Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10135 and PS417_10140 are separated by 3 nucleotides PS417_10140 and PS417_10145 are separated by 47 nucleotides PS417_10145 and PS417_10150 are separated by 27 nucleotides
PS417_10135: PS417_10135 - phage tail protein, at 2,170,113 to 2,170,769
_10135
PS417_10140: PS417_10140 - hypothetical protein, at 2,170,773 to 2,171,153
_10140
PS417_10145: PS417_10145 - hypothetical protein, at 2,171,201 to 2,171,473
_10145
PS417_10150: PS417_10150 - tail protein, at 2,171,501 to 2,174,587
_10150
Position (kb)
2171
2172 Strain fitness (log2 ratio)
-2
-1
0
1 at 2170.461 kb on + strand, within PS417_10135 at 2170.462 kb on - strand, within PS417_10135 at 2170.768 kb on - strand at 2171.340 kb on - strand, within PS417_10145 at 2171.340 kb on - strand, within PS417_10145 at 2171.340 kb on - strand, within PS417_10145 at 2171.486 kb on - strand at 2171.486 kb on - strand at 2171.931 kb on - strand, within PS417_10150 at 2171.931 kb on - strand at 2171.964 kb on - strand, within PS417_10150 at 2172.040 kb on - strand, within PS417_10150 at 2172.040 kb on - strand, within PS417_10150 at 2172.200 kb on + strand, within PS417_10150 at 2172.200 kb on + strand, within PS417_10150 at 2172.311 kb on - strand, within PS417_10150 at 2172.319 kb on + strand, within PS417_10150 at 2172.319 kb on + strand, within PS417_10150 at 2172.323 kb on - strand, within PS417_10150 at 2172.382 kb on + strand, within PS417_10150 at 2172.401 kb on - strand, within PS417_10150 at 2172.466 kb on + strand, within PS417_10150 at 2172.467 kb on - strand, within PS417_10150
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,170,461 + PS417_10135 0.53 -0.1 2,170,462 - PS417_10135 0.53 -0.2 2,170,768 - +0.5 2,171,340 - PS417_10145 0.51 -0.9 2,171,340 - PS417_10145 0.51 -1.3 2,171,340 - PS417_10145 0.51 -2.2 2,171,486 - +1.3 2,171,486 - +0.4 2,171,931 - PS417_10150 0.14 -0.1 2,171,931 - +0.7 2,171,964 - PS417_10150 0.15 +1.0 2,172,040 - PS417_10150 0.17 -1.3 2,172,040 - PS417_10150 0.17 -1.3 2,172,200 + PS417_10150 0.23 +1.7 2,172,200 + PS417_10150 0.23 -0.9 2,172,311 - PS417_10150 0.26 -0.7 2,172,319 + PS417_10150 0.26 +0.7 2,172,319 + PS417_10150 0.26 +0.1 2,172,323 - PS417_10150 0.27 +0.1 2,172,382 + PS417_10150 0.29 -1.7 2,172,401 - PS417_10150 0.29 +1.2 2,172,466 + PS417_10150 0.31 -0.2 2,172,467 - PS417_10150 0.31 -0.5
Or see this region's nucleotide sequence