Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17730 and PS417_17735 are separated by 63 nucleotides PS417_17735 and PS417_17740 are separated by 294 nucleotides PS417_17740 and PS417_17745 overlap by 4 nucleotides
PS417_17730: PS417_17730 - ribonucleotide-diphosphate reductase subunit alpha, at 3,869,663 to 3,871,171
_17730
PS417_17735: PS417_17735 - LacI family transcriptional regulator, at 3,871,235 to 3,872,158
_17735
PS417_17740: PS417_17740 - fatty acid hydroxylase, at 3,872,453 to 3,873,130
_17740
PS417_17745: PS417_17745 - gluconolactonase, at 3,873,127 to 3,874,227
_17745
Position (kb)
3871
3872
3873 Strain fitness (log2 ratio)
-1
0
1 at 3871.732 kb on - strand, within PS417_17735 at 3871.732 kb on - strand, within PS417_17735 at 3872.029 kb on - strand, within PS417_17735 at 3872.278 kb on + strand at 3872.367 kb on + strand at 3872.368 kb on - strand at 3872.368 kb on - strand at 3872.368 kb on - strand at 3872.414 kb on - strand at 3872.414 kb on - strand at 3872.591 kb on - strand, within PS417_17740 at 3872.591 kb on - strand, within PS417_17740 at 3872.591 kb on - strand, within PS417_17740 at 3872.591 kb on - strand, within PS417_17740 at 3872.593 kb on - strand, within PS417_17740 at 3872.593 kb on - strand, within PS417_17740 at 3872.705 kb on + strand, within PS417_17740 at 3873.071 kb on + strand at 3873.072 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,871,732 - PS417_17735 0.54 -1.2 3,871,732 - PS417_17735 0.54 -1.4 3,872,029 - PS417_17735 0.86 -0.4 3,872,278 + -0.7 3,872,367 + -0.1 3,872,368 - -1.2 3,872,368 - +0.5 3,872,368 - -0.7 3,872,414 - +0.7 3,872,414 - -0.1 3,872,591 - PS417_17740 0.20 -0.1 3,872,591 - PS417_17740 0.20 -1.4 3,872,591 - PS417_17740 0.20 -1.7 3,872,591 - PS417_17740 0.20 +0.9 3,872,593 - PS417_17740 0.21 -0.2 3,872,593 - PS417_17740 0.21 +0.2 3,872,705 + PS417_17740 0.37 -0.3 3,873,071 + -0.1 3,873,072 - +0.7
Or see this region's nucleotide sequence