Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06585 and PS417_06590 are separated by 23 nucleotides PS417_06590 and PS417_06595 are separated by 260 nucleotides PS417_06595 and PS417_06600 are separated by 1 nucleotides PS417_06600 and PS417_06605 overlap by 4 nucleotides
PS417_06585: PS417_06585 - hypothetical protein, at 1,458,384 to 1,459,262
_06585
PS417_06590: PS417_06590 - membrane protein, at 1,459,286 to 1,459,582
_06590
PS417_06595: PS417_06595 - ABC transporter ATP-binding protein, at 1,459,843 to 1,460,544
_06595
PS417_06600: PS417_06600 - leucine/isoleucine/valine transporter ATP-binding subunit, at 1,460,546 to 1,461,313
_06600
PS417_06605: PS417_06605 - leucine/isoleucine/valine transporter permease subunit, at 1,461,310 to 1,462,563
_06605
Position (kb)
1459
1460
1461 Strain fitness (log2 ratio)
-2
-1
0
1 at 1459.058 kb on - strand, within PS417_06585 at 1459.081 kb on + strand, within PS417_06585 at 1459.111 kb on + strand, within PS417_06585 at 1459.111 kb on + strand at 1459.111 kb on + strand, within PS417_06585 at 1459.111 kb on + strand, within PS417_06585 at 1459.111 kb on + strand, within PS417_06585 at 1459.267 kb on - strand at 1459.639 kb on - strand at 1460.367 kb on - strand, within PS417_06595 at 1460.513 kb on + strand at 1460.547 kb on + strand at 1460.563 kb on - strand at 1461.151 kb on - strand, within PS417_06600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,459,058 - PS417_06585 0.77 +0.0 1,459,081 + PS417_06585 0.79 -1.4 1,459,111 + PS417_06585 0.83 -0.3 1,459,111 + +1.6 1,459,111 + PS417_06585 0.83 -0.5 1,459,111 + PS417_06585 0.83 -2.2 1,459,111 + PS417_06585 0.83 -0.8 1,459,267 - -1.3 1,459,639 - +0.2 1,460,367 - PS417_06595 0.75 -0.0 1,460,513 + -0.7 1,460,547 + -0.6 1,460,563 - -1.0 1,461,151 - PS417_06600 0.79 -2.1
Or see this region's nucleotide sequence