Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02145 and PS417_02150 are separated by 50 nucleotides PS417_02150 and PS417_02155 are separated by 243 nucleotides
PS417_02145: PS417_02145 - potassium transporter KefA, at 477,221 to 480,559
_02145
PS417_02150: PS417_02150 - potassium/proton antiporter, at 480,610 to 482,352
_02150
PS417_02155: PS417_02155 - chemotaxis protein, at 482,596 to 484,221
_02155
Position (kb) 480
481
482
483 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 479.712 kb on + strand, within PS417_02145 at 479.713 kb on - strand, within PS417_02145 at 479.891 kb on + strand, within PS417_02145 at 479.891 kb on + strand, within PS417_02145 at 479.891 kb on + strand, within PS417_02145 at 479.892 kb on - strand, within PS417_02145 at 480.373 kb on - strand at 480.373 kb on - strand at 480.373 kb on - strand at 480.554 kb on - strand at 480.559 kb on + strand at 480.559 kb on + strand at 480.611 kb on + strand at 480.982 kb on - strand, within PS417_02150 at 481.265 kb on + strand, within PS417_02150 at 481.266 kb on - strand, within PS417_02150 at 481.350 kb on + strand, within PS417_02150 at 481.471 kb on + strand, within PS417_02150 at 481.472 kb on - strand, within PS417_02150 at 481.513 kb on - strand, within PS417_02150 at 481.513 kb on - strand, within PS417_02150 at 481.548 kb on + strand, within PS417_02150 at 481.549 kb on - strand, within PS417_02150 at 481.689 kb on - strand, within PS417_02150 at 481.689 kb on - strand, within PS417_02150 at 481.963 kb on - strand, within PS417_02150 at 482.187 kb on + strand at 482.251 kb on + strand at 482.252 kb on - strand at 482.253 kb on + strand at 482.253 kb on + strand at 482.254 kb on - strand at 482.254 kb on - strand at 482.359 kb on + strand at 482.420 kb on + strand at 482.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 479,712 + PS417_02145 0.75 -2.2 479,713 - PS417_02145 0.75 -2.7 479,891 + PS417_02145 0.80 -2.7 479,891 + PS417_02145 0.80 -1.8 479,891 + PS417_02145 0.80 -1.7 479,892 - PS417_02145 0.80 -2.6 480,373 - +5.3 480,373 - -1.6 480,373 - -1.5 480,554 - -1.0 480,559 + -1.0 480,559 + -1.2 480,611 + -0.3 480,982 - PS417_02150 0.21 -1.1 481,265 + PS417_02150 0.38 -1.1 481,266 - PS417_02150 0.38 -0.3 481,350 + PS417_02150 0.42 -1.1 481,471 + PS417_02150 0.49 -2.8 481,472 - PS417_02150 0.49 +0.3 481,513 - PS417_02150 0.52 -1.8 481,513 - PS417_02150 0.52 +0.3 481,548 + PS417_02150 0.54 -1.8 481,549 - PS417_02150 0.54 -0.4 481,689 - PS417_02150 0.62 -0.6 481,689 - PS417_02150 0.62 -0.8 481,963 - PS417_02150 0.78 +0.4 482,187 + -1.3 482,251 + -1.3 482,252 - -0.0 482,253 + -1.4 482,253 + -0.4 482,254 - -1.2 482,254 - -1.4 482,359 + -0.3 482,420 + -0.6 482,598 - -0.6
Or see this region's nucleotide sequence