Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14970 and PS417_14975 overlap by 45 nucleotides PS417_14975 and PS417_14980 are separated by 151 nucleotides PS417_14980 and PS417_14985 are separated by 131 nucleotides
PS417_14970: PS417_14970 - MFS transporter, at 3,272,620 to 3,273,819
_14970
PS417_14975: PS417_14975 - tRNA hydroxylase, at 3,273,775 to 3,274,377
_14975
PS417_14980: PS417_14980 - universal stress protein UspA, at 3,274,529 to 3,275,392
_14980
PS417_14985: PS417_14985 - Fe-S protein, at 3,275,524 to 3,275,997
_14985
Position (kb)
3274
3275
3276 Strain fitness (log2 ratio)
-1
0
1 at 3273.529 kb on + strand, within PS417_14970 at 3273.530 kb on - strand, within PS417_14970 at 3273.714 kb on + strand at 3273.893 kb on + strand, within PS417_14975 at 3273.908 kb on + strand, within PS417_14975 at 3274.097 kb on + strand, within PS417_14975 at 3274.223 kb on + strand, within PS417_14975 at 3274.223 kb on + strand, within PS417_14975 at 3274.416 kb on + strand at 3274.489 kb on + strand at 3274.489 kb on + strand at 3274.490 kb on - strand at 3274.879 kb on + strand, within PS417_14980 at 3275.539 kb on + strand at 3275.565 kb on + strand at 3275.567 kb on + strand at 3275.672 kb on + strand, within PS417_14985 at 3275.720 kb on + strand, within PS417_14985 at 3275.720 kb on + strand, within PS417_14985 at 3275.733 kb on - strand, within PS417_14985 at 3275.883 kb on - strand, within PS417_14985 at 3276.085 kb on + strand at 3276.086 kb on - strand at 3276.125 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,273,529 + PS417_14970 0.76 -1.1 3,273,530 - PS417_14970 0.76 -0.1 3,273,714 + -0.5 3,273,893 + PS417_14975 0.20 +0.9 3,273,908 + PS417_14975 0.22 -0.0 3,274,097 + PS417_14975 0.53 -0.1 3,274,223 + PS417_14975 0.74 -0.6 3,274,223 + PS417_14975 0.74 +0.1 3,274,416 + -1.7 3,274,489 + +0.1 3,274,489 + +0.5 3,274,490 - -0.4 3,274,879 + PS417_14980 0.41 -1.4 3,275,539 + -0.9 3,275,565 + +0.4 3,275,567 + +0.5 3,275,672 + PS417_14985 0.31 +0.9 3,275,720 + PS417_14985 0.41 -0.6 3,275,720 + PS417_14985 0.41 -1.7 3,275,733 - PS417_14985 0.44 -0.9 3,275,883 - PS417_14985 0.76 -0.5 3,276,085 + -0.3 3,276,086 - -0.5 3,276,125 + +1.2
Or see this region's nucleotide sequence