Strain Fitness in Pseudomonas simiae WCS417 around PS417_02225

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_02220 and PS417_02225 are separated by 383 nucleotidesPS417_02225 and PS417_02230 are separated by 54 nucleotides PS417_02220: PS417_02220 - pyruvate dehydrogenase, at 502,327 to 504,972 _02220 PS417_02225: PS417_02225 - bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase, at 505,356 to 508,295 _02225 PS417_02230: PS417_02230 - ADP-heptose--LPS heptosyltransferase, at 508,350 to 509,384 _02230 Position (kb) 505 506 507 508 509Strain fitness (log2 ratio) -1 0 1at 505.128 kb on - strandat 505.132 kb on - strandat 505.138 kb on - strandat 505.297 kb on - strandat 505.311 kb on - strandat 505.323 kb on - strandat 506.676 kb on + strand, within PS417_02225at 506.677 kb on - strand, within PS417_02225at 507.039 kb on + strand, within PS417_02225at 508.301 kb on + strandat 508.319 kb on + strandat 508.319 kb on + strandat 508.347 kb on + strandat 508.347 kb on + strandat 508.347 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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505,128 - -0.7
505,132 - -1.1
505,138 - -0.3
505,297 - -0.5
505,311 - +1.2
505,323 - -1.0
506,676 + PS417_02225 0.45 -0.1
506,677 - PS417_02225 0.45 -1.2
507,039 + PS417_02225 0.57 +0.6
508,301 + +0.6
508,319 + -0.7
508,319 + -0.0
508,347 + +0.3
508,347 + +1.1
508,347 + +0.8

Or see this region's nucleotide sequence