Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01905 and PS417_01910 are separated by 249 nucleotides PS417_01910 and PS417_01915 are separated by 148 nucleotides PS417_01915 and PS417_01920 are separated by 36 nucleotides PS417_01920 and PS417_01925 are separated by 257 nucleotides
PS417_01905: PS417_01905 - poly(R)-hydroxyalkanoic acid synthase, at 421,219 to 422,898
_01905
PS417_01910: PS417_01910 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, at 423,148 to 423,525
_01910
PS417_01915: PS417_01915 - ATP-dependent protease ATP-binding subunit HslU, at 423,674 to 425,011
_01915
PS417_01920: PS417_01920 - ATP-dependent protease subunit HslV, at 425,048 to 425,578
_01920
PS417_01925: PS417_01925 - cell division protein, at 425,836 to 426,537
_01925
Position (kb) 423
424
425
426 Strain fitness (log2 ratio) -1
0
1 at 422.892 kb on + strand at 422.893 kb on - strand at 422.901 kb on - strand at 423.599 kb on + strand at 423.676 kb on - strand at 424.743 kb on + strand, within PS417_01915 at 425.012 kb on - strand at 425.012 kb on - strand at 425.464 kb on - strand, within PS417_01920 at 425.684 kb on + strand at 425.684 kb on + strand at 425.684 kb on + strand at 425.685 kb on - strand at 425.764 kb on + strand at 425.765 kb on - strand at 425.781 kb on - strand at 425.781 kb on - strand at 425.787 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 422,892 + +0.0 422,893 - +0.4 422,901 - -0.0 423,599 + +0.2 423,676 - +0.1 424,743 + PS417_01915 0.80 -1.2 425,012 - +0.6 425,012 - +0.3 425,464 - PS417_01920 0.78 -0.6 425,684 + +0.1 425,684 + +0.1 425,684 + -0.9 425,685 - -0.3 425,764 + -1.1 425,765 - -0.2 425,781 - -0.4 425,781 - -1.3 425,787 + +0.3
Or see this region's nucleotide sequence