Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10675 and PS417_10680 are separated by 152 nucleotides PS417_10680 and PS417_10685 are separated by 27 nucleotides PS417_10685 and PS417_10690 are separated by 16 nucleotides
PS417_10675: PS417_10675 - TetR family transcriptional regulator, at 2,280,436 to 2,281,074
_10675
PS417_10680: PS417_10680 - enoyl-CoA hydratase, at 2,281,227 to 2,282,018
_10680
PS417_10685: PS417_10685 - acyl-CoA dehydrogenase, at 2,282,046 to 2,283,206
_10685
PS417_10690: PS417_10690 - acyl-CoA synthetase, at 2,283,223 to 2,285,331
_10690
Position (kb)
2282
2283
2284 Strain fitness (log2 ratio)
-1
0
1 at 2281.208 kb on - strand at 2281.232 kb on + strand at 2281.232 kb on + strand at 2281.233 kb on - strand at 2281.474 kb on - strand, within PS417_10680 at 2281.735 kb on + strand, within PS417_10680 at 2281.736 kb on - strand, within PS417_10680 at 2281.809 kb on - strand, within PS417_10680 at 2282.017 kb on - strand at 2282.039 kb on + strand at 2282.040 kb on - strand at 2282.209 kb on - strand, within PS417_10685 at 2282.209 kb on - strand, within PS417_10685 at 2282.253 kb on + strand, within PS417_10685 at 2282.565 kb on + strand, within PS417_10685 at 2282.565 kb on + strand, within PS417_10685 at 2282.565 kb on + strand, within PS417_10685 at 2282.565 kb on + strand, within PS417_10685 at 2282.682 kb on + strand, within PS417_10685 at 2282.886 kb on + strand, within PS417_10685 at 2282.886 kb on + strand, within PS417_10685 at 2283.108 kb on - strand at 2283.168 kb on - strand at 2283.222 kb on + strand at 2283.222 kb on + strand at 2283.222 kb on + strand at 2283.223 kb on - strand at 2283.223 kb on - strand at 2283.223 kb on - strand at 2283.223 kb on - strand at 2283.223 kb on - strand at 2283.223 kb on - strand at 2283.414 kb on - strand at 2283.482 kb on + strand, within PS417_10690 at 2283.509 kb on + strand, within PS417_10690 at 2283.514 kb on + strand, within PS417_10690 at 2283.515 kb on - strand, within PS417_10690 at 2283.628 kb on + strand, within PS417_10690 at 2283.760 kb on + strand at 2283.760 kb on + strand, within PS417_10690 at 2283.761 kb on - strand, within PS417_10690 at 2283.761 kb on - strand, within PS417_10690 at 2283.761 kb on - strand, within PS417_10690
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,281,208 - +0.2 2,281,232 + +0.9 2,281,232 + +0.3 2,281,233 - -1.1 2,281,474 - PS417_10680 0.31 -0.6 2,281,735 + PS417_10680 0.64 +0.0 2,281,736 - PS417_10680 0.64 +0.2 2,281,809 - PS417_10680 0.73 +0.3 2,282,017 - -1.4 2,282,039 + -1.4 2,282,040 - +0.2 2,282,209 - PS417_10685 0.14 -1.4 2,282,209 - PS417_10685 0.14 -0.6 2,282,253 + PS417_10685 0.18 -0.7 2,282,565 + PS417_10685 0.45 -1.2 2,282,565 + PS417_10685 0.45 -0.6 2,282,565 + PS417_10685 0.45 -0.1 2,282,565 + PS417_10685 0.45 -0.8 2,282,682 + PS417_10685 0.55 -0.3 2,282,886 + PS417_10685 0.72 -0.3 2,282,886 + PS417_10685 0.72 +0.0 2,283,108 - +0.8 2,283,168 - -0.2 2,283,222 + +0.2 2,283,222 + -0.2 2,283,222 + -0.1 2,283,223 - -0.9 2,283,223 - +0.4 2,283,223 - +0.3 2,283,223 - +0.2 2,283,223 - +0.5 2,283,223 - +0.7 2,283,414 - -0.3 2,283,482 + PS417_10690 0.12 -0.9 2,283,509 + PS417_10690 0.14 +0.7 2,283,514 + PS417_10690 0.14 -1.4 2,283,515 - PS417_10690 0.14 +0.7 2,283,628 + PS417_10690 0.19 -0.4 2,283,760 + +1.3 2,283,760 + PS417_10690 0.25 -0.7 2,283,761 - PS417_10690 0.26 -0.0 2,283,761 - PS417_10690 0.26 -0.3 2,283,761 - PS417_10690 0.26 +0.6
Or see this region's nucleotide sequence