Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14630 and PS417_14635 overlap by 4 nucleotides PS417_14635 and PS417_14640 are separated by 385 nucleotides
PS417_14630: PS417_14630 - 3-demethylubiquinone-9 3-methyltransferase, at 3,195,413 to 3,195,895
_14630
PS417_14635: PS417_14635 - GDSL family lipase, at 3,195,892 to 3,196,788
_14635
PS417_14640: PS417_14640 - dihydrodipicolinate synthase, at 3,197,174 to 3,198,037
_14640
Position (kb)
3195
3196
3197 Strain fitness (log2 ratio)
-2
-1
0
1 at 3194.959 kb on + strand at 3194.959 kb on + strand at 3194.960 kb on - strand at 3195.014 kb on + strand at 3195.014 kb on + strand at 3195.015 kb on - strand at 3195.050 kb on + strand at 3195.051 kb on - strand at 3195.103 kb on + strand at 3195.106 kb on + strand at 3195.171 kb on + strand at 3195.217 kb on + strand at 3195.217 kb on + strand at 3195.217 kb on + strand at 3195.318 kb on + strand at 3195.319 kb on - strand at 3195.378 kb on - strand at 3195.559 kb on - strand, within PS417_14630 at 3195.965 kb on + strand at 3195.968 kb on + strand at 3195.968 kb on + strand at 3195.968 kb on + strand at 3195.968 kb on + strand at 3196.100 kb on + strand, within PS417_14635 at 3196.100 kb on + strand, within PS417_14635 at 3196.166 kb on + strand, within PS417_14635 at 3196.386 kb on - strand, within PS417_14635 at 3196.487 kb on + strand, within PS417_14635 at 3196.488 kb on - strand, within PS417_14635 at 3196.488 kb on - strand, within PS417_14635 at 3196.718 kb on + strand at 3196.719 kb on - strand at 3196.813 kb on + strand at 3196.880 kb on + strand at 3196.915 kb on + strand at 3197.191 kb on + strand at 3197.191 kb on + strand at 3197.192 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 3,194,959 + -0.1 3,194,959 + -0.9 3,194,960 - -0.4 3,195,014 + -1.8 3,195,014 + +1.5 3,195,015 - -0.7 3,195,050 + -0.8 3,195,051 - +0.2 3,195,103 + +0.7 3,195,106 + -0.3 3,195,171 + -2.0 3,195,217 + -0.1 3,195,217 + -0.0 3,195,217 + -0.2 3,195,318 + +0.2 3,195,319 - -0.4 3,195,378 - -0.8 3,195,559 - PS417_14630 0.30 +1.0 3,195,965 + +0.1 3,195,968 + -1.0 3,195,968 + +0.5 3,195,968 + +0.8 3,195,968 + -2.3 3,196,100 + PS417_14635 0.23 -0.6 3,196,100 + PS417_14635 0.23 -1.1 3,196,166 + PS417_14635 0.31 -1.1 3,196,386 - PS417_14635 0.55 -0.9 3,196,487 + PS417_14635 0.66 +0.1 3,196,488 - PS417_14635 0.66 -2.7 3,196,488 - PS417_14635 0.66 +1.0 3,196,718 + -0.1 3,196,719 - +0.2 3,196,813 + +0.5 3,196,880 + -0.8 3,196,915 + +0.3 3,197,191 + -0.2 3,197,191 + -1.7 3,197,192 - -0.6
Or see this region's nucleotide sequence