on - strand at 5257.926 kb on + strand, within PS417_24235 at 5257.927 kb on - strand, within PS417_24235 at 5257.997 kb on - strand, within PS417_24235 at 5258.087 kb on - strand, within PS417_24235 at 5258.087 kb on - strand, within PS417_24235 at 5258.260 kb on + strand at 5258.261 kb on - strand at 5258.331 kb on + strand at 5258.342 kb on + strand at 5258.343 kb on - strand at 5258.354 kb on - strand at 5258.510 kb on + strand at 5258.510 kb on + strand at 5258.510 kb on + strand at 5258.510 kb on + strand at 5258.511 kb on - strand at 5258.511 kb on - strand at 5258.511 kb on - strand at 5258.511 kb on - strand at 5258.880 kb on - strand, within PS417_24240 at 5259.122 kb on + strand, within PS417_24240 at 5259.237 kb on + strand, within PS417_24240
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 5,257,223 + PS417_24230 0.88 -1.4 5,257,224 - PS417_24230 0.88 -0.0 5,257,224 - PS417_24230 0.88 -0.9 5,257,224 - PS417_24230 0.88 -0.4 5,257,224 - PS417_24230 0.88 +0.1 5,257,224 - PS417_24230 0.88 -0.2 5,257,517 + -0.2 5,257,518 - -0.3 5,257,518 - +0.1 5,257,673 + +1.6 5,257,674 - -1.0 5,257,699 + -0.1 5,257,699 + -2.4 5,257,700 - +0.6 5,257,700 - -1.4 5,257,703 - -0.8 5,257,703 - -1.4 5,257,774 - -0.0 5,257,926 + PS417_24235 0.31 -1.3 5,257,927 - PS417_24235 0.31 -2.7 5,257,997 - PS417_24235 0.46 -0.9 5,258,087 - PS417_24235 0.64 -1.7 5,258,087 - PS417_24235 0.64 +0.2 5,258,260 + +0.1 5,258,261 - -0.8 5,258,331 + +0.1 5,258,342 + +1.3 5,258,343 - +0.3 5,258,354 - +0.2 5,258,510 + -0.3 5,258,510 + -0.3 5,258,510 + -0.7 5,258,510 + +0.1 5,258,511 - -0.7 5,258,511 - -1.1 5,258,511 - +1.6 5,258,511 - -1.0 5,258,880 - PS417_24240 0.28 +1.1 5,259,122 + PS417_24240 0.41 -1.9 5,259,237 + PS417_24240 0.48 +0.6
Or see this region's nucleotide sequence